CAJ | 학술논문

目的研究尿道致病性大肠埃希菌(UPEC)菌株132与人膀胱上皮EJ细胞的相互作用,分析该菌株感染对EJ细胞基因表达谱的改变.方法 UPEC132感染EJ细胞,用倒置显微镜观察细菌与细胞的黏附,计算黏附率,并通过激光共聚焦显微镜观察UPEC132对细胞的侵袭.感染UPEC132的EJ细胞与未经细菌感染的细胞提取总RNA,用人类全基因组寡核苷酸微阵列芯片分析差异表达基因,并采用RT-PCR对基因芯片数据进行验证.结果 UPEC132能够黏附于EJ细胞表面,黏附率为(73.20±5.26)%;激光共聚焦显微镜观察发现部分细菌位于细胞内部,证实该菌对EJ细胞具有侵袭性.UPEC132感染后的EJ细胞与未经感染的细胞相比,共有28个基因上调,1个基因下调,主要涉及细胞增殖、炎症反应、细胞凋亡等相关基因.结论 UPEC与尿路上皮细胞的相互作用激活宿主细胞内部多种应答反应与信号转导途径,本研究为深入探索UPEC致病机制奠定基础.
목적 연구뇨도치병성대장애희균(UPEC)균주132여인방광상피EJ세포적상호작용,분석해균주감염대EJ세포기인표체보적개변.방법 UPEC132감염EJ세포,용도치현미경관찰세균여세포적점부,계산점부솔,병통과격광공취초현미경관찰UPEC132대세포적침습.감염UPEC132적EJ세포여미경세균감염적세포제취총RNA,용인류전기인조과핵감산미진렬심편분석차이표체기인,병채용RT-PCR대기인심편수거진행험증.결과 UPEC132능구점부우EJ세포표면,점부솔위(73.20±5.26)%;격광공취초현미경관찰발현부분세균위우세포내부,증실해균대EJ세포구유침습성.UPEC132감염후적EJ세포여미경감염적세포상비,공유28개기인상조,1개기인하조,주요섭급세포증식、염증반응、세포조망등상관기인.결론 UPEC여뇨로상피세포적상호작용격활숙주세포내부다충응답반응여신호전도도경,본연구위심입탐색UPEC치병궤제전정기출.
Objective To investigate the interaction between uropathogenic Escherichia coli (UPEC) and host uroepithelial cells, define the role uroepithelial cells play in initiating and modulating the host response to infection with UPEC strain. Methods The human bladder transitional epithelial EJ cells were evaluated for their capacities to allow the adherence and invasion by UPEC132, a clinical strain isolated from Tianjin, China, and a cDNA microarray for 22 000 human genes was used to identify the gene expression differences between EJ cells infected with UPEC132 and uninfected EJ cells. Results Microscope observation showed that UPEC132 could adhere to EJ cells with the adherence rate of (73.20 ± 5.26)%. And visualization by confocal microscope revealed that this microorganism could be seen within the cells. EJ cells infected with UPEC132 changed mRNA expression of a total of 29 genes, including 28 genes up-regulated and 1 gene down-regulated. Of these, regulators of growth and proliferation, cytokines, and modulators of apoptotic responses were the most prominent. Conclusion The gene expression profiling of EJ cells is affected by the infection of UPEC strain. The differentially expressed genes may contribute to further investigate the interaction of UPEC and uroepithelial cells.