CAJ | 학술논문

背景:重组人促红细胞生成素是一种糖蛋白,近年的研究表明其对神经细胞的许多功能活动均具有调节作用.目的:观察不同浓度重组人促红细胞生成素对神经干细胞体外培养增殖的影响.方法:提取新生SD大鼠神经干细胞,用含不同浓度(5,50,500 U/mL)重组人促红细胞生成素和20 μg/L碱性成纤维细胞生长因子的无血清培养基进行培养,以不含重组人促红细胞生成素无血清培养基为对照组.细胞培养7 d后计算神经干细胞克隆形成率,培养10 d后计数NSE和GFAP免疫阳性细胞数.结果与结论:添加重组人促红细胞生成素组细胞增殖较快,最终神经球的数量多于对照组,以50 U/mL重组人促红细胞生成素组作用显著;50 U/mL重组人促红细胞生成素组的生长速度显著快于对照组.50 U/mL重组人促红细胞生成素组中NSE和GFAP免疫阳性细胞明显多于对照组(P<0.01).结果表明重组人促红细胞生成素对神经干细胞体外培养增殖有促进作用,尤以适中浓度(50 U/mL) 作用更加明显.
배경:중조인촉홍세포생성소시일충당단백,근년적연구표명기대신경세포적허다공능활동균구유조절작용.목적:관찰불동농도중조인촉홍세포생성소대신경간세포체외배양증식적영향.방법:제취신생SD대서신경간세포,용함불동농도(5,50,500 U/mL)중조인촉홍세포생성소화20 μg/L감성성섬유세포생장인자적무혈청배양기진행배양,이불함중조인촉홍세포생성소무혈청배양기위대조조.세포배양7 d후계산신경간세포극륭형성솔,배양10 d후계수NSE화GFAP면역양성세포수.결과여결론:첨가중조인촉홍세포생성소조세포증식교쾌,최종신경구적수량다우대조조,이50 U/mL중조인촉홍세포생성소조작용현저;50 U/mL중조인촉홍세포생성소조적생장속도현저쾌우대조조.50 U/mL중조인촉홍세포생성소조중NSE화GFAP면역양성세포명현다우대조조(P<0.01).결과표명중조인촉홍세포생성소대신경간세포체외배양증식유촉진작용,우이괄중농도(50 U/mL) 작용경가명현.
BACKGROUND: Recombinant human erythropoietin (rhEPO) is a glycoprotein. Recent studies have demonstrated that rhEPO regulates many functional activities of neural cells. OBJECTIVE: To investigate the effects of different concentrations of rhEPO on proliferation of neural stem cells (NSCs) cultured in vitro. METHODS: Newborn Sprague-Dawley rat NSCs were harvested and cultured with serum-free culture medium containing different concentrations (5, 50, 500 U/mL) of rhEPO and 20 μg/L basic fibroblast growth factors (5, 50, and 500 U/mL rhEPO groups) and serum-free culture medium only containing 20 μg/L basic fibroblast growth factors (control group). After 7 days of culture, the cloning efficiency of NSCs was calculated. After 10 days of culture, neuron specific enolase (NSE)-and glial fibrillary acidic protein (GFAP)-immunoreactive cells were quantified. RESULTS AND CONCLUSION: In the rhEPO groups, cells proliferated rapidly, and the number of NSC microspheres was greater, in particular in the 50 U/mL rhEPO group, compared with the control group. NSCs grew faster in the 50 U/mL rhEPO group than in the control group. The number of NSE-and GFAP-immunoreactive cells was greater in the 50 U/mL rhEPO group than in the control group (P<0.01). These findings suggest that rhEPO promotes the in vitro culture and proliferation of NSCs, in particular 50 U/mL rhEPO.