CAJ | 학술논문

目的探讨慢性不可预见应激大鼠的听觉惊跳反射和弱刺激抑制变化情况以及帕罗西汀对其的影响.方法将24只大鼠按随机数字表法分为阴性对照组(8只)、慢性不可预见应激组(8只)和帕罗西汀组(8只).阴性对照组:静养21 d后给予蒸馏水灌胃；慢性不可预见应激组:先给予慢性不可预见应激21d后再给予蒸馏水灌胃21 d；帕罗西汀组:先给予慢性不可预见应激21 d后再给予帕罗西汀灌胃21 d.3组大鼠均接受体质量测量、自发活动、糖水偏好、惊跳反射和弱刺激抑制测试.结果 (1)慢性不可预见应激组大鼠体质量[(380.50±22.23)g]、10 min旷场自发活动[ (5765.57 ±2942.28) mm]、糖水摄入量[(19.09 ±7.16) ml/kg]均低于阴性对照组[(426.38±33.73)g、(12 272.15±2343.02) mm(42.58±11.68) ml/kg,P＜0.01];帕罗西汀组体质量[ (353.62 ±29.37)g]低于阴性对照组(P＜0.01).(2)惊跳反射实验结果3组大鼠之间差异无统计学意义(P＞0.05).(3)慢性不可预见应激组大鼠弱刺激抑制[( 30.50±14.84)％]小于阴性对照组和帕罗西汀组[ (57.80±13.32)％、(42.32±15.82)％],帕罗西汀组弱刺激抑制小于阴性对照组,差异均有统计学意义(P ＜0.01)；82 dB时的弱刺激抑制高于70、64、58 dB时的弱刺激抑制(P＜0.01)；76 dB时的弱刺激抑制高于64、58 dB时的弱刺激抑制(P ＜0.01)；70 dB时的弱刺激抑制高于58 dB时的弱刺激抑制(P＜0.05).结论随着弱刺激强度增加,弱刺激抑制逐渐增加；慢性不可预见应激大鼠存在弱刺激抑制的缺失,帕罗西汀能够缓解这一状况.
목적 탐토만성불가예견응격대서적은각량도반사화약자격억제변화정황이급파라서정대기적영향.방법 장24지대서안수궤수자표법분위음성대조조(8지)、만성불가예견응격조(8지)화파라서정조(8지).음성대조조:정양21 d후급여증류수관위；만성불가예견응격조:선급여만성불가예견응격21d후재급여증류수관위21 d；파라서정조:선급여만성불가예견응격21 d후재급여파라서정관위21 d.3조대서균접수체질량측량、자발활동、당수편호、량도반사화약자격억제측시.결과 (1)만성불가예견응격조대서체질량[(380.50±22.23)g]、10 min광장자발활동[ (5765.57 ±2942.28) mm]、당수섭입량[(19.09 ±7.16) ml/kg]균저우음성대조조[(426.38±33.73)g、(12 272.15±2343.02) mm(42.58±11.68) ml/kg,P＜0.01];파라서정조체질량[ (353.62 ±29.37)g]저우음성대조조(P＜0.01).(2)량도반사실험결과3조대서지간차이무통계학의의(P＞0.05).(3)만성불가예견응격조대서약자격억제[( 30.50±14.84)％]소우음성대조조화파라서정조[ (57.80±13.32)％、(42.32±15.82)％],파라서정조약자격억제소우음성대조조,차이균유통계학의의(P ＜0.01)；82 dB시적약자격억제고우70、64、58 dB시적약자격억제(P＜0.01)；76 dB시적약자격억제고우64、58 dB시적약자격억제(P ＜0.01)；70 dB시적약자격억제고우58 dB시적약자격억제(P＜0.05).결론 수착약자격강도증가,약자격억제축점증가；만성불가예견응격대서존재약자격억제적결실,파라서정능구완해저일상황.
Objective To explore the deficits of prepulse inhibition (PPI) of acoustic startle reflex (ASR) in chronic unpredicted stress (CUS) rats,and the effects of paroxetine on ASR and PPI.Methods Twenty-four Sprague Dawley rats were randomly divided into control group,CUS group and CUS + paroxetine group.The rats in control group were left undisturbed in their home cage for 21 days and then administration of distilled water (5 ml/kg) for consecutive 21 days; the rats in CUS group exposed to 21 consecutive days of CUS and then treated with distilled water (5 ml/kg) for consecutive 21 days; the rats in CUS + paroxetine group exposed to 21 consecutive days of CUS and then treated with paroxetine ( 10 mg/kg) mixed with distilled water (5 ml/kg) for consecutive 21 days.Behavioral changes in these rats were analyzed in weight change,open field test,sucrose preference,ASR and PPI paradigm.Results ( 1 ) Compared with control group,weight [ (380.50 ± 22.23 ) g ],10-min total traveling distance [ ( 5765.57 ± 2942.28 ) mm ],sucrose preference [ ( 19.09 ±7.16) ml/kg] were lower (P ＜0.01 ) in the CUS group.Weight of CUS + paroxetine group [(353.62±29.37) g was lower than control group (P＜0.01). (2) There were no differences of ASR among control group, CUS group, and CUS + paroxetine group ( P ＞ 0.05 ).(3) Compared with control group and CUS + paroxetine group,PPI in CUS group [ (30.50 ± 14.84)％ ]was reduced (P ＜0.01 ),PPI in CUS + paroxetine group was lower than control group (P ＜0.01 ).PPI of 82 dB [ (56.00 ± 17.10) ％ ] was higher than that of 70,64,58 dB (P ＜ 0.01 ) ; PPI of 76 dB [ (49.76 ±18.03)％] was higher than that of 64,58dB (P＜0.01); PPI of 70 dB [(43.17±15.22)％] was higher than that of 58 dB ( P ＜ 0.05 ).Conclusion The results suggest there be impaired PPI in chronic unpredieted stress rats,while paroxetine could alleviate the deficits.