CAJ | 학술논문

目的研究两种端粒酶抑制剂干扰素α 2b(IFN-α 2b)和顺铂(CDDP)对人葡萄膜黑色素瘤细胞生长抑制作用,为临床化疗人葡萄膜黑色素瘤提供更多依据.方法用不同浓度及不同作用时间的IFN-α2b和CDDP作用于体外培养的人葡萄膜黑色素瘤细胞.倒置显微镜、电子显微镜观察其形态学变化,并用MTT法检测干扰剂对细胞生长的抑制作用.结果随着IFN-α 2b和CDDP浓度的增加及作用时间的延长,细胞出现生长抑制的形态学变化,电子显微镜显示细胞出现凋亡;作用72 h,IFN-α 2b和CDDP浓度分别大于500IU/ml及1.00mg/L时细胞出现抑制状态,并在浓度分别达到5000IU/ml及10.00 mg/L时其细胞抑制率(66.1±4.7)%及(74.0±3.3)%开始与对照组出现差异有统计学意义(P<0.001);IFN-α 2b(5000 IU/ml)和CDDP(10.00 mg/L)浓度同定,作用时间达到48 h后细胞出现抑制状态,72 h后抑制率(67.9±8.1)%及(76.5±1.6)%开始与对照组出现差异有统计学意义(P<0.001);即随着两者浓度升高及作用时间增长,葡萄膜黑色素瘤细胞受到的抑制作用逐渐增强(P<0.01).结论端粒酶抑制剂IFN-α2b和CDDP可有效抑制体外培养的人葡萄膜黑色素瘤细胞的生长,且随着两者浓度升高及作用时间延长抑制作用加强,可造成细胞凋亡.
목적 연구량충단립매억제제간우소α 2b(IFN-α 2b)화순박(CDDP)대인포도막흑색소류세포생장억제작용,위림상화료인포도막흑색소류제공경다의거.방법 용불동농도급불동작용시간적IFN-α2b화CDDP작용우체외배양적인포도막흑색소류세포.도치현미경、전자현미경관찰기형태학변화,병용MTT법검측간우제대세포생장적억제작용.결과 수착IFN-α 2b화CDDP농도적증가급작용시간적연장,세포출현생장억제적형태학변화,전자현미경현시세포출현조망;작용72 h,IFN-α 2b화CDDP농도분별대우500IU/ml급1.00mg/L시세포출현억제상태,병재농도분별체도5000IU/ml급10.00 mg/L시기세포억제솔(66.1±4.7)%급(74.0±3.3)%개시여대조조출현차이유통계학의의(P<0.001);IFN-α 2b(5000 IU/ml)화CDDP(10.00 mg/L)농도동정,작용시간체도48 h후세포출현억제상태,72 h후억제솔(67.9±8.1)%급(76.5±1.6)%개시여대조조출현차이유통계학의의(P<0.001);즉수착량자농도승고급작용시간증장,포도막흑색소류세포수도적억제작용축점증강(P<0.01).결론 단립매억제제IFN-α2b화CDDP가유효억제체외배양적인포도막흑색소류세포적생장,차수착량자농도승고급작용시간연장억제작용가강,가조성세포조망.
Objective To study the restrain effects of two telomerase inhibitors included IFN-α2b (interferon-α2b)and CDDP(cisplatin;cis-dichlorodiamine platinum)to uveal melanoma cells of human eyes,and to provide theory bases for chemotherary of uveal melanoma.Methods Applied IFN-α2b and CDDP to the primary cultured uveal melanoma cells of human eyes individually with different concentration and duration,and compared with control group.Morphological changes were observed by inverted optical and electronic microscope,and the restrain effects were detected by MTT.Results More and more morphological changes of restraint and apoptosis of cells were observed with the increasing concentration and duration of IFN-α 2b and CDDP.When action durations were 72 h.the cells were inhibited with the concentration more than 500 IU/ml(IFN-α 2b)and 1.00 mg/L(CDDP)respectively.The cells restrain rates were(66.1±4.7)%and(74.0+3.3)%with the concentration more than 5000 IU/ml(IFN-α2b)and 10 mg/L (CDDP),and the rates were higher than before(P<0.001).When concentrations were 5000 IU/ml(IFN-α2b)and 10.00 mg/L(CDDP),cells were inhibited at 48 h and the restrain rates were(67.9±8.1)%and(76.5±1.61%at 72 h,respectively,(P<O.001).With action duration or concentration of telomerase inhibitor increasing.the restrain effect on uveal melanoma cells of human eyes gradually increased(P<0.01).Conclusion As two effective telomerase inhibitors,IFN-α2b and CDDP,can effectively restrain growth of uveal melanoma cells effectively.The restrain effect gradually increased with action duration or concentration of telomerase inhibitor increasing,and it can cause cultured ceils apoptosis.