中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
2期
106-109
,共4页
沈斌%屠巧峰%冷建杭%吴黎明%陈洵礼
瀋斌%屠巧峰%冷建杭%吳黎明%陳洵禮
침빈%도교봉%랭건항%오려명%진순례
皮炎,特应性%白细胞介素17%Th17细胞
皮炎,特應性%白細胞介素17%Th17細胞
피염,특응성%백세포개소17%Th17세포
Dermatitis,atopic%Interleukin-17%Th17 cells
目的 探讨特应性皮炎(atopic dermatitis,AD)患者外周血白介素17蛋白和mRNA水平及辅助性T细胞17(Th17)表达及其意义.方法 用酶联免疫吸附测定法(ELISA)检测63例AD患者及30例正常人对照血浆中IL-17蛋白水平.实时荧光定量RT-PCR检测两组外周血中IL-17 mRNA表达水平.流式细胞仪检测AD患者和对照者外周血单一核细胞中Th17细胞的比例.分析外周血Th17细胞比例和IL-17水平与AD患者病情严重度的相关性.结果 急性期AD患者外周血中Th17细胞比例为1.83％±0.47％,明显高于正常人对照组(0.85％±0.45％,t=4.128,P＜ 0.01)和慢性期组(1.12％±0.69％,t=2.439,P＜0.05).AD组外周血中血浆IL-17水平为98.37 ng/L,高于正常人组63.75 ng/L,U=168,P＜0.05.AD组外周血IL- 17mRNA表达水平高于正常人对照组.AD患者外周血Th17细胞比例和血IL-17蛋白水平与湿疹面积及严重度指数(EASI)积分均呈显著正相关(r=0.681,P＜ 0.01；r=0.427,P＜ 0.05).结论急性期AD患者外周血Th17细胞比例显著升高,IL-17蛋白分泌及基因表达水平存在异常,与病情严重度明显相关,提示IL-17和Th17细胞可能在AD发病中起作用.
目的 探討特應性皮炎(atopic dermatitis,AD)患者外週血白介素17蛋白和mRNA水平及輔助性T細胞17(Th17)錶達及其意義.方法 用酶聯免疫吸附測定法(ELISA)檢測63例AD患者及30例正常人對照血漿中IL-17蛋白水平.實時熒光定量RT-PCR檢測兩組外週血中IL-17 mRNA錶達水平.流式細胞儀檢測AD患者和對照者外週血單一覈細胞中Th17細胞的比例.分析外週血Th17細胞比例和IL-17水平與AD患者病情嚴重度的相關性.結果 急性期AD患者外週血中Th17細胞比例為1.83％±0.47％,明顯高于正常人對照組(0.85％±0.45％,t=4.128,P＜ 0.01)和慢性期組(1.12％±0.69％,t=2.439,P＜0.05).AD組外週血中血漿IL-17水平為98.37 ng/L,高于正常人組63.75 ng/L,U=168,P＜0.05.AD組外週血IL- 17mRNA錶達水平高于正常人對照組.AD患者外週血Th17細胞比例和血IL-17蛋白水平與濕疹麵積及嚴重度指數(EASI)積分均呈顯著正相關(r=0.681,P＜ 0.01；r=0.427,P＜ 0.05).結論急性期AD患者外週血Th17細胞比例顯著升高,IL-17蛋白分泌及基因錶達水平存在異常,與病情嚴重度明顯相關,提示IL-17和Th17細胞可能在AD髮病中起作用.
목적 탐토특응성피염(atopic dermatitis,AD)환자외주혈백개소17단백화mRNA수평급보조성T세포17(Th17)표체급기의의.방법 용매련면역흡부측정법(ELISA)검측63례AD환자급30례정상인대조혈장중IL-17단백수평.실시형광정량RT-PCR검측량조외주혈중IL-17 mRNA표체수평.류식세포의검측AD환자화대조자외주혈단일핵세포중Th17세포적비례.분석외주혈Th17세포비례화IL-17수평여AD환자병정엄중도적상관성.결과 급성기AD환자외주혈중Th17세포비례위1.83％±0.47％,명현고우정상인대조조(0.85％±0.45％,t=4.128,P＜ 0.01)화만성기조(1.12％±0.69％,t=2.439,P＜0.05).AD조외주혈중혈장IL-17수평위98.37 ng/L,고우정상인조63.75 ng/L,U=168,P＜0.05.AD조외주혈IL- 17mRNA표체수평고우정상인대조조.AD환자외주혈Th17세포비례화혈IL-17단백수평여습진면적급엄중도지수(EASI)적분균정현저정상관(r=0.681,P＜ 0.01；r=0.427,P＜ 0.05).결론급성기AD환자외주혈Th17세포비례현저승고,IL-17단백분비급기인표체수평존재이상,여병정엄중도명현상관,제시IL-17화Th17세포가능재AD발병중기작용.
Objective To determine the protein and mRNA expression levels of interleukin-17 (IL-17) and proportions of Th17 cells in peripheral blood of patients with atopic dermatitis (AD),and to analyze their clinical significance.Methods Venous blood samples were obtained from 63 patients with AD and 30 normal human controls.Enzyme linked immunosorbent assay was performed to measure the plasma level of IL-17 protein,real time fluorescence reverse transcription (RT)-PCR to detect the mRNA expression level of IL-17 in peripheral blood,flow cytometry to determine the percentage of Th17 cells in peripheral blood mononuclear cells (PBMCs).Spearman's rank correlation analysis was performed to assess the relationship of peripheral blood Th17 cell percentage and IL-17 levels with disease severity in patients with AD.Results The percentage of Th17 cells in PBMCs was 1.83％ ± 0.47％ in patients with acute AD,significantly higher than that in normal human controls (0.85％ ± 0.45％,t =4.128,P ＜ 0.01 ) and in patients with chronic AD ( 1.12％ ± 0.69％,t =2.439,P ＜ 0.05).There was a significant increase in the plasma level (98.37 vs.63.75 ng/L,U =168,P ＜ 0.05) and mRNA expression level of IL-17 in peripheral blood in patients with AD compared with normal controls.Both the Th17 cell percentage and plasma IL-17 level were positively correlated with eczema area and severity index (EASI) score in patients with AD (r =0.681,P ＜ 0.01;r =0.427,P ＜ 0.05).Conclusions There is an elevated proportion of Th17 cells and an abnormal expression of IL-17 protein and mRNA in peripheral blood from patients with acute AD,which are positively correlated with disease severity,implicating the possible roles of IL-17 and Th17 cells in the pathogenesis of AD.
