植物生理与分子生物学学报
植物生理與分子生物學學報
식물생리여분자생물학학보
JOURNAL OF PLANT PHYSIOLOGY AND MOLECULAR BIOLOGY
2004年
3期
291-296
,共6页
贺军民%佘小平%孟朝妮%赵文明
賀軍民%佘小平%孟朝妮%趙文明
하군민%사소평%맹조니%조문명
绿豆%UV-B辐射%Rubisco含量%H2O2含量%蛋白水解酶
綠豆%UV-B輻射%Rubisco含量%H2O2含量%蛋白水解酶
록두%UV-B복사%Rubisco함량%H2O2함량%단백수해매
Phaseolus raditus L.%UV-B radiation%Rubisco%H2O2%proteolytic activity
为了探讨UV-B辐射引起的Rubisco含量降低的可能机制,研究了两个绿豆品种(秦豆-20和中绿-1)幼苗在UV-B辐射下叶片Rubisco含量、蛋白水解酶活性和H2O2含量的变化.结果表明:UV-B辐射显著加速了两个绿豆品种幼苗叶片H2O2含量和蛋白水解酶活性上升,使Rubiscco含量下降.秦豆-20品种在UV-B辐射下H2O2含量和蛋白水解酶活性的上升程度明显大于中绿-1,相应其Rubisco含量的下降程度也大于中绿-1.抗坏血酸处理能明显降低UV-B辐射下两品种幼苗叶片H2O2含量,同时明显抑制蛋白水解酶活性的上升及Rubisco含量的下降.结果说明UV-B辐射诱导Rubisco含量的降低可能通过提高H2O2水平从而加强蛋白水解酶系统的活化而加速了Rubisco的降解.
為瞭探討UV-B輻射引起的Rubisco含量降低的可能機製,研究瞭兩箇綠豆品種(秦豆-20和中綠-1)幼苗在UV-B輻射下葉片Rubisco含量、蛋白水解酶活性和H2O2含量的變化.結果錶明:UV-B輻射顯著加速瞭兩箇綠豆品種幼苗葉片H2O2含量和蛋白水解酶活性上升,使Rubiscco含量下降.秦豆-20品種在UV-B輻射下H2O2含量和蛋白水解酶活性的上升程度明顯大于中綠-1,相應其Rubisco含量的下降程度也大于中綠-1.抗壞血痠處理能明顯降低UV-B輻射下兩品種幼苗葉片H2O2含量,同時明顯抑製蛋白水解酶活性的上升及Rubisco含量的下降.結果說明UV-B輻射誘導Rubisco含量的降低可能通過提高H2O2水平從而加彊蛋白水解酶繫統的活化而加速瞭Rubisco的降解.
위료탐토UV-B복사인기적Rubisco함량강저적가능궤제,연구료량개록두품충(진두-20화중록-1)유묘재UV-B복사하협편Rubisco함량、단백수해매활성화H2O2함량적변화.결과표명:UV-B복사현저가속료량개록두품충유묘협편H2O2함량화단백수해매활성상승,사Rubiscco함량하강.진두-20품충재UV-B복사하H2O2함량화단백수해매활성적상승정도명현대우중록-1,상응기Rubisco함량적하강정도야대우중록-1.항배혈산처리능명현강저UV-B복사하량품충유묘협편H2O2함량,동시명현억제단백수해매활성적상승급Rubisco함량적하강.결과설명UV-B복사유도Rubisco함량적강저가능통과제고H2O2수평종이가강단백수해매계통적활화이가속료Rubisco적강해.
To understand the role of H2O2 in the damaging effect of UV-B radiation on ribulose- 1,5-bisphosphate carboxylase/oxygenase (Rubisco)content, the time courses of changes in Rubisco and H2O2 contents under UV-B treatment were measured in the primary leaves of two mung bean cultivars (Phaseolus raditus L. cv. Zhonglu-1 and Qindou-20). A 0.4 W/m2 UV-B treatment caused a drop of the former to about 1/3 and a 30%-40%rise in H2O2 content in a week in both cultivars but with Qindou-20 showing stronger responses. Such higher rate of increase in proteolytic activity under UV-B treatment implies that UV-B lowers Rubisco by activating proteolytic enzymes which hydrolizes Rubisco. Scavenging of H2O2 by exogenously added ascorbic acid (ASA) significantly inhibited the UV-B-induced decrease in Rubisco amount and increases in H2O2 level and proteolytic activity. It is shown that H2O2 participates in the UV-B-induced reduction of Rubisco amount. It is suggested that H2O2 stimulates the degradation of Rubisco by activation of proteolytic systems.