中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
2期
129-132
,共4页
彭成为%余开峰%杨镭镭%张丽%曹书芬%王清秀%姚尚龙
彭成為%餘開峰%楊鐳鐳%張麗%曹書芬%王清秀%姚尚龍
팽성위%여개봉%양뢰뢰%장려%조서분%왕청수%요상룡
神经生长因子%注射,脊髓%转染%神经痛%P物质%降钙素基因相关肽
神經生長因子%註射,脊髓%轉染%神經痛%P物質%降鈣素基因相關肽
신경생장인자%주사,척수%전염%신경통%P물질%강개소기인상관태
Nerve growth factor%Injections,spinal%Transfection%Neuropathic pain%Substance P%Calcitonin gene-related peptide
目的 探讨人神经生长因子β(hNGFβ)基因转染对神经病理性痛大鼠脊髓背角降钙素基因相关肽(CGRP)和P物质(SP)含量的影响.方法 雄性SD大鼠48只,体重200～250 g,随机分为3组(n=16):假手术组(Ⅰ组)假手术后立即鞘内注射人工脑脊液;Ⅱ组和Ⅲ组制备坐骨神经慢性压迫性损伤(CCI)模型,术后分别立即鞘内注射人工脑脊液或重组腺病毒介导入神经生长因子β(Ad-hNGFβ)基因.于术前1 d、转染后28 d内每4天测定热痛阈、机械痛阈及行为学评分.每组分别于转染后4、7、14及28 d各处死4只大鼠,取脊髓组织,采用免疫组织化学法测定SP和CGRP含量.结果 与Ⅰ组比较,Ⅱ组和Ⅲ组行为学评分升高,机械痛阈及热痛阈均降低(P<0.01);与Ⅱ组比较,Ⅲ组行为学评分及机械痛阈差异无统计学意义(P>0.05),转染后8～24 d热痛阈升高(P<0.05).术后Ⅱ组和Ⅲ组术侧脊髓背角SP及CGRP含量明显高于Ⅰ组,术后7～28 dm组术侧脊髓背角SP及CGRP含量明显低于Ⅱ组(P<0.05或0.01).结论 Ad-hNGFβ基因转染可能通过降低脊髓背角SP及CGRP含量减轻神经病理性痛大鼠的热痛觉过敏.
目的 探討人神經生長因子β(hNGFβ)基因轉染對神經病理性痛大鼠脊髓揹角降鈣素基因相關肽(CGRP)和P物質(SP)含量的影響.方法 雄性SD大鼠48隻,體重200～250 g,隨機分為3組(n=16):假手術組(Ⅰ組)假手術後立即鞘內註射人工腦脊液;Ⅱ組和Ⅲ組製備坐骨神經慢性壓迫性損傷(CCI)模型,術後分彆立即鞘內註射人工腦脊液或重組腺病毒介導入神經生長因子β(Ad-hNGFβ)基因.于術前1 d、轉染後28 d內每4天測定熱痛閾、機械痛閾及行為學評分.每組分彆于轉染後4、7、14及28 d各處死4隻大鼠,取脊髓組織,採用免疫組織化學法測定SP和CGRP含量.結果 與Ⅰ組比較,Ⅱ組和Ⅲ組行為學評分升高,機械痛閾及熱痛閾均降低(P<0.01);與Ⅱ組比較,Ⅲ組行為學評分及機械痛閾差異無統計學意義(P>0.05),轉染後8～24 d熱痛閾升高(P<0.05).術後Ⅱ組和Ⅲ組術側脊髓揹角SP及CGRP含量明顯高于Ⅰ組,術後7～28 dm組術側脊髓揹角SP及CGRP含量明顯低于Ⅱ組(P<0.05或0.01).結論 Ad-hNGFβ基因轉染可能通過降低脊髓揹角SP及CGRP含量減輕神經病理性痛大鼠的熱痛覺過敏.
목적 탐토인신경생장인자β(hNGFβ)기인전염대신경병이성통대서척수배각강개소기인상관태(CGRP)화P물질(SP)함량적영향.방법 웅성SD대서48지,체중200～250 g,수궤분위3조(n=16):가수술조(Ⅰ조)가수술후립즉초내주사인공뇌척액;Ⅱ조화Ⅲ조제비좌골신경만성압박성손상(CCI)모형,술후분별립즉초내주사인공뇌척액혹중조선병독개도입신경생장인자β(Ad-hNGFβ)기인.우술전1 d、전염후28 d내매4천측정열통역、궤계통역급행위학평분.매조분별우전염후4、7、14급28 d각처사4지대서,취척수조직,채용면역조직화학법측정SP화CGRP함량.결과 여Ⅰ조비교,Ⅱ조화Ⅲ조행위학평분승고,궤계통역급열통역균강저(P<0.01);여Ⅱ조비교,Ⅲ조행위학평분급궤계통역차이무통계학의의(P>0.05),전염후8～24 d열통역승고(P<0.05).술후Ⅱ조화Ⅲ조술측척수배각SP급CGRP함량명현고우Ⅰ조,술후7～28 dm조술측척수배각SP급CGRP함량명현저우Ⅱ조(P<0.05혹0.01).결론 Ad-hNGFβ기인전염가능통과강저척수배각SP급CGRP함량감경신경병이성통대서적열통각과민.
Objective To investigate the effects of adenovirus containing human beta-nerve growth factor (Ad-hNGFβ) gene on substance P (SP) and calcitonin gene-related peptide (CGRP) content of the spinal cord in a rat model of neuropathic pain by chronic constrictive injury (CCI). Methods Forty-eight male SD rats weighing 200-250 g were randomly divided into 3 groups (n=16 each) : group Ⅰ sham operation; group Ⅱ CCI and group Ⅲ CCI + Ad-hNGFβ gene IT. The animals were anesthetized with intraperitoneal choral hydrate 300-350 mg/kg. The right sciatic nerve was exposed and 4 ligatures were placed on the sciatic nerve at 1-2 nun intervals as described by Bennet and Xie[5]. In sham operation group, right sciatic nerve was exposed but not ligated. In group Ⅰ and Ⅱ artificial cerebrnspinal fluid was injected IT instead of Ad-hNGFβ gene. The behavior score and the paw-withdrawal latency (PWL) to radiant heat and mechanical stimulus were measured one day before operation and every 4 days within the 28 days after gene transfection. Four animals were killed at 4, 7, 14 and 28 day after IT gene transfection in each group and lumbar segment (L4-6 ) of the spinal cord was removed for determination of SP and CGRP content by immunohistochemistry. Results The behavior scores were significantly higher and PWL to radiant heat and mechanical stimulus were significantly lower in group Ⅱ and Ⅲ than in group Ⅰ. There was no significant difference in the behavior score and PWL to mechanical stimulus between group Ⅱ and Ⅲ while the PWL to radiant heat was significantly higher in group Ⅲ than in group Ⅱ. After operation SP and CGRP content were significantly higher in group Ⅱ and group Ⅲ than in group Ⅰ , and significangly lower in group Ⅲ than in group Ⅱ 7-28 days after operation. Conclusion The recomhinant Ad-hNGFβ gene transfection can attenuate heat hyperalgesia by reducing SP and CGRP content of the spinal cord in a rat model of neuropathic pain induced by CCI.
