CAJ | 학술논문

目的探讨瑞芬太尼对失血性休克兔急性肺损伤时脂质过氧化反应的影响.方法健康成年兔32只,随机分为4组(n=8):假手术组(S组)、急性肺损伤组(ALI组)、低剂量瑞芬太尼组(LR组)和高剂量瑞芬太尼组(HR组).ALI组、LR组和HR组经10 min股动脉放血至35～45 mm Hg制备急性肺损伤模型,LR组、HR组分别于放血前15 min静脉输注瑞芬太尼0.66、1.32μg·kg-1·min-1至处死动物.ALI组给予等容量生理盐水.于放血即刻(T1)、放血开始后20(T2)、70(T3)和100 min(T4)时经左侧股动脉采集血样,行血气分析,随后处死,取肺组织,光镜下观察肺组织病理学结果,计算肺湿干重比(W/D),测定MDA含量和SOD活性.结果与S组比较,ALI组W/D、MDA含量升高,SOD活性降低(P＜0.05);与ALI组比较,LR组T2时pH值、T2～4时PaO2升高,HR组T2-4时pH值和PaCO2升高,LR组和HR组W/D、MDA含量降低,SOD活性升高(P＜0.05);与LR组比较,HR组W/D、MDA含量降低,SOD活性升高(P＜0.05).LR组和HR组肺组织病理学损伤程度较ALI组减轻,HR组较LR组减轻.结论瑞芬太尼可通过抑制脂质过氧化反应减轻失血性休克兔急性肺损伤.
목적 탐토서분태니대실혈성휴극토급성폐손상시지질과양화반응적영향.방법 건강성년토32지,수궤분위4조(n=8):가수술조(S조)、급성폐손상조(ALI조)、저제량서분태니조(LR조)화고제량서분태니조(HR조).ALI조、LR조화HR조경10 min고동맥방혈지35～45 mm Hg제비급성폐손상모형,LR조、HR조분별우방혈전15 min정맥수주서분태니0.66、1.32μg·kg-1·min-1지처사동물.ALI조급여등용량생리염수.우방혈즉각(T1)、방혈개시후20(T2)、70(T3)화100 min(T4)시경좌측고동맥채집혈양,행혈기분석,수후처사,취폐조직,광경하관찰폐조직병이학결과,계산폐습간중비(W/D),측정MDA함량화SOD활성.결과 여S조비교,ALI조W/D、MDA함량승고,SOD활성강저(P＜0.05);여ALI조비교,LR조T2시pH치、T2～4시PaO2승고,HR조T2-4시pH치화PaCO2승고,LR조화HR조W/D、MDA함량강저,SOD활성승고(P＜0.05);여LR조비교,HR조W/D、MDA함량강저,SOD활성승고(P＜0.05).LR조화HR조폐조직병이학손상정도교ALI조감경,HR조교LR조감경.결론 서분태니가통과억제지질과양화반응감경실혈성휴극토급성폐손상.
Objective To investigate the effects of remifentanil on lipid peroxidation druing hemorrhagic shock-induced acute lung injury (ALI) in rabbits. Methods Thirty-two healthy adult rabbits weighing 2.0-2.5 kg were randomly divided into 4 groups (n = 8 each): sham operation group (group S); ALI group; low-dose remifentanil group (group LR); high-dose remifentanil group (group HR). The left femoral artery was cannulated for blood-letting and blood sampling. The right femoral artery was cannulated for remifentanil administration. The model of hemorrhagic shock was established by modified Wigger' s methods. In group S, only cannulation was performed. In group LR and HR, remifentanil was infused intraperitoneally at 0.66 and 1.32 μg·kg-1 ·min-1 15 min before blood-letting respectively, while group ALI received equal volume of normal saline instead. Arterial blood samples were taken at 0, 20,70 and 100 min after blood-letting (T1-4) for blood gas analysis. The animals were then sacrificed and the lungs were immediately removed for histological examination with light microscope and determination of W/D lung weight ratio, MDA content and SOD activity. Results W/D ratio and MDA content were significantly increased, while SOD activity was significantly decreased in group ALI compared with group S (P ＜0.05). The pH value at T2 and PaO2 at T2-4 in group LR and the pH value and PaCO2 at T2-4 in group HR were significantly higher than those in group ALI (P ＜ 0.05). W/D ratio and MDA content were significantly lower,while SOD activity was significantly higher in group LR and HR than in group ALI, and in group HR than in group LR (P ＜ 0.05). Remifentanil infusion significantly attenuated the pathologic changes in a dose-dependent manner. Conclusion Remifentanil pretreatment can attenuate hemorrhagic shock-induced ALI through inhibiting lipid peroxidation in rabbits.