CAJ | 학술논문

目的研究甘草酸对实验性梗阻性肾病大鼠是否具有保护作用。方法结扎大鼠单侧输尿管建立梗阻性肾病模型，分别于术后12 h，3、14、28和56 d处死实验动物，切取肾皮质部位组织，光、电镜观察肾间质病理形态学变化并进行有核细胞计数；免疫组织化学染色检测肾间质Ⅰ、Ⅲ型胶原蛋白表达，并用EI G综合图像分析系统对其表达进行半定量分析。结果模型显示肾间质呈进行性纤维化。术后12 h，甘草酸治疗组和预防治疗组与假手术组比较，肾间质有核细胞计数显著增多（P<0.05），与生理盐水组比较显著减少（P<0.01），但在甘草酸治疗组和预防治疗组差异无显著性（P>0.05）。半定量分析发现，肾间质Ⅰ型胶原蛋白的表达，在生理盐水组于术后12 h和假手术组比较差异有显著性（P<0.05）；术后3 d，甘草酸治疗组和预防治疗组肾间质Ⅰ型胶原蛋白的表达也高于假手术组（P<0.01），但显著低于生理盐水组（P<0.01），而甘草酸治疗组和预防治疗组差异无显著性（ P>0.05），Ⅲ型胶原蛋白的表达与Ⅰ型胶原蛋白的表达基本一致。结论甘草酸对大鼠梗阻性肾病具有一定的治疗作用，但无预防作用。
목적연구감초산대실험성경조성신병대서시부구유보호작용。방법결찰대서단측수뇨관건립경조성신병모형，분별우술후12 h，3、14、28화56 d처사실험동물，절취신피질부위조직，광、전경관찰신간질병리형태학변화병진행유핵세포계수；면역조직화학염색검측신간질Ⅰ、Ⅲ형효원단백표체，병용EI G종합도상분석계통대기표체진행반정량분석。결과모형현시신간질정진행성섬유화。술후12 h，감초산치료조화예방치료조여가수술조비교，신간질유핵세포계수현저증다（P<0.05），여생리염수조비교현저감소（P<0.01），단재감초산치료조화예방치료조차이무현저성（P>0.05）。반정량분석발현，신간질Ⅰ형효원단백적표체，재생리염수조우술후12 h화가수술조비교차이유현저성（P<0.05）；술후3 d，감초산치료조화예방치료조신간질Ⅰ형효원단백적표체야고우가수술조（P<0.01），단현저저우생리염수조（P<0.01），이감초산치료조화예방치료조차이무현저성（ P>0.05），Ⅲ형효원단백적표체여Ⅰ형효원단백적표체기본일치。결론감초산대대서경조성신병구유일정적치료작용，단무예방작용。
Objective　To investigate the protective role of glycyrrhizin on experimental obstructive nephropathy in rats. Methods 　The model of obstructive nephropathy was induced by unilateral uretera l ligation in rats and the animals were sacrificed at 12 h and 3,14,28,56 d resp ectively after operation. Specimens were taken from the cortex of kidney. In re nal stroma, routine morphological examinations and counts of karyocyte were made , the expression of collagen type Ⅰand type Ⅲ were also detected by immunohist ochemical staining,then, semi-quantified by EIG image analysis system. Results　 A progressive fibrosis was observed in renal stroma of the mod el. 12 h after operation, counts of karyocyte increased markedly i n renal stroma in groups of treatment and pretreatment with glycyrrhizin compar ed with that in sham-operation group（P<0.05）,and decreased distinctly compared with that in saline treated group（P<0.01）, but no significant di fference was found between treatment group and pretreatment group（P>0.05） ;3 d after operation, in group treated or pretreated with glycyrrhizin. The e xpression of collagen type Ⅰ increased markedly compared with that in sham-o peration group（P<0.01） in renal stroma, but decreased notably compare d with that in saline treated group（P<0.01）,while, there was not any diffe rence between treatment group and pretreatment group（P>0.05）. The expressi on of collagen type Ⅲ in renal stroma was almost the same as that of coll agen type Ⅰ. Conclusion　Glycyrrhizin has some therapeutical bu t no preventive effect to experimental obstructive nephropathy in rats.