青岛海洋大学学报(自然科学版)
青島海洋大學學報(自然科學版)
청도해양대학학보(자연과학판)
JOURNAL OF OCEAN UNIVERSITY OF QINGDAO
2000年
1期
81-86
,共6页
叶眉%薛长湖%方煜%孙方强
葉眉%薛長湖%方煜%孫方彊
협미%설장호%방욱%손방강
chitosan%papain%depolymerization
One kind of papain was used to depolymerize chitosan in this paper. Four factors of reactions were studied including concentration of enzyme, pH, temperature and time. The result indicated that temperature influenced the depolymerization of chitosan efficiently, while time had relatively little effect on it with pH from 3.0 to 6.0, temperature from 30℃ to 60℃, and time from 2 to 8 hours. The data revealed that the enzymatic process did not obey a simple kinetic model; chitosan with average molecular weights in the range (5-10)×105 could be easily depolymerized to highly polydisperse chitosans under conditions such as pH 4.0, 40℃, 8 hrs, and papain 1.5 mg/mL.
