国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2010年
5期
305-308
,共4页
谌翠容%潘红英%孙洪运%陈群伟%徐晶%叶荣夏%娄国强%卢德荣
諶翠容%潘紅英%孫洪運%陳群偉%徐晶%葉榮夏%婁國彊%盧德榮
심취용%반홍영%손홍운%진군위%서정%협영하%루국강%로덕영
腹膜炎%细菌%16S rRNA%荧光定量PCR
腹膜炎%細菌%16S rRNA%熒光定量PCR
복막염%세균%16S rRNA%형광정량PCR
Peritonitis%Bacteria%16S rRNA%Quantitative fluorescent PCR
目的 评价荧光定量PCR检测腹水细菌16S rRNA基因在诊断非中性粒细胞性腹水自发性细菌性腹膜炎患者中的临床应用价值.方法 用细菌16S rRNA基因荧光定量PCR法检测64例非中性粒细胞性腹水疑似自发性细菌性腹膜炎患者及6例慢性肝病伴非感染性腹水患者腹水中的细菌DNA,同时与腹水细菌培养结果进行对比.结果 细菌16S rRNA荧光定量PCR最低可检测到10个拷贝的DNA复制,检测细菌的阳性率明显高于细菌培养的阳性率,分别为15.63%和3.13%,两组差异有统计学意义(x2=5.52,P<0.05).6例非感染性腹水荧光定量PCR及细菌培养均阴性.结论 腹水细菌16S rRNA荧光定量PCR检测细菌的特异性强、敏感性高,在诊断非中性粒细胞性腹水自发性细菌性腹膜炎患者中具有重要的临床应用价值.
目的 評價熒光定量PCR檢測腹水細菌16S rRNA基因在診斷非中性粒細胞性腹水自髮性細菌性腹膜炎患者中的臨床應用價值.方法 用細菌16S rRNA基因熒光定量PCR法檢測64例非中性粒細胞性腹水疑似自髮性細菌性腹膜炎患者及6例慢性肝病伴非感染性腹水患者腹水中的細菌DNA,同時與腹水細菌培養結果進行對比.結果 細菌16S rRNA熒光定量PCR最低可檢測到10箇拷貝的DNA複製,檢測細菌的暘性率明顯高于細菌培養的暘性率,分彆為15.63%和3.13%,兩組差異有統計學意義(x2=5.52,P<0.05).6例非感染性腹水熒光定量PCR及細菌培養均陰性.結論 腹水細菌16S rRNA熒光定量PCR檢測細菌的特異性彊、敏感性高,在診斷非中性粒細胞性腹水自髮性細菌性腹膜炎患者中具有重要的臨床應用價值.
목적 평개형광정량PCR검측복수세균16S rRNA기인재진단비중성립세포성복수자발성세균성복막염환자중적림상응용개치.방법 용세균16S rRNA기인형광정량PCR법검측64례비중성립세포성복수의사자발성세균성복막염환자급6례만성간병반비감염성복수환자복수중적세균DNA,동시여복수세균배양결과진행대비.결과 세균16S rRNA형광정량PCR최저가검측도10개고패적DNA복제,검측세균적양성솔명현고우세균배양적양성솔,분별위15.63%화3.13%,량조차이유통계학의의(x2=5.52,P<0.05).6례비감염성복수형광정량PCR급세균배양균음성.결론 복수세균16S rRNA형광정량PCR검측세균적특이성강、민감성고,재진단비중성립세포성복수자발성세균성복막염환자중구유중요적림상응용개치.
Objective To evaluate clinical applications on the quantitative PCR detection of ascitic bacterial 16S rRNA genes in the diagnosis of spontaneous bacterial peritonitis with non-neutrophil ascites. Methods The bacterial 16S rRNA was detected by quantitative fluorescent PCR in 64 cases of suspected spontaneous bacterial peritonitis with nonneutrophil ascites, 6 cases of chronic liver disease accompanied with non-infected ascites at the same time period, as compared to the ascitic bacterial culture. Results Bacterial 16S rRNA quantitative PCR can detect DNA duplicate as low as 10 copies. The rate of detectable bacterial 16S rRNA by quantitative PCR( 15.63% ) was significantly higher than the rate of positive bacterial culture (3.13% ) ( x2 = 5.52, P < 0.05 ); and 6 cases of non-infected ascites were negative analyzed by quantitative fluorescent PCR and bacterial cultures. Conclusions The ascitic bacterial 16S rRNA quantitative PCR detection is high specificity and sensitivity for the diagnosis of spontaneous bacterial peritonitis with non-neutrophils ascites, whtich is of important clinical significance.