植物检疫
植物檢疫
식물검역
PLANT QUARANTINE
2009年
4期
32-33
,共2页
丁元明%张巧萍%王云月%李旻%周剑%白永华%段禄华%和万忠
丁元明%張巧萍%王雲月%李旻%週劍%白永華%段祿華%和萬忠
정원명%장교평%왕운월%리민%주검%백영화%단록화%화만충
凤仙花坏死斑病毒%建兰花叶病毒%双重RT-PCR
鳳仙花壞死斑病毒%建蘭花葉病毒%雙重RT-PCR
봉선화배사반병독%건란화협병독%쌍중RT-PCR
Impatiens necrotic spot virus%Cymbidium mosaic virus%dual - RT - PCR
根据凤仙花坏死斑病毒(Impatiens necrotic spot vjrus,INSV)和建兰花叶病毒(Cymbidium mosaic virus,CyMV)的保守序列设计特异性引物,建立了双重RT-PCR同时检测两种病毒的方法.用该方法对兰花基地的可疑发病兰株进行检测,结果从田间采取的12株可疑兰花中有6株检出INSV,1株检出CyMV,2株同时检出两种病毒.
根據鳳仙花壞死斑病毒(Impatiens necrotic spot vjrus,INSV)和建蘭花葉病毒(Cymbidium mosaic virus,CyMV)的保守序列設計特異性引物,建立瞭雙重RT-PCR同時檢測兩種病毒的方法.用該方法對蘭花基地的可疑髮病蘭株進行檢測,結果從田間採取的12株可疑蘭花中有6株檢齣INSV,1株檢齣CyMV,2株同時檢齣兩種病毒.
근거봉선화배사반병독(Impatiens necrotic spot vjrus,INSV)화건란화협병독(Cymbidium mosaic virus,CyMV)적보수서렬설계특이성인물,건립료쌍중RT-PCR동시검측량충병독적방법.용해방법대란화기지적가의발병란주진행검측,결과종전간채취적12주가의란화중유6주검출INSV,1주검출CyMV,2주동시검출량충병독.
Designed a pairs of specific primers based on the conservative region of Impatiens necrotic spot virus and Cymbidium mosaic virus , a dual - RT - PCR technique was successfully adapted to detect the at the same time. This method used to detect'12 samples of orchid. The results showed that INSV could be detected in 7 sam-pies, and CyMV could be detected in 3 samples, while both INSV and CyMV could be detected in 1 samples at the same time.
