CAJ | 학술논문

目的探讨L-选择素P213S基因多态性在甘肃汉族、东乡族人群中的分布及其与2型糖尿病的关系.方法 2006至2008年入选汉族2型糖尿病患者143例和正常对照者152名,东乡族2型糖尿病患者116例和正常对照者126名,应用聚合酶链反应-高温连接酶检测反应检测L-选择素P213S基因型,采用氧化酶法或放免法测定血糖、胰岛素及血脂水平.采用卡方检验进行统计学分析.结果 L-选择素基因型PP、PS和SS频率在汉族2型糖尿病患者为55.9%、37.8%及6.3%,在汉族正常对照者为41.4%、50.7%及7.9%,在东乡族2型糖尿病患者为44.8%、48.3%及6.9%,在东乡族正常对照者为38.1%、44.4%及17.5%.等位基因P、S频率在汉族2型糖尿病患者为74.8%及25.2%,在汉族正常对照者为66.8%及33.2%,在东乡族2型糖尿病患者为69.0%及31.0%,在东乡族正常对照者为60.3%及39.7%(均P<0.05).汉族人群P等位基因携带者2型糖尿病患病风险是S等位基因携带者的1.479倍,东乡族人群P等位基因携带者2型糖尿病患病风险是S等位基因携带者的1.462倍.结论 L-选择素P213S基因多态性与2型糖尿病发病相关,P等位基因可能是罹患2型糖尿病的危险因素之一.
목적 탐토L-선택소P213S기인다태성재감숙한족、동향족인군중적분포급기여2형당뇨병적관계.방법 2006지2008년입선한족2형당뇨병환자143례화정상대조자152명,동향족2형당뇨병환자116례화정상대조자126명,응용취합매련반응-고온련접매검측반응검측L-선택소P213S기인형,채용양화매법혹방면법측정혈당、이도소급혈지수평.채용잡방검험진행통계학분석.결과 L-선택소기인형PP、PS화SS빈솔재한족2형당뇨병환자위55.9%、37.8%급6.3%,재한족정상대조자위41.4%、50.7%급7.9%,재동향족2형당뇨병환자위44.8%、48.3%급6.9%,재동향족정상대조자위38.1%、44.4%급17.5%.등위기인P、S빈솔재한족2형당뇨병환자위74.8%급25.2%,재한족정상대조자위66.8%급33.2%,재동향족2형당뇨병환자위69.0%급31.0%,재동향족정상대조자위60.3%급39.7%(균P<0.05).한족인군P등위기인휴대자2형당뇨병환병풍험시S등위기인휴대자적1.479배,동향족인군P등위기인휴대자2형당뇨병환병풍험시S등위기인휴대자적1.462배.결론 L-선택소P213S기인다태성여2형당뇨병발병상관,P등위기인가능시리환2형당뇨병적위험인소지일.
Objective To investigate P213S gene polymorphism of L-selectin in people of Han and Dongxiang nationalities in Gansu Province and its association with type 2 diabetes. Methods A total of 143 patients with type 2 diabetes and 152 healthy controls of Han nationality and 116 patients with type 2 diabetes and 126 healthy controls of Dongxiang nationality were enrolled in this study during 2006 and 2008.Genotype of P213S was determined by polymerase chain reaction-ligation diction reaction. Plasma glucose,insulin, and lipid levels were measured by biochemical technique. χ2 test was used for data analysis.ResultS L-selectin genotype frequencies of PP, PS, and SS were 55.9%, 37.8%, and 6. 3% in type 2 diabetic patients of Han nationality; 41.4%, 50. 7%, and 7.9% in normal controls of Han nationality;44.8%, 48.3%, and 6.9% in type 2 diabetic patients of Dongxiang nationality; 38. 1%, 44.4%, and 17.5% in normal controls of Dongxiang nationality. Allele frequencies of P or S were 74. 8% and 25.2% in type 2 diabetic patients of Han nationality, 66. 8% and 33.2% in normal controls of Han nationality,69.0% and 31.0% in type 2 diabetic patients of Dongxiang nationality, 60. 3% and 39. 7% in normal controls of Dongxiang nationality (all P <0.05). The risk of type 2 diabetes of P allele carriers was 1.479 or 1.462 times of S allele carriers in Han or Dongxiang nationality. Conclusion L-selectin P213S polymorphism might be associated with type 2 diabetes, and P allele seems to be a risk factor of the disease.