中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
47期
3349-3351
,共3页
夏强%刘卫彬%冯慧宇%陈振光%张莹%何雪桃
夏彊%劉衛彬%馮慧宇%陳振光%張瑩%何雪桃
하강%류위빈%풍혜우%진진광%장형%하설도
重症肌无力%胸腺%树突状细胞
重癥肌無力%胸腺%樹突狀細胞
중증기무력%흉선%수돌상세포
Myasthenia gravis%Thymus%Dendritic cell
目的 探讨胸腺成熟树突状细胞(mDC)数量与分布的改变与重症肌无力(MG)发生的关系.方法 选取胸腺切除前未行免疫抑制治疗的MG患者39例为研究组,同期因心脏病手术需行胸腺部分切除的患者19例为对照组.性别和年龄在两组中的分布差异无统计学意义(P>0.05).以DC-LAMP作为胸腺mDC的标志物,用免疫酶组织化学方法显示mDC在胸腺的表达情况,图像分析软件辅助分析.结果 (1)对照组mDC严格地限定在胸腺體质区和皮髓交界区;MG组mDC分布不规则,在髓质、皮质和间质均能见到.(2)对照组mDC大致呈均匀分布在髓质区.MG组则较多地见到成簇分布,尤其在生发中心周围往往出现mDC密集包绕表现.(3)mDC阳性区平均视野细胞相对比例和绝对数MG组(10.9%±2.2%,50±9)均高于对照组(8.5%±1.5%,41±7)(P<0.05).结论 MG患者胸腺组织存在mDC数量的增多及分布的异常.这种异常提示mDC可能积极地参与了MG的发病过程.
目的 探討胸腺成熟樹突狀細胞(mDC)數量與分佈的改變與重癥肌無力(MG)髮生的關繫.方法 選取胸腺切除前未行免疫抑製治療的MG患者39例為研究組,同期因心髒病手術需行胸腺部分切除的患者19例為對照組.性彆和年齡在兩組中的分佈差異無統計學意義(P>0.05).以DC-LAMP作為胸腺mDC的標誌物,用免疫酶組織化學方法顯示mDC在胸腺的錶達情況,圖像分析軟件輔助分析.結果 (1)對照組mDC嚴格地限定在胸腺體質區和皮髓交界區;MG組mDC分佈不規則,在髓質、皮質和間質均能見到.(2)對照組mDC大緻呈均勻分佈在髓質區.MG組則較多地見到成簇分佈,尤其在生髮中心週圍往往齣現mDC密集包繞錶現.(3)mDC暘性區平均視野細胞相對比例和絕對數MG組(10.9%±2.2%,50±9)均高于對照組(8.5%±1.5%,41±7)(P<0.05).結論 MG患者胸腺組織存在mDC數量的增多及分佈的異常.這種異常提示mDC可能積極地參與瞭MG的髮病過程.
목적 탐토흉선성숙수돌상세포(mDC)수량여분포적개변여중증기무력(MG)발생적관계.방법 선취흉선절제전미행면역억제치료적MG환자39례위연구조,동기인심장병수술수행흉선부분절제적환자19례위대조조.성별화년령재량조중적분포차이무통계학의의(P>0.05).이DC-LAMP작위흉선mDC적표지물,용면역매조직화학방법현시mDC재흉선적표체정황,도상분석연건보조분석.결과 (1)대조조mDC엄격지한정재흉선체질구화피수교계구;MG조mDC분포불규칙,재수질、피질화간질균능견도.(2)대조조mDC대치정균균분포재수질구.MG조칙교다지견도성족분포,우기재생발중심주위왕왕출현mDC밀집포요표현.(3)mDC양성구평균시야세포상대비례화절대수MG조(10.9%±2.2%,50±9)균고우대조조(8.5%±1.5%,41±7)(P<0.05).결론 MG환자흉선조직존재mDC수량적증다급분포적이상.저충이상제시mDC가능적겁지삼여료MG적발병과정.
Objective To investigate the correlation between onset of myasthenia gravis (MG) and the changes of the number and distribution of thymus mature dendritic cells (mDC).Methods The specimens of thymus were obtained during operation from 39 MG patients who hadn' t received immunosuppressive therapy before thymectomy and 19 sex- and nge-matched patients who underwent cardiosurgery as controls.Immunohistochemistry with antibody against DC-LAMP, specific surface marker of DC, was used to examine the number and distribution of thymus mDCs.Results(1) mDCs were restricted in the medulla and cortex-medulla junctional zone of thymus in the control group; while were irregularly distributed in the cortex, medulla, and stroma in the MG group.(2) mDCs presented a roughly uniform distribution in the medulla of thymus in the control group, while clustering distribution was more often in the MG group, especially dense around the germinal center.(3) The relative ratio and numbers of mDCs per average field of vision in the positive areas of the MG group were(10.9% ± 2.2%) and(50 ± 9), beth significantly higher than those of the control group [ (8.5% ± 1.5%) and (41 ±7) respectively, both P < 0.05 ].Conclusion The number and ratio of the thymus mDCs in MG are high, high, and the distribution abnormal.mDC actively may be actively involved in the pathogenesis of MG.
