中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2008年
9期
838-842
,共5页
RECK%基质金属蛋白酶-9%外周血单个核细胞%系统性红斑狼疮
RECK%基質金屬蛋白酶-9%外週血單箇覈細胞%繫統性紅斑狼瘡
RECK%기질금속단백매-9%외주혈단개핵세포%계통성홍반랑창
Reversion-inducing cysteine-rich protein with kazal motifs%Matrix metalloproteinase-9%Peripheral blood mononuclear cell%Systemic lupus erythematosus
目的 探讨系统性红斑狼疮(SEE)患者PBMC RECK(reversion-inducing cysteine-richprotein with kazal motif)的表达及其与基质金属蛋白酶-9(MMP-9)的关系.方法 分别用Western blot及RT-PCR检测SLE患者及健康对照组PBMC上RECK的蛋白及mRNA水平,同时检测MMP-9mRNA的水平.加入植物血凝素(PHA)刺激后检测3者的表达变化及刺激后MMP-9的分泌情况并与空白对照组比较.结果 与健康对照组相比,患者组RECK蛋白及mRNA水平降低,MMP-9 mRNA水平增高,分泌MMP-9的能力高于健康对照组.与空白对照相比,PHA刺激后,患者组及对照组RECK蛋白及mRNA表达均降低,MMP-9 mRNA水平升高,MMP-9分泌均增多.RECK的表达与MMP-9的分泌呈负相关.结论 RECK可能通过抑制MMP-9的分泌在SEE发病过程中起重要作用,对其的调控可望为SLE治疗提供新的方向.
目的 探討繫統性紅斑狼瘡(SEE)患者PBMC RECK(reversion-inducing cysteine-richprotein with kazal motif)的錶達及其與基質金屬蛋白酶-9(MMP-9)的關繫.方法 分彆用Western blot及RT-PCR檢測SLE患者及健康對照組PBMC上RECK的蛋白及mRNA水平,同時檢測MMP-9mRNA的水平.加入植物血凝素(PHA)刺激後檢測3者的錶達變化及刺激後MMP-9的分泌情況併與空白對照組比較.結果 與健康對照組相比,患者組RECK蛋白及mRNA水平降低,MMP-9 mRNA水平增高,分泌MMP-9的能力高于健康對照組.與空白對照相比,PHA刺激後,患者組及對照組RECK蛋白及mRNA錶達均降低,MMP-9 mRNA水平升高,MMP-9分泌均增多.RECK的錶達與MMP-9的分泌呈負相關.結論 RECK可能通過抑製MMP-9的分泌在SEE髮病過程中起重要作用,對其的調控可望為SLE治療提供新的方嚮.
목적 탐토계통성홍반랑창(SEE)환자PBMC RECK(reversion-inducing cysteine-richprotein with kazal motif)적표체급기여기질금속단백매-9(MMP-9)적관계.방법 분별용Western blot급RT-PCR검측SLE환자급건강대조조PBMC상RECK적단백급mRNA수평,동시검측MMP-9mRNA적수평.가입식물혈응소(PHA)자격후검측3자적표체변화급자격후MMP-9적분비정황병여공백대조조비교.결과 여건강대조조상비,환자조RECK단백급mRNA수평강저,MMP-9 mRNA수평증고,분비MMP-9적능력고우건강대조조.여공백대조상비,PHA자격후,환자조급대조조RECK단백급mRNA표체균강저,MMP-9 mRNA수평승고,MMP-9분비균증다.RECK적표체여MMP-9적분비정부상관.결론 RECK가능통과억제MMP-9적분비재SEE발병과정중기중요작용,대기적조공가망위SLE치료제공신적방향.
Objective To investigate the expression of RECK(reversion-inducing eysteine-rich protein with kazal motif) in peripheral blood mononuclear cell (PBMC) of systemic lupus erytbematosus (SLE) patients and the relationship between RECK and matrix metalloproteinase-9 (MMP-9) in SLE. Methods PBMC were isolated from 69 SLE patients and 23 healthy donors. The protein and mRNA levels of RECK in PBMC were determined by Western blot and RT-PCR, respectively, and the MMP-9 mRNA level was tested too. After cultured for 24 h with phytohemagglutinin(PHA) stimulation, these levels and the secretion of MMP-9 were observed. Results Compared with healthy donors, RECK protein and mRNA lev-els decreased in SLE patients, and MMP-9 mRNA level increased, and the secretion level of MMP-9 was higher. After PHA stimulation, compared with blank control, the expression of RECK protein and mRNA were lower, but the MMP-9 mRNA level increased and the secretion of MMP-9 increased significantly. The protein levels of RECK were inversely associated with MMP-9 secretion but no association between RECK protein level and MMP-9 mRNA level was found. Conclusion RECK might play an important role in SLE pathogenesis through inhibiting the secretion of MMP-9. Regulation of RECK expression would be prospec-tive in SLE treatment.
