CAJ | 학술논문

目的研究兔VX2移植瘤氩氦靶向冷冻治疗系统(简称氩氦刀)治疗后PET/CT显像及病理学的变化,观察治疗后肿瘤组织的演化过程,探索氩氦刀治疗后疗效评价的方法 .方法 36只日本大白兔在兔VX2肿瘤种植后第4周,进行~(18)F-脱氧葡萄糖(FDG)PET/CT显像;显像后进行氩氦刀治疗.术后按随机数字表将兔完全随机分为6组,每组6只.分别在氩氦刀治疗后第1,3,7,14,30和60天进行PET/CT显像;分析PET/CT图像,观察标准摄取值(SUV)的变化;第2次PET/CT显像(术后显像)后处死大白兔,取出原肿瘤组织,进行病理学检查和免疫组织化学检测,比较病理学和免疫组织化学的变化.统计软件为SPSS 16.0,组间比较采用配对资料的t检验和双变量相关分析,P＜0.05为差异有统计学意义.结果氩氦刀治疗后病理学检查提示,治疗区呈现坏死-炎性反应-机化过程.CT表现:肿瘤区域术后第3天至第14天有一短暂性增大,然后呈缩小的趋势;最大SUV(SUV_(max))术后第1天即出现明显下降(由治疗前2.54±1.12下降至0.67±0.12),第3天轻度升高(1.71±0.82),然后逐渐减低(治疗后第60天为0.51±0.32),治疗前后SUV_(max)变化差异有统计学意义(t=5.471,8.716,11.388,5.713,7.144和7.213,P均＜0.05).治疗前后靶区面积变化与SUV_(max)变化相关性不明显(r=0.259,P=0.675).治疗前后肿瘤微血管密度变化与SUV_(max)变化密切相关(r=0.865,P=0.032).结论 PET/CT显像可真实反映肿瘤组织氩氦刀治疗后的变化过程,是评价氩氦冷冻治疗疗效较理想的方法 .
목적 연구토VX2이식류아양파향냉동치료계통(간칭아양도)치료후PET/CT현상급병이학적변화,관찰치료후종류조직적연화과정,탐색아양도치료후료효평개적방법 .방법 36지일본대백토재토VX2종류충식후제4주,진행~(18)F-탈양포도당(FDG)PET/CT현상;현상후진행아양도치료.술후안수궤수자표장토완전수궤분위6조,매조6지.분별재아양도치료후제1,3,7,14,30화60천진행PET/CT현상;분석PET/CT도상,관찰표준섭취치(SUV)적변화;제2차PET/CT현상(술후현상)후처사대백토,취출원종류조직,진행병이학검사화면역조직화학검측,비교병이학화면역조직화학적변화.통계연건위SPSS 16.0,조간비교채용배대자료적t검험화쌍변량상관분석,P＜0.05위차이유통계학의의.결과 아양도치료후병이학검사제시,치료구정현배사-염성반응-궤화과정.CT표현:종류구역술후제3천지제14천유일단잠성증대,연후정축소적추세;최대SUV(SUV_(max))술후제1천즉출현명현하강(유치료전2.54±1.12하강지0.67±0.12),제3천경도승고(1.71±0.82),연후축점감저(치료후제60천위0.51±0.32),치료전후SUV_(max)변화차이유통계학의의(t=5.471,8.716,11.388,5.713,7.144화7.213,P균＜0.05).치료전후파구면적변화여SUV_(max)변화상관성불명현(r=0.259,P=0.675).치료전후종류미혈관밀도변화여SUV_(max)변화밀절상관(r=0.865,P=0.032).결론 PET/CT현상가진실반영종류조직아양도치료후적변화과정,시평개아양냉동치료료효교이상적방법 .
Objective To study the correlation of ~(18)F-fluorodeoxyglucose (FDG) PET/CT with pathological changes of the VX2 rabbit tumors after treatment of Ar-He knife,and to explore the evolution of the Ar-He knife curative effect for VX2 rabbit tumors.Methods Thirty-six Japanese white rabbits had successfully been implanted with VX2 tumors in thighs.Four weeks later,the rabbits with VX2 tumors were imaged with FDG PET/CT before they were treated with Ar-He cryoablation.The rabbits were evenly and randomly divided into 6 groups (6 rabbits in each group) and imaged with FDG PET/CT respectively on the first day,third day,seventh day,fourteenth day,thirtieth day and sixtieth day after cryoablation.The rabbits in each group were sacriftced after post-treatment FDG PET/CT imaging for pathology and immunohistochemistry studies.The standardized uptake value (SUV) of tumor regions were calculated and compared with pathology and immunohistochemistry findings in the cryoablative area in each group.Paired-samples t-test and bivariate correlation analysis were evaluated by statistical software SPSS 16.0.Results After ArHe cryoablation,pathological changes of "necrosis-inflammatory response→organization" were found.On CT imaging,the tumors enlarged during 3-14 d after treatment and then shrank gradually.On FDG PET imaging,the maximum SUV (SUV_(max)) dropped dramatically on the first day after the operation(from 2.54±1.12 to 0.67±0.12),and increased slightly on the third day (1.71±0.82),and then continually dropped to 0.51±0.32 (60 d afterthe operation).The differences of SUV_(max) between pre-and after cryoablationin each stage were significant,respectively (t=5.471,8.716,11.388,5.713,7.144 and 7.213,all P＜0.05).The size and SUV_(max) of the targeting area did not correlate with each other(r=0.259,P=0.675).The change of the MVD closely correlated with SUV_(max)(r=0.865,P=0.032).Conclusion FDG PET/CT can reveal the pathological change of tumor tissue after Ar-He cryoablation therapy and therefore may be a potential tool for evaluating the curative effect of this treatment modality.