CAJ | 학술논문

目的寻找与结直肠癌相关的蛋白质并建立结直肠癌血清蛋白指纹图谱诊断预测模型.方法随机选取结直肠癌病例36例(结直肠癌组)和疝或行胆囊择期手术的患者36例(对照组),术前静脉采血,采用弱阳离子交换蛋白质芯片(CM10),经表面加强激光解吸电离-飞行时间-质谱(SELDI-TOF-MS)技术测定,建立结直肠癌的血清蛋白指纹图谱诊断预测模型;选取88例结直肠癌患者和44例正常对照进行盲法验证.结果通过两组比较,得到5个差异蛋白峰,并以此建立诊断预测模型,其敏感性为100%,特异性为97.2%.盲法验证显示敏感性为71.6%,特异性为72.7%.其中质荷比为8908与13707的蛋白均存在于结直肠癌组和正常组的比较中,提示上述蛋白质与结直肠癌相关.结论运用SELDI-TOF-MS技术建立的血清蛋白指纹图谱模型对诊断结直肠癌具有较高的敏感性与特异性.质荷比为8908与13707的蛋白可能成为结直肠癌的肿瘤标记物.
목적 심조여결직장암상관적단백질병건립결직장암혈청단백지문도보진단예측모형.방법 수궤선취결직장암병례36례(결직장암조)화산혹행담낭택기수술적환자36례(대조조),술전정맥채혈,채용약양리자교환단백질심편(CM10),경표면가강격광해흡전리-비행시간-질보(SELDI-TOF-MS)기술측정,건립결직장암적혈청단백지문도보진단예측모형;선취88례결직장암환자화44례정상대조진행맹법험증.결과 통과량조비교,득도5개차이단백봉,병이차건립진단예측모형,기민감성위100%,특이성위97.2%.맹법험증현시민감성위71.6%,특이성위72.7%.기중질하비위8908여13707적단백균존재우결직장암조화정상조적비교중,제시상술단백질여결직장암상관.결론 운용SELDI-TOF-MS기술건립적혈청단백지문도보모형대진단결직장암구유교고적민감성여특이성.질하비위8908여13707적단백가능성위결직장암적종류표기물.
Objective To establish serum proteome fingerprinting predictive models and search for proteins associated with colorectal cancer.Methods Thirty-six randomly selected colorectal cancer patients and 36 cases with hernia or gall bladder diseases scheduled for elective operation were enrolled as cancer group and control group respectively.Peripheral venous blood samples were collected before the operations.Special serum protein or peptide fingerprint was investigated by using surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)measurement after blood sample had been treated with weak cation exchange protein chip(CM10)for each case.The obtained data were analyzed by Biomarker Wizard software to screen serum proteome tumor markers and set up diagnosis predictive model for eolorectal cancer.Blind validation of the model with 44 healthy controls and 88 colorectal cancer patients were carried out by using Biomarker Patterns Software.Results In comparing colorectal cancer group with control group,5 specific protein peaks(P<0.05)were found.The predictive model had a sensitivity of 100% and a specificity of 97.2%.A sensitivity of 71.6% and a specificity of 72.7% was got with the blind validation.The specific protein peaks with a mass-to-charge ratio(m/z)of 8908 and 13707 showed in all the results and it showed their strong relationship with colorectal cancer.Conclusions The predictive models built by the differences of serum proteome fingerprint could be a very useful diagnostic tool in colorectal cancer.Proteins with m/z of 8908 and 13707 would possibly be the tumor markers of colorectal cancer.