中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2008年
6期
534-537
,共4页
张亚兴%王志刚%许川山%张群霞%张茂惠%谢昭鹏
張亞興%王誌剛%許川山%張群霞%張茂惠%謝昭鵬
장아흥%왕지강%허천산%장군하%장무혜%사소붕
超声检查%微气泡%肌,骨骼%新生血管化,生理性
超聲檢查%微氣泡%肌,骨骼%新生血管化,生理性
초성검사%미기포%기,골격%신생혈관화,생이성
Ultrasonography%Microbubbles%Muscle,skeletal%Neovascularization,physiologic
目的 探讨超声破坏微泡促进大鼠骨骼肌血管新生的时间-效应关系.方法 将48只SD大鼠随机分为4组(超声破坏微泡组、单纯超声组、单纯微泡组、对照组),超声破坏微泡组经尾静脉输入自制的脂质全氟丙烷微泡造影剂0.5 ml,同时用1.0 MHz、2.0 W/cm2的超声在其大腿骨骼肌局部作用3min;单纯超声组仅在骨骼肌局部用同等的超声能量作用;单纯微泡组仅由尾静脉输入脂质全氟丙烷微泡造影剂0.5 ml;对照组不作任何处理.实验结束后的第3、7、10、14、21、28 d,各组分别取两只大鼠处死,取局部骨骼肌行石蜡切片HE染色观察显微结构的变化,CD34免疫组化计数微血管密度(MVD),酶联免疫吸附试验(ELISA)定量评价血管内皮生长因子(VEGF)的表达.结果 超声破微泡组有较多的血管新生;单纯超声组新生血管较少;单纯微泡组和对照组几乎无血管新生.随着时间的变化,超声破坏微泡组的MVD值和VEGF表达在第10 d达到高峰;单纯超声组的高峰出现在第14 d.结论 超声破坏微泡可刺激骨骼肌中内源性VEGF较快、较多地分泌,从而更好地促进新生血管生成.
目的 探討超聲破壞微泡促進大鼠骨骼肌血管新生的時間-效應關繫.方法 將48隻SD大鼠隨機分為4組(超聲破壞微泡組、單純超聲組、單純微泡組、對照組),超聲破壞微泡組經尾靜脈輸入自製的脂質全氟丙烷微泡造影劑0.5 ml,同時用1.0 MHz、2.0 W/cm2的超聲在其大腿骨骼肌跼部作用3min;單純超聲組僅在骨骼肌跼部用同等的超聲能量作用;單純微泡組僅由尾靜脈輸入脂質全氟丙烷微泡造影劑0.5 ml;對照組不作任何處理.實驗結束後的第3、7、10、14、21、28 d,各組分彆取兩隻大鼠處死,取跼部骨骼肌行石蠟切片HE染色觀察顯微結構的變化,CD34免疫組化計數微血管密度(MVD),酶聯免疫吸附試驗(ELISA)定量評價血管內皮生長因子(VEGF)的錶達.結果 超聲破微泡組有較多的血管新生;單純超聲組新生血管較少;單純微泡組和對照組幾乎無血管新生.隨著時間的變化,超聲破壞微泡組的MVD值和VEGF錶達在第10 d達到高峰;單純超聲組的高峰齣現在第14 d.結論 超聲破壞微泡可刺激骨骼肌中內源性VEGF較快、較多地分泌,從而更好地促進新生血管生成.
목적 탐토초성파배미포촉진대서골격기혈관신생적시간-효응관계.방법 장48지SD대서수궤분위4조(초성파배미포조、단순초성조、단순미포조、대조조),초성파배미포조경미정맥수입자제적지질전불병완미포조영제0.5 ml,동시용1.0 MHz、2.0 W/cm2적초성재기대퇴골격기국부작용3min;단순초성조부재골격기국부용동등적초성능량작용;단순미포조부유미정맥수입지질전불병완미포조영제0.5 ml;대조조불작임하처리.실험결속후적제3、7、10、14、21、28 d,각조분별취량지대서처사,취국부골격기행석사절편HE염색관찰현미결구적변화,CD34면역조화계수미혈관밀도(MVD),매련면역흡부시험(ELISA)정량평개혈관내피생장인자(VEGF)적표체.결과 초성파미포조유교다적혈관신생;단순초성조신생혈관교소;단순미포조화대조조궤호무혈관신생.수착시간적변화,초성파배미포조적MVD치화VEGF표체재제10 d체도고봉;단순초성조적고봉출현재제14 d.결론 초성파배미포가자격골격기중내원성VEGF교쾌、교다지분비,종이경호지촉진신생혈관생성.
Objective To investigate the time-effect relationship of angiogenesis induced by ultrasound.mediated microbubble destruction in the skeletal muscle of rats.Methods Forty-eight healthy SD rats were divided into 4 groups:ultrasound-mediated microbubble destruction group,ultrasound only group,microbubble only group and control group.In ultrasound-mediated mierobubble destruction group, microbubbles were inj ected by vein at a dose of 0.5 ml and the target skeletal muscle was radiated at 2.0W/cm2.In ultrasound only group,the target skeletal muscle was radiated at 2.0 W/cm2.In microbubble only group.microbubbles were injected by vein at a dose of 0.5 ml.The control rats were without ultrasound radiation and microbubbles.On the 3rd,7th,10th,14th,21st and 28th day after the ultrasound radiation,two rats in each group were sacrificed and the target skeletal muscle was harvested for HE staining to observe the microstructure of tissue,immunohistochemistry staining was used to count the microvessel density (MVD),enzyme 1inked inmmunosorbent assay(ELISA)was used to detect the expression of vascular endothelial grouth factor(VEGF).Results Angiogenesis was significant in ultrasound-mediated mierobubhle destruction group,but a little in ultrasound only group.There was not any angiogenesis in either microbubble only group or control group.MVD and VEGF expression of ultrasound-mediated microbubble destruction group arrived at a peak on the 1Oth day as well as on the 14th day of ultrasound group.Conclusions Ultrasound-mediated microbubble destruction can facilliate the endogenous secretion of VEGF quickly and more,and accelerate the angiogenesis in the skeletal muscle.