CAJ | 학술논문

目的观察给予双歧杆菌及其微囊化制剂预先处理对大鼠失血性休克模型肠道屏障和细菌移位的影响.方法 SD大鼠每天经口给予磷酸盐缓冲液(PBS)盐水、双歧杆菌(1×109cfu)或相同数量的微囊化双歧杆菌,喂养7 d后分别行90 min的休克或假休克,液体复苏后3 h剖腹取样行肠道菌群分析、细菌移位和回肠病理学检测.结果 3种不同处理的失血性休克大鼠组间总失血量相似.双歧杆菌预先处理可降低失血性休克大鼠盲肠内总需氧菌数量,微囊化双歧杆菌可降低更加明显.盲肠内总厌氧菌和双歧杆菌数量,双歧杆菌组增高,双歧杆菌微囊组更高.休克大鼠细菌移位到肠系膜淋巴结的发生率,双歧杆菌微囊组较PBS组降低;总需氧菌移位幅度,双歧杆菌休克大鼠的较PBS组降低,微囊组休克大鼠的细菌移位幅度较双歧杆菌组进一步降低;脾的总厌氧菌和双歧杆菌移位幅度却较PBS休克组显著性降低.双歧杆菌预先处理后休克大鼠的的绒毛损伤百分率,较PBS休克组明显减轻.结论双歧杆菌可保护失血性休克大鼠的肠道屏障、减轻细菌移位,微囊化双歧杆菌可进一步增强这一效果.
목적 관찰급여쌍기간균급기미낭화제제예선처리대대서실혈성휴극모형장도병장화세균이위적영향.방법 SD대서매천경구급여린산염완충액(PBS)염수、쌍기간균(1×109cfu)혹상동수량적미낭화쌍기간균,위양7 d후분별행90 min적휴극혹가휴극,액체복소후3 h부복취양행장도균군분석、세균이위화회장병이학검측.결과 3충불동처리적실혈성휴극대서조간총실혈량상사.쌍기간균예선처리가강저실혈성휴극대서맹장내총수양균수량,미낭화쌍기간균가강저경가명현.맹장내총염양균화쌍기간균수량,쌍기간균조증고,쌍기간균미낭조경고.휴극대서세균이위도장계막림파결적발생솔,쌍기간균미낭조교PBS조강저;총수양균이위폭도,쌍기간균휴극대서적교PBS조강저,미낭조휴극대서적세균이위폭도교쌍기간균조진일보강저;비적총염양균화쌍기간균이위폭도각교PBS휴극조현저성강저.쌍기간균예선처리후휴극대서적적융모손상백분솔,교PBS휴극조명현감경.결론 쌍기간균가보호실혈성휴극대서적장도병장、감경세균이위,미낭화쌍기간균가진일보증강저일효과.
Objective To investigate the effects of micro-encapsulated bifidobacteria on gut harrier and bacterial translocation after hemorrhagic shock and resuscitation. Methods Sprague-Dawley rats were divided into 6 groups : PBS + sham shock group fed with PBS for 7 days and then undergoing sham shock, bifidobacteria + sham shock group fed with bifidobacteria (109cfu/d) for 7 days and then undergoing sham shock, micro-encapsulated bifidobacteria + sham shock group, fed with micro-encapsulated bifidobacteria (109 cfu/d) for 7 days and then undergoing sham shock, PBS + hemorrhagic shock group fed with PBS for 7 days and then undergoing hemorrhagic shock, bifidobacteria + shock group fed with bifidobacteria for 7 days and then undergoing hemorrhagic shock, and micro-encapsulated bifidobacteria + shock group, fed with micro-encapsulated bifidobacteria for 7 days and then undergoing hemorrhagic shock. Three hours after resuscitation laparotomy was performed, distal cecum was resected to undergo bacteriological analysis of the cecal content, mesenteric lymph nodes (MLNs), a liver lobe, and the middle part of spleen were resected to undergo bacterial culture for bacterial translocation, and the terminal ileum was resected to observe the villous damage. Results There was no significant difference in the amount of blood loss among the 3 hemorrhagic shock groups. The amounts of aerobes in cecum of the bifidobactefia + shock and micro-encapsulated bifidobacteria + shock groups, especially that of the latter group, were significantly lower than that of the PBS + shock group. The amounts of anaerobes and the amounts of bifidobacteria in cecum of the bifidobacteria + shock group and micro-encapsulated bifidobacteria + shock group, especially those of the latter group, were significantly higher than those of the PBS + shock group. No bacterial translocation to liver was observed in all groups. The magnitudes of total aerobes translocation in spleen of the bifidobacteria +shock and encapsulated bifidobaeteria + shock groups were significantly lower than that of the PBS + shoc group, however, there were not significant differences in the translocation in the MEN of total aerobes ad bifidobacteria among different groups. The percentage of ileal villous damage of the bifidobacteria + shock and encapsulated bifidobacteria + shock groups were significantly lower than that of the PBS + shock group. Conclusion Bifidobacteria effectively protects the gut barrier, reduces bacterial translocation from the gut after hemorrhagic shock and resuscitation. And micro-encapsulated Bifidobacteria can enhance those effects further.