CAJ | 학술논문

目的探讨在体外具有强大抗瘤活性的经肿瘤坏死因子-α(TNF-α)基因转导的肿瘤引流淋巴结细胞(DNL)在体内的抑瘤效果以及与平阳星(PYC)联合应用的可行性.方法将舌鳞癌细胞系Tca8113细胞注入SCID鼠背部皮下建立移植瘤模型,取经TNF-α基因转导的DNL细胞在肿瘤区局部注射,联合应用低剂量PYC,观察肿瘤牛长情况.至第8周以瘤重计算抑瘤率,并取瘤体标本经处理后作电镜和TUNEL检查,观察细胞凋亡情况.结果 TNF/DNL加重组白细胞介素-2(rIL-2)组和TNF/DNL加rIL-2加PYC组抑瘤率均较高,2组间差异有统计学意义P<0.05);TNF/DNL加rIL-2组超微电镜及TUNEL检测均见凋亡细胞,与对照组相比,凋亡指数(AI)较高(P<0.05).结论经TNF-A基因转导的DNL局部应用具有明显的抑瘤效果,与PYC联合应用抑瘤效果更大.诱导肿瘤细胞凋亡可能是TNF/DNL抗人舌鳞癌的重要机制之一.
목적 탐토재체외구유강대항류활성적경종류배사인자-α(TNF-α)기인전도적종류인류림파결세포(DNL)재체내적억류효과이급여평양성(PYC)연합응용적가행성.방법 장설린암세포계Tca8113세포주입SCID서배부피하건립이식류모형,취경TNF-α기인전도적DNL세포재종류구국부주사,연합응용저제량PYC,관찰종류우장정황.지제8주이류중계산억류솔,병취류체표본경처리후작전경화TUNEL검사,관찰세포조망정황.결과 TNF/DNL가중조백세포개소-2(rIL-2)조화TNF/DNL가rIL-2가PYC조억류솔균교고,2조간차이유통계학의의P<0.05);TNF/DNL가rIL-2조초미전경급TUNEL검측균견조망세포,여대조조상비,조망지수(AI)교고(P<0.05).결론 경TNF-A기인전도적DNL국부응용구유명현적억류효과,여PYC연합응용억류효과경대.유도종류세포조망가능시TNF/DNL항인설린암적중요궤제지일.
Objective To observe the in vivo inhibition effects of tumor necrosis factor-α (TNF-α) gene trans duced tumor drainage node of lymphocytes (DND from tongue cancer on SCID mice transplanted tumor. Methods 15 human tongue carcinoma models were established in SCID mice by subcutaneously injection of squamous cell car cinoma line Tca8113. TNF-a gene introduced DNL, combined with low dose Pinyancin (PYC), were locally injected into tumor site. The inhibition rate was determined by the weights at the 8th week after tumor dissection and fresh specimens were prepared and subject to histopathologic examination under transmission electron microscope, and in situ TUNEL was used to detect apoptosis. Results The TNF/DNL and rIL-2 group, and the TNF/DNL and rIL-2 and PYC group both exerted a strong inhibition effect on the implanted tumor. Treated tumors of the TNF/DNL and rIL-2 and PYC group were significantly reduced in comparison with those of the TNF/DNL and rIL-2 group (P< 0.05). The apoptosis of tumor in the TNF/DNL and rIL-2 group was evidenced based on transmission electron microscope and TUNEL analysis, and the apoptosis index was higher than that of control group (P<0.05). Conclusion Local injection of DNL modified with TNF-a gene, combined with low dose PYC, exert a synergistic antitumor effect. Apoptosis may be an important mechanism of squamous cell carcinoma killed by TNF/DNL.