CAJ | 학술논문

[目的]寻找一种有效的磁铁矿尾矿宏基因组DNA的提取方法.[方法]分别采用直接提取法和SDS-高盐法提取邯邢矿区磁铁矿尾矿宏基因组DNA,比较2种方法的提取效果.[结果]直接提取法的提取效果较差,基本上没有提出DNA,SDS-高盐法的提取效果较好,DNA得率较高;23 S rDNA特异序列扩增结果显示,所提DNA能够满足PCR扩增的需要.[结论]所取尾矿样品中含有大量的金属离子,这些金属离子不但影响溶菌酶的活性,而且影响后续PCR扩增效率,所以提取DNA前需对样品进行高浓度TE处理.
[목적]심조일충유효적자철광미광굉기인조DNA적제취방법.[방법]분별채용직접제취법화SDS-고염법제취함형광구자철광미광굉기인조DNA,비교2충방법적제취효과.[결과]직접제취법적제취효과교차,기본상몰유제출DNA,SDS-고염법적제취효과교호,DNA득솔교고;23 S rDNA특이서렬확증결과현시,소제DNA능구만족PCR확증적수요.[결론]소취미광양품중함유대량적금속리자,저사금속리자불단영향용균매적활성,이차영향후속PCR확증효솔,소이제취DNA전수대양품진행고농도TE처리.
[Objective]The aim was to seek one effective extraction method of metagenomic DNA from tailings of magnetite. [Method]The metagenomic DNA from tailings of magnetite in Hanxing mining area was extracted by direct extraction method and SDS-high salt extraction method. The extraction effects of 2 kinds of method were compared. [Result]The extraction effect of direct extraction method was worse, there was almost no DNA being extracted. The extraction effect of SDS-high salt extraction method was better, and the yield rate of DNA was higher. The results of 23 S rDNA specific sequences amplification showed that the extracted DNA could meet the requirements of PCR amplification. [Conclusion]The tested tailing samples contained large amounts of metal ion, and the metal ions not only had effect on activity of lysozyme, but also had effect on further PCR amplification efficiency. So the tailing samples should be treated by TE with high concn.