中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
29期
2030-2035
,共6页
茹国庆%ZHAO Zhong-sheng%汤琪乐%XU Wen-juan
茹國慶%ZHAO Zhong-sheng%湯琪樂%XU Wen-juan
여국경%ZHAO Zhong-sheng%탕기악%XU Wen-juan
胃肿瘤%成纤维细胞生长因子'碱性%肝细胞生长因子%预后
胃腫瘤%成纖維細胞生長因子'堿性%肝細胞生長因子%預後
위종류%성섬유세포생장인자'감성%간세포생장인자%예후
Stomach neoplasm%Fibroblast growth factor,basic%Hepatocyte growth factor,basic%Prognosis
目的 研究碱性成纤维细胞生长因子(Bfgf)、肝细胞生长因子(HGF)Mrna在胃癌组织中的表达及其与肿瘤内微血管密度(IMD)、增殖细胞核抗原(PCNA)、浸润转移和生存期的关系.方法 应用原位杂交和免疫组织化学技术,检测118例胃癌组织中Bfgf、HGF及CD34、PCNA的表达.结果 Bfgf Mrna和HGF Mrna在胃癌中的阳性表达率分别为57.6%和52.5%;胃癌的侵袭深度、脉管累犯、淋巴结及远处转移均与Bfgf和HGF Mrna的表达水平有关(均P<0.05).Bfgf和HGF Mrna阳性表达病例的平均血管数分别为(46.7±13.3)个/0.72 mm2和(46.6±14.0)个/0.72 mm2,均分别明显高于阴性表达病例的平均血管数(34.6±15.2)个/0.72 mm2(t=4.62,P=0.000)和(36.0±14.9)个/0.72 mm2(t=4.01,P=0.000);PCNA标记指数分别为66.5±13.6和67.6±13.4,均分别明显高于阴性表达病例的PCNA标记指数55.0±11.0(t=4.91,P=0.000)和54.8±10.6(t=5.83,P=0.000).Bfgf Mrna和HGF Mrna阳性表达病例的平均生存时间及5年生存率均明显低于阴性表达的病例.结论 Bfgf和HGF可促进胃癌血管生成,促进肿瘤细胞的增殖,并参与肿瘤浸润转移过程,可以作为反映胃癌生物学行为、判断预后及指导治疗都具有重要的意义.
目的 研究堿性成纖維細胞生長因子(Bfgf)、肝細胞生長因子(HGF)Mrna在胃癌組織中的錶達及其與腫瘤內微血管密度(IMD)、增殖細胞覈抗原(PCNA)、浸潤轉移和生存期的關繫.方法 應用原位雜交和免疫組織化學技術,檢測118例胃癌組織中Bfgf、HGF及CD34、PCNA的錶達.結果 Bfgf Mrna和HGF Mrna在胃癌中的暘性錶達率分彆為57.6%和52.5%;胃癌的侵襲深度、脈管纍犯、淋巴結及遠處轉移均與Bfgf和HGF Mrna的錶達水平有關(均P<0.05).Bfgf和HGF Mrna暘性錶達病例的平均血管數分彆為(46.7±13.3)箇/0.72 mm2和(46.6±14.0)箇/0.72 mm2,均分彆明顯高于陰性錶達病例的平均血管數(34.6±15.2)箇/0.72 mm2(t=4.62,P=0.000)和(36.0±14.9)箇/0.72 mm2(t=4.01,P=0.000);PCNA標記指數分彆為66.5±13.6和67.6±13.4,均分彆明顯高于陰性錶達病例的PCNA標記指數55.0±11.0(t=4.91,P=0.000)和54.8±10.6(t=5.83,P=0.000).Bfgf Mrna和HGF Mrna暘性錶達病例的平均生存時間及5年生存率均明顯低于陰性錶達的病例.結論 Bfgf和HGF可促進胃癌血管生成,促進腫瘤細胞的增殖,併參與腫瘤浸潤轉移過程,可以作為反映胃癌生物學行為、判斷預後及指導治療都具有重要的意義.
목적 연구감성성섬유세포생장인자(Bfgf)、간세포생장인자(HGF)Mrna재위암조직중적표체급기여종류내미혈관밀도(IMD)、증식세포핵항원(PCNA)、침윤전이화생존기적관계.방법 응용원위잡교화면역조직화학기술,검측118례위암조직중Bfgf、HGF급CD34、PCNA적표체.결과 Bfgf Mrna화HGF Mrna재위암중적양성표체솔분별위57.6%화52.5%;위암적침습심도、맥관루범、림파결급원처전이균여Bfgf화HGF Mrna적표체수평유관(균P<0.05).Bfgf화HGF Mrna양성표체병례적평균혈관수분별위(46.7±13.3)개/0.72 mm2화(46.6±14.0)개/0.72 mm2,균분별명현고우음성표체병례적평균혈관수(34.6±15.2)개/0.72 mm2(t=4.62,P=0.000)화(36.0±14.9)개/0.72 mm2(t=4.01,P=0.000);PCNA표기지수분별위66.5±13.6화67.6±13.4,균분별명현고우음성표체병례적PCNA표기지수55.0±11.0(t=4.91,P=0.000)화54.8±10.6(t=5.83,P=0.000).Bfgf Mrna화HGF Mrna양성표체병례적평균생존시간급5년생존솔균명현저우음성표체적병례.결론 Bfgf화HGF가촉진위암혈관생성,촉진종류세포적증식,병삼여종류침윤전이과정,가이작위반영위암생물학행위、판단예후급지도치료도구유중요적의의.
Objective To investigate the mRNA expression of basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF) in gastric carcinoma and correlation thereof with intratumoral microvascular density (IMD), proliferating cell nuclear antigen labeling index (PCNA LI), tumor invasion and meatastasis, and patients' survival. Methods In situ hybridization and immunohistochemistry were used to test the expression of bFGF and HGF mRNA and protein expression of CD34 and PCNA in 118 specimens of gastric carcinoma. Results The positive rates of bFGF mRNA and HGF mRNA were 57.6% and 52.5% respectively; the positive expression rates of bFGF mRNA and HGF mRNA of the cases at the stage T3 -14 and those with vessel invasion, lymph node metastasis, and distant metastasis were all significantly higher than the positive expression rates of the cases at the stage T1 - T2, and those without lymph node metastasis, vessel invasion, and distant metastasis ( all P < O. 05). The mean IMD levels of the patients positive in bFGF and HGF mRNA expression were (46.63 ± 13.96) and (46.73 ± 13.34) pieces/ 0.72 mm2, both significantly higher than those of the patients negative in bFGF and HGF mRNA expression [(34.56±15.16) and (35.98±14.92) pieces/0.72 mm2) respectively, t =4.62,P=0.000, and t = 4. 01 ,P=0.000]. The PCNA LI of the of the patients positive in bFGF and HGF mRNA expression was (66.53 ± 13.61 ) and (67.56 ± 13.41 ) respectively, both significantly higher5 than those of patients negative in bFGF and HGF mRNA expression [ (54.79 ± 10.57) and (55.04 ± 11. 01 ) respectively, t = 5. 83, P =0.000, and t =4.91, P =0.000] . The 5-year survival rates of the patients negative in bFGF and HGF mRNA expression were 60.00% and 67.90% respectively, both significantly higher than those of the patients positive in bFGF and HGF mRNA expression (30. 90% and 21.00% respectively, both P = 0. 000). IMD, PCNA LI, and bFGF mRNA were all positively related to HGF mRNA ( all P = 0. 000).Conclusions bFGF and HGF promote angiogenesis and proliferation of gastric cancer, and in involved in tumor invasion and metastasis. They can be used as markers of prognosis of gastric cancer in clinical practice.
