CAJ | 학술논문

目的探讨狼疮肾炎(LN)肾核因子(NF)-κB(NF-κB)的表达和外周血树突状细胞(DCs)表面标志的改变.方法对40例LN患者肾组织,用碱性磷酸酶-小鼠抗碱性磷酸酶免疫复合物法(APAAP)测定NF-κB表达.采用密度梯度离心法分离外周血DCs.结果 LN患者肾组织中,NF-κB在肾小球及肾小管均呈高水平的表达;他克莫司处理后的DCs较未处理组CD80+、CD86+、HLA-DR+表达下降;他克莫司处理前后对DCs的形态无明显影响;他克莫司处理后的DCs能明显抑制T细胞增殖及T细胞向Th2细胞转化.结论他克莫司在体外可抑制LN患者外周血DCs的成熟,NF-κB在LN肾组织局部的表达与LN的发生和发展有关.
목적 탐토랑창신염(LN)신핵인자(NF)-κB(NF-κB)적표체화외주혈수돌상세포(DCs)표면표지적개변.방법 대40례LN환자신조직,용감성린산매-소서항감성린산매면역복합물법(APAAP)측정NF-κB표체.채용밀도제도리심법분리외주혈DCs.결과 LN환자신조직중,NF-κB재신소구급신소관균정고수평적표체;타극막사처리후적DCs교미처리조CD80+、CD86+、HLA-DR+표체하강;타극막사처리전후대DCs적형태무명현영향;타극막사처리후적DCs능명현억제T세포증식급T세포향Th2세포전화.결론 타극막사재체외가억제LN환자외주혈DCs적성숙,NF-κB재LN신조직국부적표체여LN적발생화발전유관.
Objective To explore expression of nuclear factor kappa B (NF-κB) and change of peripheral blood dendritic cells(DCs) surface. Methods The expression of NF-κB and DCs in kidney of 40 LN cases were measured,by ALP-anti-ALP mice immune complex law(APAAP) and density gradient separation centrifugal meth-ods. Results kidney tissue of LN patients, NF-κB in the glomeruli and tubules showed a high level of expression ; CD80+ ,CD86+, HLA-DR+ expression decreasedtacrolimus group than the control group Division before and after the shape of the DCs had no effect on tacrolimus after the DCs could inhibit the proliferation of T cells and T cells to Th2 cell transformation. Conclusinn In vitro Tacrolimus inhibits the DCs mature in LN patients, NF-κB in LN kidney LN local expression and the occurrence and development of related.