CAJ | 학술논문

以从土壤中通过富集培养分离得到的2号菌株为对象,采用液体培养研究其在不同培养基、不同的碳、氮源、起始pH值、培养温度等条件下对孟加拉红和水溶性色浆的脱色效果.结果表明在以豆芽汁培养基和PDA培养基为基本培养基,蔗糖为碳源,硝酸铵为氮源,初始pH值为6.0～8.0时脱色效果最佳.根据菌株形态和ITS序列分析,初步鉴定该菌为黄曲霉.
이종토양중통과부집배양분리득도적2호균주위대상,채용액체배양연구기재불동배양기、불동적탄、담원、기시pH치、배양온도등조건하대맹가랍홍화수용성색장적탈색효과.결과표명재이두아즙배양기화PDA배양기위기본배양기,자당위탄원,초산안위담원,초시pH치위6.0～8.0시탈색효과최가.근거균주형태화ITS서렬분석,초보감정해균위황곡매.
The strain No. 2 which was isolated from the soil through enrichment culture was used as the experimental material. It was cultured in liquid medium to research decolorizing effect to Rose Bengal and soluble color paste under the different conditions of different media, carbon sources, nitrogen sources, initial pH values and culture temperatures. The results revealed that the optimum decolorizing conditions were using bean juice medium and PDA medium as the minimal medium, sucrose as the carbon source, and ammonium nitrate as the nitrogen source, initial pH 6.0-8.0. In addition, the strain was primarily identified as Aspergillus flavus according to its morphous and ITS sequence analysis.