中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
11期
727-731
,共5页
甄勇%许侃%陈学思%赵长稳%王柏%张德智%王宏磊%徐宁%于金录%罗祺
甄勇%許侃%陳學思%趙長穩%王柏%張德智%王宏磊%徐寧%于金錄%囉祺
견용%허간%진학사%조장은%왕백%장덕지%왕굉뢰%서저%우금록%라기
壳聚糖%成纤维细胞%颅内动脉瘤%组织工程
殼聚糖%成纖維細胞%顱內動脈瘤%組織工程
각취당%성섬유세포%로내동맥류%조직공정
Chitosan%Fibroblasts%Intracranial aneurysms%Tissue engineering
目的 探讨壳聚糖-甘油磷酸钠-成纤维细胞水凝胶作为颅内动脉瘤栓塞材料的可行性.方法 配制2%(w/v)壳聚糖与56%(w/v)甘油磷酸钠溶液,不同比例混合,测定混合液pH、37℃成胶时间及力学强度,体外观察兔皮肤成纤维细胞在不同配比壳聚糖-甘油磷酸钠水凝胶中生长状态,选择最佳比例.将Brdu标记的成纤维细胞与壳聚糖-甘油磷酸钠水凝胶混合栓塞兔颈动脉,评价组织相容性.最终栓塞兔动脉瘤模型.结果 当壳聚糖/甘油磷酸钠在7:1时,pH值为7.28,成胶时间为(260±18)s,力学强度可达14 kPa,细胞生存率最高,达(89.0±2.7)%.颈动脉栓塞,透视下可见混合物显影良好,局部组织切片显示(HE染色):1周时凝胶中散在较多细胞及钽粉,凝胶与血管壁边界较清,内皮细胞完整.4周时凝胶与血管壁边界不清,可见周边凝胶被细胞成分包绕降解.免疫荧光显示,4周标本中可见较多标记的成纤维细胞.兔动脉瘤栓塞术中见动脉瘤不显影,术后3d病理见动脉瘤被凝胶栓塞,内膜细胞形态正常,动脉壁内未见明显炎性细胞浸润.结论 短期观察,壳聚糖-甘油磷酸钠-成纤维细胞水凝胶可用于动脉瘤栓塞.
目的 探討殼聚糖-甘油燐痠鈉-成纖維細胞水凝膠作為顱內動脈瘤栓塞材料的可行性.方法 配製2%(w/v)殼聚糖與56%(w/v)甘油燐痠鈉溶液,不同比例混閤,測定混閤液pH、37℃成膠時間及力學彊度,體外觀察兔皮膚成纖維細胞在不同配比殼聚糖-甘油燐痠鈉水凝膠中生長狀態,選擇最佳比例.將Brdu標記的成纖維細胞與殼聚糖-甘油燐痠鈉水凝膠混閤栓塞兔頸動脈,評價組織相容性.最終栓塞兔動脈瘤模型.結果 噹殼聚糖/甘油燐痠鈉在7:1時,pH值為7.28,成膠時間為(260±18)s,力學彊度可達14 kPa,細胞生存率最高,達(89.0±2.7)%.頸動脈栓塞,透視下可見混閤物顯影良好,跼部組織切片顯示(HE染色):1週時凝膠中散在較多細胞及鐽粉,凝膠與血管壁邊界較清,內皮細胞完整.4週時凝膠與血管壁邊界不清,可見週邊凝膠被細胞成分包繞降解.免疫熒光顯示,4週標本中可見較多標記的成纖維細胞.兔動脈瘤栓塞術中見動脈瘤不顯影,術後3d病理見動脈瘤被凝膠栓塞,內膜細胞形態正常,動脈壁內未見明顯炎性細胞浸潤.結論 短期觀察,殼聚糖-甘油燐痠鈉-成纖維細胞水凝膠可用于動脈瘤栓塞.
목적 탐토각취당-감유린산납-성섬유세포수응효작위로내동맥류전새재료적가행성.방법 배제2%(w/v)각취당여56%(w/v)감유린산납용액,불동비례혼합,측정혼합액pH、37℃성효시간급역학강도,체외관찰토피부성섬유세포재불동배비각취당-감유린산납수응효중생장상태,선택최가비례.장Brdu표기적성섬유세포여각취당-감유린산납수응효혼합전새토경동맥,평개조직상용성.최종전새토동맥류모형.결과 당각취당/감유린산납재7:1시,pH치위7.28,성효시간위(260±18)s,역학강도가체14 kPa,세포생존솔최고,체(89.0±2.7)%.경동맥전새,투시하가견혼합물현영량호,국부조직절편현시(HE염색):1주시응효중산재교다세포급단분,응효여혈관벽변계교청,내피세포완정.4주시응효여혈관벽변계불청,가견주변응효피세포성분포요강해.면역형광현시,4주표본중가견교다표기적성섬유세포.토동맥류전새술중견동맥류불현영,술후3d병리견동맥류피응효전새,내막세포형태정상,동맥벽내미견명현염성세포침윤.결론 단기관찰,각취당-감유린산납-성섬유세포수응효가용우동맥류전새.
Objective To evaluate the feasibility of the injectable thermosetting chitosan -glycerophosphate-fibroblast (C-GP-FB) hydrogel as an embolizing material of intracranial aneurysm.Methods 2% chitosan solution and 56% glycerophosphate solution were mixed in different volume ratio to detect the pH, colloidization time at 37℃ ,and mechanic strength. Fibroblasts were isolated from the skin of a rabbit and cultured with C-GP hydrogel of different, ratios so as to determine the best ratio. Six rabbits underwent construction of aneurysm in the right branchioccphalic trunk. Three weeks later C-GP-FB hydrogel was infused into the aneurysm via microtube and balloon. X-ray photography was conducted soon to observe the embolizing effect. Three days, 1 week, and 4 weeks later tea rabbits were killed respectively with their aneurysms taken out to undergo HE staining and microscopy. Result When the ratio of C/GP was 7:1 at pH 7.28, the colloidization time was (260±18) sec, and the mechanic strength reached 14 kPa. The C/GP volume ratio of 7:1 was regarded as the best ratio. When the fibroblasts were cultured with the C-GP hydrogel with the C/GP volume ratio of 7:1 the survival rate of the fibroblasts reached the peak of (89±2.74)%. X-ray photography showed that image of aneurysm failed to be spotted immediately after the infusion of the C-GP hydrogel. HE staining and microscopy showed that C-GP-FB gel had fine visualization under X-ray. Histological section(HE stain) showed that 3 days after the infusion the aneurysms was embolized by the C-GP-FB hydrogel and no obvious inflammatory cell was seen in the arterial wall; the first week there were many cells in the gel, the boundary between the C-GP-FB gel and'vessel wall was clear, and the endotheliocytes were complete; and in the fourth week, the boundary between the gel and vessel wall was obscure, shght degradation could be observed in the edge of hydrogel, and immumofluorescence showed that there were many labeled cell in the gel . Conclusion In the short term view, C-GP-FB hydrogel can be used to embolize aneurysms, at least with an obvious short-term effect.