中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
CHINESE JOURNAL OF GERIATRIC CARDIOVASCULAR AND CEREBROVASCULAR DISEASES
2009年
8期
621-624
,共4页
武衡%周庆%黄开亮%汤显靖
武衡%週慶%黃開亮%湯顯靖
무형%주경%황개량%탕현정
脑出血%干细胞移植%脑源性神经营养因子%血管内皮生长因子A%免疫组织化学
腦齣血%榦細胞移植%腦源性神經營養因子%血管內皮生長因子A%免疫組織化學
뇌출혈%간세포이식%뇌원성신경영양인자%혈관내피생장인자A%면역조직화학
cerebral hemorrhage%stem cell transplantation%brain-derived neurotrophic factor%vas-cular endothelial growth factor A%immunohistochemistry
目的 观察大鼠脑出血模型脑内移植神经干细胞(NSCs)后脑源性神经营养因子(BDNF)和血管内皮生长因子(VEGF)的表达及对血管生成的影响.方法 SD大鼠140只,随机分为假手术组(A组)、脑出血模型组(B组)、NSCs培养液移植组(C组),NSCs移植组(D组),每组35只.免疫组织化学方法观察各组术后6 h,1、4、7、14、21和28天BDNF、VEGF及CD34标记的微血管密度的变化,并进行相关分析.结果 与A组比较,B组、C组BDNF的表达1天达高峰(P<0.05),D组表达高峰延迟至第4天,至28天仍高于A组(P<0.05);D组在所有时间点与B组比较,差异有统计学意义(P<0.05);D组在4天后的5个时间点与C组比较,差异有统计学意义(P<0.05).B组、C组VEGF和微血管密度表达模式相同,均在1天达高峰;D组呈双高峰表达,在第4天和14天,至28天仍高于B组、C组的表达水平(P<0.05),相关分析显示,VEGF与微血管密度呈正相关(r=0.911,P<0.05).结论 脑出血大鼠脑内移植NSCs后,脑内BDNF及VEGF维持长时间的高表达,微血管密度增加.
目的 觀察大鼠腦齣血模型腦內移植神經榦細胞(NSCs)後腦源性神經營養因子(BDNF)和血管內皮生長因子(VEGF)的錶達及對血管生成的影響.方法 SD大鼠140隻,隨機分為假手術組(A組)、腦齣血模型組(B組)、NSCs培養液移植組(C組),NSCs移植組(D組),每組35隻.免疫組織化學方法觀察各組術後6 h,1、4、7、14、21和28天BDNF、VEGF及CD34標記的微血管密度的變化,併進行相關分析.結果 與A組比較,B組、C組BDNF的錶達1天達高峰(P<0.05),D組錶達高峰延遲至第4天,至28天仍高于A組(P<0.05);D組在所有時間點與B組比較,差異有統計學意義(P<0.05);D組在4天後的5箇時間點與C組比較,差異有統計學意義(P<0.05).B組、C組VEGF和微血管密度錶達模式相同,均在1天達高峰;D組呈雙高峰錶達,在第4天和14天,至28天仍高于B組、C組的錶達水平(P<0.05),相關分析顯示,VEGF與微血管密度呈正相關(r=0.911,P<0.05).結論 腦齣血大鼠腦內移植NSCs後,腦內BDNF及VEGF維持長時間的高錶達,微血管密度增加.
목적 관찰대서뇌출혈모형뇌내이식신경간세포(NSCs)후뇌원성신경영양인자(BDNF)화혈관내피생장인자(VEGF)적표체급대혈관생성적영향.방법 SD대서140지,수궤분위가수술조(A조)、뇌출혈모형조(B조)、NSCs배양액이식조(C조),NSCs이식조(D조),매조35지.면역조직화학방법관찰각조술후6 h,1、4、7、14、21화28천BDNF、VEGF급CD34표기적미혈관밀도적변화,병진행상관분석.결과 여A조비교,B조、C조BDNF적표체1천체고봉(P<0.05),D조표체고봉연지지제4천,지28천잉고우A조(P<0.05);D조재소유시간점여B조비교,차이유통계학의의(P<0.05);D조재4천후적5개시간점여C조비교,차이유통계학의의(P<0.05).B조、C조VEGF화미혈관밀도표체모식상동,균재1천체고봉;D조정쌍고봉표체,재제4천화14천,지28천잉고우B조、C조적표체수평(P<0.05),상관분석현시,VEGF여미혈관밀도정정상관(r=0.911,P<0.05).결론 뇌출혈대서뇌내이식NSCs후,뇌내BDNF급VEGF유지장시간적고표체,미혈관밀도증가.
Objective To observe the expression of BDNF and VEGF and vasculogenesis in rats with intracerebral hemorrhage(ICH) after transplantation of NSCs. Method 140 SD rats were di-vided randomly into sham operated group(group A), model group(group B), NSCs culture medi-um transplanted group(group C) and NSCs transplanted group(group D). NSCs were transplan-ted into the subventricular zone in rat models of ICH,The expression changes of BDNF,VEGF,microvessel density(MVD) at 6 h and on days 1,4,7,14,21 and 28 after ICH was observed by means of immunohistochemical technique. Results Compared with group A, the expression of BDNF peaked on first day in groups B and C,and the expression peak was delayed to 4th day in group D,but maintained higher expression than group A on 28th day (P<0.05). At all time points,positive cell counts between group D and group B had significant difference (P<0.05).On days 4,7,14,21 and 28,positive cell counts between group D and group C had significant difference (P < 0.05). The expression levels of VEGF and MVD peaked on first day in groups B and C,and were higher in group C than in group B on days 1,4. Group D had double expression peaks on 4th day and 14th day,and maintained higher expression than in groups B,C on 28th day (P < 0.05). VEGF showed positive correlation with MVD (r=0.911 ,P<0.05). Conclusions The high expression of BDNF and VEGF is maintained for a long time and MVD increases in rats with cerebral hemorrhage after transplantation of NSCs.
