中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
41期
2943-2945
,共3页
缺血%再灌注损伤%脑%七氟烷
缺血%再灌註損傷%腦%七氟烷
결혈%재관주손상%뇌%칠불완
Ischemia%Reperfusion injury%Brain%Sevoflurane
目的 探讨七氟烷预处理是否减轻脑缺血再灌注引起的神经细胞凋亡.方法 36只雄性SD大鼠(250-300 g),随机分为3组:对照组(n=12);缺血再灌注组(n=12),动物建立大脑中动脉阻断再灌注模型;七氟烷预处理组(n=12),动物接受七氟烷预处理后建立大脑中动脉阻断再灌注模型.采用HE染色和透射电镜观察大鼠缺血再灌注脑区神经元的凋亡情况、原位细胞凋亡检测法计数神经元凋亡密度、免疫印迹(Western blot)检测缺血脑区半胱氨酸蛋白酶Caspase-3表达及活化.结果 HE染色、电镜的结果均提示七氟烷预处理组神经元凋亡比缺血再灌注组少、凋亡程度轻;神经元凋亡密度对照组为(13.0±1.4)个/0.1 mm~2、缺血再灌注组为(189.8±6.8)个/0.1 mm~2、七氟烷预处理组为(110.5±4.3)个/0.1 mm~2,两两比较,差异有统计学意义;缺血脑区Caspase-3前体及其20 000切割片段含量的相对灰度值分别为16.7±3.0、76.1±3.4、51.2±3.1及8.2±2.3、59.0±6.3、31.2±5.4.结论 七氟烷预处理可通过减轻脑缺血再灌注引起的神经细胞凋亡而产生保护作用.
目的 探討七氟烷預處理是否減輕腦缺血再灌註引起的神經細胞凋亡.方法 36隻雄性SD大鼠(250-300 g),隨機分為3組:對照組(n=12);缺血再灌註組(n=12),動物建立大腦中動脈阻斷再灌註模型;七氟烷預處理組(n=12),動物接受七氟烷預處理後建立大腦中動脈阻斷再灌註模型.採用HE染色和透射電鏡觀察大鼠缺血再灌註腦區神經元的凋亡情況、原位細胞凋亡檢測法計數神經元凋亡密度、免疫印跡(Western blot)檢測缺血腦區半胱氨痠蛋白酶Caspase-3錶達及活化.結果 HE染色、電鏡的結果均提示七氟烷預處理組神經元凋亡比缺血再灌註組少、凋亡程度輕;神經元凋亡密度對照組為(13.0±1.4)箇/0.1 mm~2、缺血再灌註組為(189.8±6.8)箇/0.1 mm~2、七氟烷預處理組為(110.5±4.3)箇/0.1 mm~2,兩兩比較,差異有統計學意義;缺血腦區Caspase-3前體及其20 000切割片段含量的相對灰度值分彆為16.7±3.0、76.1±3.4、51.2±3.1及8.2±2.3、59.0±6.3、31.2±5.4.結論 七氟烷預處理可通過減輕腦缺血再灌註引起的神經細胞凋亡而產生保護作用.
목적 탐토칠불완예처리시부감경뇌결혈재관주인기적신경세포조망.방법 36지웅성SD대서(250-300 g),수궤분위3조:대조조(n=12);결혈재관주조(n=12),동물건립대뇌중동맥조단재관주모형;칠불완예처리조(n=12),동물접수칠불완예처리후건립대뇌중동맥조단재관주모형.채용HE염색화투사전경관찰대서결혈재관주뇌구신경원적조망정황、원위세포조망검측법계수신경원조망밀도、면역인적(Western blot)검측결혈뇌구반광안산단백매Caspase-3표체급활화.결과 HE염색、전경적결과균제시칠불완예처리조신경원조망비결혈재관주조소、조망정도경;신경원조망밀도대조조위(13.0±1.4)개/0.1 mm~2、결혈재관주조위(189.8±6.8)개/0.1 mm~2、칠불완예처리조위(110.5±4.3)개/0.1 mm~2,량량비교,차이유통계학의의;결혈뇌구Caspase-3전체급기20 000절할편단함량적상대회도치분별위16.7±3.0、76.1±3.4、51.2±3.1급8.2±2.3、59.0±6.3、31.2±5.4.결론 칠불완예처리가통과감경뇌결혈재관주인기적신경세포조망이산생보호작용.
Objective To investigate if sevoflurane preconditioning attenuate neuronal apoptosis induced by ischemia-reperfusion.Methods Thirty-six male SD rats weiighing 250-300 g were randomly divided into three groups(n=12 each):control group(group C),ischemia-reperfusion group(group IR)(rats were established cerebral artery clamped and reperfusion model),sevonurane preconditioning group (group S)(rats were established cerebral artery clamped and reperfusion model after 1 h 2.4% sevoflurane preconditioning).Apoptosis nenrons were observed by Hematoxylin and Eosin(HE)staining and transmission electron microscope,TdT mediated Dutp nick end labeling(TUNEL)method wag used to count apoptosis neurons density,fresh ischemic brain tissue was taken out,while Caspase-3 zymogen and 20 000 segment were checked by Western blot.Results apoptosis neurons in group IR were more than ones in group S under HE staining and light microscope and transmission electron microscope,and apoptosis neurons density(cell number/0.1 mm~2)by TUNEL staining:group C,13.0±1.4;group IR,189.8±6.8;group S,110.5±4.3,the relative gray values of the contents of procaspase-3 and its 20 000 cleavage fragment were 16.72±3.0,76.1±3.4,51.2±3.1 and 8.2±2.3,59.0±6.3,31.2±5.4 respectively.Conclusions Sevoflurane pretreatment can protect neuron on ischemia-reperfusion injury by attenuating neuronal apoptosis in rats.
