CAJ | 학술논문

经热预处理（温度为25～45 ℃，部分实验为20～36 ℃，时间为5～10 min）的菠菜(Spinacia oleracea L.)叶绿体，严重影响其能量转换的各步反应。⑴循环光合磷酸化速率随处理温度增高而下降。⑵类囊体膜上腺三磷酶失活。⑶光照诱导叶绿体的质子吸收减小；叶绿体的9_氨基吖啶（9_AA）荧光猝灭减弱，但加二环己基碳二亚胺(DCCD)可以部分恢复9_AA荧光猝灭。⑷叶绿体的延迟发光和ΔA515 nm电色效应均出现明显变化。⑸免疫印迹反应结果表明，经45 ℃预处理叶绿体的膜上腺三磷酶出现解离，其α亚单位的蛋白量明显减少。⑹预处理温度超过33 ℃，叶绿体光系统Ⅰ介导的氧吸收速率也下降，在反应介质中加芥子碱可以部分恢复其氧吸收能力。就这些结果与膜透性变化、耦联因子复合物解离和自由基积累的关系进行了讨论。
경열예처리（온도위25～45 ℃，부분실험위20～36 ℃，시간위5～10 min）적파채(Spinacia oleracea L.)협록체，엄중영향기능량전환적각보반응。⑴순배광합린산화속솔수처리온도증고이하강。⑵류낭체막상선삼린매실활。⑶광조유도협록체적질자흡수감소；협록체적9_안기아정（9_AA）형광졸멸감약，단가이배기기탄이아알(DCCD)가이부분회복9_AA형광졸멸。⑷협록체적연지발광화ΔA515 nm전색효응균출현명현변화。⑸면역인적반응결과표명，경45 ℃예처리협록체적막상선삼린매출현해리，기α아단위적단백량명현감소。⑹예처리온도초과33 ℃，협록체광계통Ⅰ개도적양흡수속솔야하강，재반응개질중가개자감가이부분회복기양흡수능력。취저사결과여막투성변화、우련인자복합물해리화자유기적루적관계진행료토론。
Characterization of energy_transduction on the chloroplast thylakoid membranes from spinach (Spinacia oleracea L.) after thermal pretreatment was investigated. The related reactions of energy_transduction in chloroplasts were seriously affected by thermal pretreatment. The results were obtained as following: (1) The rate of cyclic photophosphorylation declined when the pretreatment temperature increased in the range of 25 to 45 ℃. (2) The thermal pretreatment led to a decrease of the activity of thylakoid membrane_bounded ATPase. (3) Proton uptake of chloroplasts and the fluorescence quenching of 9_aminoacridine (9_AA) in thylakoid membrane decreased after the thermal pretreatment, but addition of dicyclohexylcarbodiimide (DCCD) could partially restore the fluorescence quenching of 9_AA. (4) Both the rates of fast phase in electrochroism absorption change at 515 nm and the millisecond delayed light emission (ms_DLE) of chloroplast showed a progressive decrease upon raising the temperature of pretreatment. (5) Immunbloting analysis showed that the thermal pretreatment caused the changes of protein content and the electrophoresis mobility of thylakoid membrane_bound ATPase and its α_subunit. (6) If the temperature of pretreatment were higher than 33 ℃, oxygen uptake of PSⅠ _mediated in the samples was rapidly inhibited, but addition of sinapine into the reaction medium could partially restore the ability of oxygen uptake in the samples. These results are briefly discussed in relation to the change of permeability of thylakoid membranes, the dissociation of coupling factor complex as well as accumulation of the radicals in the thylakoid membranes after thermal pretreatment.