CAJ | 학술논문

目的评价荧光定量PCR检测腹水细菌16S rRNA基因在诊断非中性粒细胞性腹水自发性细菌性腹膜炎患者中的临床应用价值.方法用细菌16S rRNA基因荧光定量PCR法检测64例非中性粒细胞性腹水疑似自发性细菌性腹膜炎患者及6例慢性肝病伴非感染性腹水患者腹水中的细菌DNA,同时与腹水细菌培养结果进行对比.结果细菌16S rRNA荧光定量PCR最低可检测到10个拷贝的DNA复制,检测细菌的阳性率明显高于细菌培养的阳性率,分别为15.63%和3.13%,两组差异有统计学意义(x2=5.52,P＜0.05).6例非感染性腹水荧光定量PCR及细菌培养均阴性.结论腹水细菌16S rRNA荧光定量PCR检测细菌的特异性强、敏感性高,在诊断非中性粒细胞性腹水自发性细菌性腹膜炎患者中具有重要的临床应用价值.
목적 평개형광정량PCR검측복수세균16S rRNA기인재진단비중성립세포성복수자발성세균성복막염환자중적림상응용개치.방법 용세균16S rRNA기인형광정량PCR법검측64례비중성립세포성복수의사자발성세균성복막염환자급6례만성간병반비감염성복수환자복수중적세균DNA,동시여복수세균배양결과진행대비.결과 세균16S rRNA형광정량PCR최저가검측도10개고패적DNA복제,검측세균적양성솔명현고우세균배양적양성솔,분별위15.63%화3.13%,량조차이유통계학의의(x2=5.52,P＜0.05).6례비감염성복수형광정량PCR급세균배양균음성.결론 복수세균16S rRNA형광정량PCR검측세균적특이성강、민감성고,재진단비중성립세포성복수자발성세균성복막염환자중구유중요적림상응용개치.
Objective To evaluate clinical applications on the quantitative PCR detection of ascitic bacterial 16S rRNA genes in the diagnosis of spontaneous bacterial peritonitis with non-neutrophil ascites. Methods The bacterial 16S rRNA was detected by quantitative fluorescent PCR in 64 cases of suspected spontaneous bacterial peritonitis with nonneutrophil ascites, 6 cases of chronic liver disease accompanied with non-infected ascites at the same time period, as compared to the ascitic bacterial culture. Results Bacterial 16S rRNA quantitative PCR can detect DNA duplicate as low as 10 copies. The rate of detectable bacterial 16S rRNA by quantitative PCR( 15.63% ) was significantly higher than the rate of positive bacterial culture (3.13% ) ( x2 = 5.52, P ＜ 0.05 ); and 6 cases of non-infected ascites were negative analyzed by quantitative fluorescent PCR and bacterial cultures. Conclusions The ascitic bacterial 16S rRNA quantitative PCR detection is high specificity and sensitivity for the diagnosis of spontaneous bacterial peritonitis with non-neutrophils ascites, whtich is of important clinical significance.