中国人民解放军军医大学学报
中國人民解放軍軍醫大學學報
중국인민해방군군의대학학보
JOURNAL OF MEDICAL COLLEGES OF PLA
2003年
2期
73-76
,共4页
江渝%刘红%彭家和%叶治家%何凤田%董燕麟%刘秉文
江渝%劉紅%彭傢和%葉治傢%何鳳田%董燕麟%劉秉文
강투%류홍%팽가화%협치가%하봉전%동연린%류병문
high density lipoprotein%human arterial smooth muscle cell%oxidative modification%cell culture
Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by cultured human arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected, and native HDL (N-HDL) served as control. Oxidative modification of HDL was identified by using agarose gel electrophoresis. Absorbances of conjugated diene (CD) and lipid hydroperoxide (LOOH) were measured with ultraviolet spectrophotometry at 234 and 560 nm respectively, and fluorescence intensity of thiobarbuturic acid reaction substance (TBARS) with fluorescence spectrophotometry at 550 nm emission wavelength with excitation at 515 nm. Results: In comparison with N-HDL, the electrophoretic mobility of SMCs-cocultured HDL was increased, and the contents of CD, LOOH and TBARS HDL were very significantly higher than those of the control HDL (P<0.01). Conclusion: Oxidative modification of HDL can be induced by human arterial SMCs.
