中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
10期
730-734
,共5页
贾聪聪%林林%刘妮娅%张小晶%张佳佳%杨新军%黄陈平
賈聰聰%林林%劉妮婭%張小晶%張佳佳%楊新軍%黃陳平
가총총%림림%류니아%장소정%장가가%양신군%황진평
铅%成纤维细胞生长因子3%胚胎发育%基因表达
鉛%成纖維細胞生長因子3%胚胎髮育%基因錶達
연%성섬유세포생장인자3%배태발육%기인표체
Lead%Fibroblast growth factor 3%Embryonic development%Gene expression
目的 研究铅暴露对斑马鱼胚胎发育过程Fgf3基因表达的影响,以探讨铅的胚胎发育毒性机制.方法 野生型AB品系斑马鱼胚胎暴露醋酸铅浓度分别为0、0.1、0.5、2.5和12.5 μmol/L,提取各组8、12、16、24、36、48及72 hpf(受精后小时)斑马鱼胚胎总RNA,实时荧光PCR检测Fgf3基因定量表达；制备Fgf3基因RNA探针,整胚原位杂交方法检测Fgf3基因空间性表达.结果 在胚胎发育全程中,各组Fgf3表达高峰均在12 hpf(与其他时段比较,P＜0.01),随着铅暴露浓度的升高,Fgf3 mRNA 平均表达量也逐渐增加,以对照组Fgf3 mRNA表达量为基准,各铅暴露组(0.1、0.5、2.5和12.5 μmol/L)相对表达量分别为1.02±0.24、1.05±0.26、1.22±0.46和1.25±0.38,其中2.5和12.5μmol/L铅浓度组Fgf3表达量均显著高于对照组(P＜0.05)；整胚原位杂交结果显示Fgf3主要表达部位是胚胎早期的头、尾部和胚胎中后期的中脑、鳍芽及咽弓部,在胚胎发育至12 hpf时Fgf3阳性杂交信号区域及强度最为明显,但各铅暴露组与对照比较,在Fgf3表达部位及强度上未见明显差异.结论 胚胎期铅暴露能引起Fgf3表达水平上调,改变了胚胎发育过程Fgf3正常表达规律,其可能与铅胚胎发育毒性有关.
目的 研究鉛暴露對斑馬魚胚胎髮育過程Fgf3基因錶達的影響,以探討鉛的胚胎髮育毒性機製.方法 野生型AB品繫斑馬魚胚胎暴露醋痠鉛濃度分彆為0、0.1、0.5、2.5和12.5 μmol/L,提取各組8、12、16、24、36、48及72 hpf(受精後小時)斑馬魚胚胎總RNA,實時熒光PCR檢測Fgf3基因定量錶達；製備Fgf3基因RNA探針,整胚原位雜交方法檢測Fgf3基因空間性錶達.結果 在胚胎髮育全程中,各組Fgf3錶達高峰均在12 hpf(與其他時段比較,P＜0.01),隨著鉛暴露濃度的升高,Fgf3 mRNA 平均錶達量也逐漸增加,以對照組Fgf3 mRNA錶達量為基準,各鉛暴露組(0.1、0.5、2.5和12.5 μmol/L)相對錶達量分彆為1.02±0.24、1.05±0.26、1.22±0.46和1.25±0.38,其中2.5和12.5μmol/L鉛濃度組Fgf3錶達量均顯著高于對照組(P＜0.05)；整胚原位雜交結果顯示Fgf3主要錶達部位是胚胎早期的頭、尾部和胚胎中後期的中腦、鰭芽及嚥弓部,在胚胎髮育至12 hpf時Fgf3暘性雜交信號區域及彊度最為明顯,但各鉛暴露組與對照比較,在Fgf3錶達部位及彊度上未見明顯差異.結論 胚胎期鉛暴露能引起Fgf3錶達水平上調,改變瞭胚胎髮育過程Fgf3正常錶達規律,其可能與鉛胚胎髮育毒性有關.
목적 연구연폭로대반마어배태발육과정Fgf3기인표체적영향,이탐토연적배태발육독성궤제.방법 야생형AB품계반마어배태폭로작산연농도분별위0、0.1、0.5、2.5화12.5 μmol/L,제취각조8、12、16、24、36、48급72 hpf(수정후소시)반마어배태총RNA,실시형광PCR검측Fgf3기인정량표체；제비Fgf3기인RNA탐침,정배원위잡교방법검측Fgf3기인공간성표체.결과 재배태발육전정중,각조Fgf3표체고봉균재12 hpf(여기타시단비교,P＜0.01),수착연폭로농도적승고,Fgf3 mRNA 평균표체량야축점증가,이대조조Fgf3 mRNA표체량위기준,각연폭로조(0.1、0.5、2.5화12.5 μmol/L)상대표체량분별위1.02±0.24、1.05±0.26、1.22±0.46화1.25±0.38,기중2.5화12.5μmol/L연농도조Fgf3표체량균현저고우대조조(P＜0.05)；정배원위잡교결과현시Fgf3주요표체부위시배태조기적두、미부화배태중후기적중뇌、기아급인궁부,재배태발육지12 hpf시Fgf3양성잡교신호구역급강도최위명현,단각연폭로조여대조비교,재Fgf3표체부위급강도상미견명현차이.결론 배태기연폭로능인기Fgf3표체수평상조,개변료배태발육과정Fgf3정상표체규률,기가능여연배태발육독성유관.
Objective To investigate the effect of lead exposure on the gene expression of fibroblast growth factor 3 (Fgf3) in zebrafish embryonic development and the mechanism of lead-induced embryonic developmental toxicity.Methods The embryos of zebrafish (wild types A and B) were exposed to lead acetate (PbAc) at the doses of 0,0.1,0.5,2.5,and 12.5 μmol/L separately.Total RNA was extracted from each treatment group of zebrafish embryos at 8,12,16,24,36,48,and 72 hours post fertilization (hpf).The total mRNA expression of Fgf3 was measured by real-time quantitative PCR.The spatial expression of Fgf3 in zebrafish embryos was determined by whole-mount in situ hybridization using synthesized Fgf3 RNA probe.Results The mRNA expression of Fgf3 in each group peaked at 12 hpf (P＜0.01).With the increase in PbAc concentration,the mRNA expression of Fgf3 rose.Compared with the mRNA expression level of Fgf3 in the control group,the relative mRNA expression levels of Fgf3 in the 0.1,0.5,2.5,and 12.5 μmol/L PbAc exposure groups were 1.02±0.24,1.05±0.26,1.22±0.46,and 1.25±0.38,respectively,and the 2.5 and 12.5 μ mol/L PbAc exposure groups showed significantly higher Fgf3 expression than the control group (P＜0.05).The whole-mount in situ hybridization results showed that Fgf3 expression occurred mainly in the head and tail in the early stage of embryonic development and in the midbrain,fin bud,and pharyngeal arch in the middle/late stage of embryonic development; there were the most significant regions and intensities of positive hybridization signals at 12 hpf; but no significant differeuces were found between the control group and exposure groups in the location and intensity of Fgf3 expression Conclusion Lead exposure can result in the upregulation of Fgf3 expression in zebrafish embryonic development,which might contribute to lead-induced embryonic developmental toxicity.
