CAJ | 학술논문

以平贝母为供试材料,研究了组培平贝母培养过程中培养体不同发育时期同工酶的变化.结果表明:在发育过程中各培养体的酯酶同工酶的酶带较丰富,共检测出11条酶带,且形成小鳞茎后酶谱的酶带数都明显多于愈伤组织和胚状体时期,且愈伤组织在Rf =0.708处产生了特异性酶带.过氧化氢酶在愈伤组织、胚状体和刚形成小鳞茎时都有表达,且酶谱仅有1条带,Rf =0.042,而在鳞茎膨大时没有该酶的表达.
이평패모위공시재료,연구료조배평패모배양과정중배양체불동발육시기동공매적변화.결과표명:재발육과정중각배양체적지매동공매적매대교봉부,공검측출11조매대,차형성소린경후매보적매대수도명현다우유상조직화배상체시기,차유상조직재Rf =0.708처산생료특이성매대.과양화경매재유상조직、배상체화강형성소린경시도유표체,차매보부유1조대,Rf =0.042,이재린경팽대시몰유해매적표체.
Taking Fritillaria ussuriensis Maxim.as experiment material,the isozyme changes in different stage of tissue culture during culturing of Fritillaria ussuriensis Maxim.were studied.The results showed that enzyme bands of esterase isozyme were abundant and examined 11 bars;Enzyme bands were obviously more than callus and embryoid after forming small bulblet and callus presented differential band at Rf =0.708.Catalase isozyme expressed and had only one bar during calluse,embryoid and small bulblet formation,Rf was 0.042,while the catalase isozyme was not expressed in bulblet expanding.