CAJ | 학술논문

目的检测叉头翼状螺旋转录因子(forkhead/winged helix transcription factor p3,Foxp3)阳性调节性T细胞(regulatory T cells,Treg)在口腔扁平苔藓(oral lichen planus,OLP)中的表达分布特征及变化,探讨其对OLP发生、发展的影响,以期为研究OLP发病机制及治疗提供参考.方法应用免疫组织化学双标记技术检测35例0LP患者和19例正常对照组口腔黏膜组织中CD4+ Foxp3+、CD25+Foxp3+ Treg的表达,并分析CD4+ Foxp3+/CD4T细胞的比值变化.结果 OLP组病损组织中有大量CD4+Foxp3+、CD25+Foxp3+ Treg浸润,每高倍视野所含阳性细胞数分别为(39.58±11.08)、(35.50±17.74),较对照组[每高倍视野细胞数分别为(1.88±0.51)、(2.50±0.65)]显著增高(P＜0.01)；OLP组织中CD4+ Foxp3+/CD4+T细胞比值为(21.11±8.87)％,较正常对照组[(38.89±8.84)％]显著降低(P＜0.01)；糜烂型OLP组和网纹型OLP组CD4+ Foxp3+、CD25+ Foxp3+ Treg数量差异无统计学意义(P＞0.05),但两组间CD4 Foxp3+/CD4T细胞的比值差异有统计学意义(P＜0.05).结论 OLP组织中Foxp3 +Treg的数量及其与效应性T细胞的比值发生了改变,两者之间的平衡失调与OLP的发生及临床分型相关.
목적 검측차두익상라선전록인자(forkhead/winged helix transcription factor p3,Foxp3)양성조절성T세포(regulatory T cells,Treg)재구강편평태선(oral lichen planus,OLP)중적표체분포특정급변화,탐토기대OLP발생、발전적영향,이기위연구OLP발병궤제급치료제공삼고.방법 응용면역조직화학쌍표기기술검측35례0LP환자화19례정상대조조구강점막조직중CD4+ Foxp3+、CD25+Foxp3+ Treg적표체,병분석CD4+ Foxp3+/CD4T세포적비치변화.결과 OLP조병손조직중유대량CD4+Foxp3+、CD25+Foxp3+ Treg침윤,매고배시야소함양성세포수분별위(39.58±11.08)、(35.50±17.74),교대조조[매고배시야세포수분별위(1.88±0.51)、(2.50±0.65)]현저증고(P＜0.01)；OLP조직중CD4+ Foxp3+/CD4+T세포비치위(21.11±8.87)％,교정상대조조[(38.89±8.84)％]현저강저(P＜0.01)；미란형OLP조화망문형OLP조CD4+ Foxp3+、CD25+ Foxp3+ Treg수량차이무통계학의의(P＞0.05),단량조간CD4 Foxp3+/CD4T세포적비치차이유통계학의의(P＜0.05).결론 OLP조직중Foxp3 +Treg적수량급기여효응성T세포적비치발생료개변,량자지간적평형실조여OLP적발생급림상분형상관.
Objective To evaluate the expression and distribution of forkhead/winged helix transcription factor p3 (Foxp3) + regulatory T cells (Treg) in oral lichen planus (OLP) and to explore the role of Foxp3 + Treg cells in the pathogenesis of OLP.Methods Immunohistochemistry double stainings ( CD4+ Foxp3 + and CD25+ Foxp3 + ) were performed on tissue sections of 35 cases of OLP,which was compared with 19 normal oral mucosa controls.A further analysis was carried out on the percentages of CD4+ Foxp3 + cells in CD4+ T cells.Results OLP biopsy samples showed a strong infiltration of CD4+ Foxp3 + Treg cell and CD25+ Foxp3 + Treg.A significantly higher frequency of CD4+ Foxp3 + Treg ( 39.58 ± 11.08 ),CD25+ Foxp3 + Treg (35.50 ± 17.74 ) per high magnification field,could be observed in OLP,compared with control group ( 1.88 ±0.51,2.5 ±0.65 respectively) (P ＜0.01 ).Whereas in OLP group,the proportion of Foxp3 + cells in CD4+T cells[ (21.11 ±8.87)％ ] was significantly lower than that in control group[ (38.89 ±8.84 )％ ] ( P ＜ 0.01 ).Between the erosive OLP group and the reticular OLP group,the quantity of CD4+ Foxp3 + Treg or CD25+ Foxp3 + Treg showed no significant difference.But in the erosive OLP group,the proportion of Foxp3 + cells in CD4+ T cells was significantly lower than that in reticular OLP group (P ＜ 0.05 ).Conclusions The imbalance between regulatory T cells and effector T cells occurs within OLP lesions,which may play a role in pathogenesis of OLP.