CAJ | 학술논문

目的探讨Beclin 1和PTEN蛋白在胃癌发生过程中的意义及其对预后的影响.方法应用组织芯片和免疫组织化学法检测199例胃癌及相应癌旁正常组织中Beclin 1和PTEN蛋白的表达,分析其与胃癌的关系；用Western印迹方法检测15例新鲜胃癌及相应癌旁组织中Beclin1和PTEN蛋白的表达率.所有标本均来自上海长征医院.结果免疫组织化学法检测提示癌组织中Beelin 1和PTEN蛋白阳性表达率分别为47.2％(94/199)和55.8％(111/199),均低于相应癌旁组织的表达率[94.5％(188/199)和92.5％(184/199),P＜0.01].胃癌组织中Beelin 1和PTEN蛋白的低表达均与性别、分化程度、肿瘤浸润深度、淋巴结转移以及疾病分期有关(P＜0.05).胃癌组织中Beclin 1和PTEN的表达呈正相关(r=0.680,P＜0.01).生存分析表明Beclin 1和PTEN均是判断胃癌患者预后的独立因素.Beclin 1阳性患者的5年生存率为67.0％(63/94),阴性者为33.3％ (35/105)；PTEN阳性患者的5年生存率为71.2％ (79/111),阴性者为21.6％(19/88)(P值均＜0.01).Western印迹方法检测结果提示胃癌标本中Beclin 1和PTEN蛋白含量明显低于相应癌旁正常组织(P值均＜0.01).结论 Beclin 1和PTEN的异常表达可能和胃癌的发生发展相关.
목적 탐토Beclin 1화PTEN단백재위암발생과정중적의의급기대예후적영향.방법 응용조직심편화면역조직화학법검측199례위암급상응암방정상조직중Beclin 1화PTEN단백적표체,분석기여위암적관계；용Western인적방법검측15례신선위암급상응암방조직중Beclin1화PTEN단백적표체솔.소유표본균래자상해장정의원.결과 면역조직화학법검측제시암조직중Beelin 1화PTEN단백양성표체솔분별위47.2％(94/199)화55.8％(111/199),균저우상응암방조직적표체솔[94.5％(188/199)화92.5％(184/199),P＜0.01].위암조직중Beelin 1화PTEN단백적저표체균여성별、분화정도、종류침윤심도、림파결전이이급질병분기유관(P＜0.05).위암조직중Beclin 1화PTEN적표체정정상관(r=0.680,P＜0.01).생존분석표명Beclin 1화PTEN균시판단위암환자예후적독립인소.Beclin 1양성환자적5년생존솔위67.0％(63/94),음성자위33.3％ (35/105)；PTEN양성환자적5년생존솔위71.2％ (79/111),음성자위21.6％(19/88)(P치균＜0.01).Western인적방법검측결과제시위암표본중Beclin 1화PTEN단백함량명현저우상응암방정상조직(P치균＜0.01).결론 Beclin 1화PTEN적이상표체가능화위암적발생발전상관.
Objective To explore the role of Beclin 1 and PTEN in gastric caicinoma genesis and the effects on prognosis.Methods The expression of Beclin 1 and PTEN in 199 gastric caicinoma specimens and corresponding adjacent noncancerous tissues were examined by tissue microarrays and immunohistochemistary,and the relation with gastric cancer was analyzed.The rate of Beclin 1 and PTEN expression in 15 fresh gastric carcinoma samples and corresponding adjacent noncancerous tissues were detected by Western blot.All the samples were from Changzheng Hospital.Results The Results of immunohistochemistary showed that the rates of Beclin 1 and PTEN positive expression in cancinoma tissues were 47.2％ (94/199) and 55.8％ (111/199),both were lower than that of adjacent noncancinoma tissues (94.5％,188/199 and 92.5％,184/199; P ＜ 0.01).The lower expression of Beclin 1 and PTEN in gastric carcinoma were associated with gender,differentiation degree,depth of tumor invasion,lymph node metastases and disease stage(P＜0.05).There was a positive correlation between Beclin 1 and PTEN expression in gastric carcinoma tissues (r =0.680,P＜0.01). The survival analysis indicated that Beclin 1 and PTEN were independent factors in determining the prognosis of gastric cancinoma patients.The 5-year survival rate of Beclin 1 positive patients was 67.0％ (63/94),and of negative patients was 33.3％ (35/105).The 5-year survival rate of PTEN positive patients was 71.2％ (79/111),and of negative patients was 21.6％ (19/88) ( all P＜0.001).The Results of Western blot indicated that the expression of Beclin 1 and PTEN in gastric carcinoma tissues were significantly lower than that in the adjacent noncarcinoma tissues ( all P＜0.001).Conclusion The abnormal expression of Beclin 1 and PTEN may be related to carcinogenesis and the development of gastric carcinoma.