CAJ | 학술논문

目的:研究慢性高眼压大鼠视网膜HSP70表达的变化及替普瑞酮的影响. 方法:Wistar大鼠70只随机分为3组:空白对照组10只;慢性高眼压模型组30只;慢性高眼压模型+GGA组30只.采用烧灼巩膜浅层静脉的方法制备大鼠慢性高眼压模型.模型成功后给予替普瑞酮800mg/(kg·d)每日灌胃.应用免疫组织化学方法观察应用及未应用替普瑞酮的慢性高眼压大鼠视网膜不同时点的差异及HSP70表达的变化.结果:平均眼压高于正常眼压40%的手术眼为造模成功.与正常对照组相比,慢性高眼压大鼠应用与未应用替普瑞酮者,视网膜均随着高眼压时间的延长逐渐出现形态学变化,于高眼压的21d和28d视网膜变薄,节细胞数量减少;在此过程中,HSP70表达增多.慢性高眼压大鼠视网膜应用替普瑞酮者与未应用提普瑞酮者相比,其形态学变化较小,而HSP70表达则明显增多,两者差异具有统计学意义(P<0.01).结论:替普瑞酮通过上调HSP70表达发挥对慢性高眼压视网膜的保护作用.
목적:연구만성고안압대서시망막HSP70표체적변화급체보서동적영향. 방법:Wistar대서70지수궤분위3조:공백대조조10지;만성고안압모형조30지;만성고안압모형+GGA조30지.채용소작공막천층정맥적방법제비대서만성고안압모형.모형성공후급여체보서동800mg/(kg·d)매일관위.응용면역조직화학방법관찰응용급미응용체보서동적만성고안압대서시망막불동시점적차이급HSP70표체적변화.결과:평균안압고우정상안압40%적수술안위조모성공.여정상대조조상비,만성고안압대서응용여미응용체보서동자,시망막균수착고안압시간적연장축점출현형태학변화,우고안압적21d화28d시망막변박,절세포수량감소;재차과정중,HSP70표체증다.만성고안압대서시망막응용체보서동자여미응용제보서동자상비,기형태학변화교소,이HSP70표체칙명현증다,량자차이구유통계학의의(P<0.01).결론:체보서동통과상조HSP70표체발휘대만성고안압시망막적보호작용.
AIM: To study the effects of geranylgeranylacetone(GGA) on the expression of heat shock protein70(HSP70) on retinal ganglion cells(RGC) in rats with chronic intraocular pressure(IOP) elevation.METHODS: Seventy Wistars were divided into blank control group(10 rats), chronic hypertension group(30 rats) and GGA group(30 rats). Chronic hypertension was created by cauterizing the superficial scleral veins. 800mg/(kg·d)GGA was given by oral daily after cauterization. Immunohistochemistry was used respectively to observe the changes of expression of HSP70 in the model rats and GGA interference rats at different time points during the course of chronic IOP elevation.RESULTS: The successful model was identified as the IOP over 40% of normal rats. The retinal thickness was significantly reduced in model group and model+ GGA group compared with normal rats from 21 days through 28 days after cauterization(P<0.05), and that of model rats was obviously decreased in comparison with model+ GGA rats(P<0.05). The number of ganglion cells was significantly decreased in model rats and model+ GGA rats compared with normal rats from 21 days and 28 days. The stronger expression intensity(IOD) value was seen for HSP70 in the model+ GGA rats by immunochemistry(P<0.01).CONCLUSION: Systemic administration of GGA protects retina from chronic IOP elevation by regulating the expression of HSP70.