CAJ | 학술논문

目的探讨线粒体分裂对胰岛β细胞功能的影响.方法通过可诱导表达野生型动力相关蛋白1(Drp-1 WT)基因和突变型Drp-1(Drp-1K38A)基因的大鼠INS-1 β细胞,分析不同葡萄糖条件下,线粒体分裂对胰岛β细胞功能的影响.结果在高糖条件下,强力霉素诱导后的Drp-1WT细胞线粒体分裂过程增强,网络状结构部分断裂,多呈点状结构,细胞胰岛素分泌功能降低(P<0.01),线粒体膜电位降低(P<0.05),细胞内ATP含量减少(P<0.05),细胞色素C表达增加,而在Drp-1K38A细胞中,上述变化明显减轻.结论线粒体分裂增强可抑制胰岛β细胞功能.
목적 탐토선립체분렬대이도β세포공능적영향.방법 통과가유도표체야생형동력상관단백1(Drp-1 WT)기인화돌변형Drp-1(Drp-1K38A)기인적대서INS-1 β세포,분석불동포도당조건하,선립체분렬대이도β세포공능적영향.결과 재고당조건하,강력매소유도후적Drp-1WT세포선립체분렬과정증강,망락상결구부분단렬,다정점상결구,세포이도소분비공능강저(P<0.01),선립체막전위강저(P<0.05),세포내ATP함량감소(P<0.05),세포색소C표체증가,이재Drp-1K38A세포중,상술변화명현감경.결론 선립체분렬증강가억제이도β세포공능.
Objective To investigate the effect of mitochondrial fission on the function of pancreatic β cells.Methods INS-1 stable cell lines allowing inducible expression of either wild-type dynamin-related protein 1 (Drp-1 WT)or its dominant-negative mutant(Drp-1 K38A)were used.The effect of mitochondrial fission on the function of pancreatic β cells were investigated under different concentrations of glucose.Results There were increased mitochondrial fission and disintegration of the mitochondrial reticulum into multiple punctiform organelles in Drp-1 WT cells induced with doxycycline under high glucose condition.Insulin secretion(P<0.01),mitochondrial membrane potential(P<0.05),and ATP content(P<0.05)were decreased and cytochrome C expression was increased after the expression of Drp-1 WT under high glucose condition while these changes were markedly mild in Drp-1 K38A expression cells.Conclusion The increased mitochondrial fission inhibits pancreatic β cell function.