CAJ | 학술논문

目的在腹泻住院患儿粪便标本中检测人双埃可病毒(HPeV),探讨HPeV与婴幼儿重症腹泻之间的联系.方法通过Real-time PCR直接从<5岁未知病原住院腹泻患儿粪便标本中检测HPeV,进而通过基于VP3/VP1连接区的巢式PCR扩增片段的测序结果进行分型.结果 99份腹泻粪便标本,HPeV阳性24例(24%),其中,以HPeV1为主(50%),其次为HPeV3(25%),HPeV4(8.3%),HPeV6(4.2%),还有3例HPeV阳性标本未能分型,未检测到HPeV2、5、7、8.HPeV感染的住院腹泻患儿主要为<1岁患儿.HPeV感染高峰为秋冬季节,10月达到峰值.VP3/VP1连接区基因与已知HPeV的病因构成及深入了解HPeV的病原学及流行病学特点有重要意义.
목적 재복사주원환인분편표본중검측인쌍애가병독(HPeV),탐토HPeV여영유인중증복사지간적련계.방법 통과Real-time PCR직접종<5세미지병원주원복사환인분편표본중검측HPeV,진이통과기우VP3/VP1련접구적소식PCR확증편단적측서결과진행분형.결과 99빈복사분편표본,HPeV양성24례(24%),기중,이HPeV1위주(50%),기차위HPeV3(25%),HPeV4(8.3%),HPeV6(4.2%),환유3례HPeV양성표본미능분형,미검측도HPeV2、5、7、8.HPeV감염적주원복사환인주요위<1세환인.HPeV감염고봉위추동계절,10월체도봉치.VP3/VP1련접구기인여이지HPeV적병인구성급심입료해HPeV적병원학급류행병학특점유중요의의.
Objective To detect human parechovirus (HPeV) from stool samples of hospitalized children for acute gastroonteritis of undetectable etiology. Methods We conducted a real-time PCR to detect HPeV. Result The results showed that 24 of 99 (24%) children with gastroenteritis of undetectable etiology were detected with HPeV. Four known HPeV types (HPeV1,3,4,6) were detected in the present study. HPeV1 (50%) was frequently identified as the predominant strain and follow by HPeV3 (25 % ), HPeV 4 (8.3 % ) and HPeV 6 (4.2 % ). We were unable to type 3 samples. Conclusion HPeV was prevalent in hospitalized children for acute gastreenteritis of undetectable etiology in China. Further study is needed for clarifying the role of HPeV in gastroenteritis.