中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2009年
5期
417-423
,共7页
罗敏%王志良%施沃栋%范先群%徐国彤
囉敏%王誌良%施沃棟%範先群%徐國彤
라민%왕지량%시옥동%범선군%서국동
糖尿病,实验性%糖尿病视网膜病变%透明质酸葡糖胺酶%纤溶酶%玻璃体脱离
糖尿病,實驗性%糖尿病視網膜病變%透明質痠葡糖胺酶%纖溶酶%玻璃體脫離
당뇨병,실험성%당뇨병시망막병변%투명질산포당알매%섬용매%파리체탈리
Diabetes mellitus,experimental%Diabetic retinopathy%Hyaluronoglucosaminidase%Plasmin%Vitreous detachment
目的 研究在糖尿病大鼠中能否采用药物性玻璃体松解术诱导完全性玻璃体后脱离(PVD).方法 实验研究.成年SD大鼠用链脲佐菌素(STZ)腹腔注射诱发糖尿病,4周后用视觉电生理、视网膜血管铺片、透射电镜观明确视网膜病变的发生.发病4周后将糖尿病大鼠分为4组:A组10只,右眼玻璃体内注射透明质酸酶5 U;B组10只,右眼玻璃体腔内注射纤溶酶0.5 U;C组10只,右眼玻璃体腔内注射纤溶酶0.5 U+透明质酸酶5 U;D组10只,右眼玻璃体注射BSS 2 μl.注射后一周内观察眼部一般情况并检查视觉电生理变化(F-ERG).一周时取各组大鼠视网膜做扫描电镜检查和组织切片,观察视网膜组织结构及玻璃体后脱离的发生情况.对数据采用t检验进行统计学分析.结果 糖尿病大鼠发病4周时已经有视觉电生理的改变,血管铺片显示周细胞的减少(t=6.1,P<0.01);Ops振幅降低、峰时延迟(t=2.8,3.4;P<0.05);透射电镜显示毛细血管周细胞水肿变性.玻璃体腔注射药物一周后,通过扫描电镜观察A组和D组无PVD发生(0/10);B组发生部分性PVD 6/10,完全性PVD 4/10;C组发生完全性PVD 10/10.视觉电生理检查各组与注射前相比差异均无统计学意义(P>0.05),组织切片检查未显示视网膜组织结构的异常改变.结论 STZ诱导糖尿病4周大鼠视网膜已经出现了病理改变,在其玻璃体腔中注射0.5 U的纤溶酶加5 U的透明质酸酶能够稳定地诱发出完全性PVD,而单独使用纤溶酶仅能诱发出部分性PVD,透明质酸酶不能诱发出PVD.各组未见药物对视网膜的结构和功能造成明显的毒性反应.
目的 研究在糖尿病大鼠中能否採用藥物性玻璃體鬆解術誘導完全性玻璃體後脫離(PVD).方法 實驗研究.成年SD大鼠用鏈脲佐菌素(STZ)腹腔註射誘髮糖尿病,4週後用視覺電生理、視網膜血管鋪片、透射電鏡觀明確視網膜病變的髮生.髮病4週後將糖尿病大鼠分為4組:A組10隻,右眼玻璃體內註射透明質痠酶5 U;B組10隻,右眼玻璃體腔內註射纖溶酶0.5 U;C組10隻,右眼玻璃體腔內註射纖溶酶0.5 U+透明質痠酶5 U;D組10隻,右眼玻璃體註射BSS 2 μl.註射後一週內觀察眼部一般情況併檢查視覺電生理變化(F-ERG).一週時取各組大鼠視網膜做掃描電鏡檢查和組織切片,觀察視網膜組織結構及玻璃體後脫離的髮生情況.對數據採用t檢驗進行統計學分析.結果 糖尿病大鼠髮病4週時已經有視覺電生理的改變,血管鋪片顯示週細胞的減少(t=6.1,P<0.01);Ops振幅降低、峰時延遲(t=2.8,3.4;P<0.05);透射電鏡顯示毛細血管週細胞水腫變性.玻璃體腔註射藥物一週後,通過掃描電鏡觀察A組和D組無PVD髮生(0/10);B組髮生部分性PVD 6/10,完全性PVD 4/10;C組髮生完全性PVD 10/10.視覺電生理檢查各組與註射前相比差異均無統計學意義(P>0.05),組織切片檢查未顯示視網膜組織結構的異常改變.結論 STZ誘導糖尿病4週大鼠視網膜已經齣現瞭病理改變,在其玻璃體腔中註射0.5 U的纖溶酶加5 U的透明質痠酶能夠穩定地誘髮齣完全性PVD,而單獨使用纖溶酶僅能誘髮齣部分性PVD,透明質痠酶不能誘髮齣PVD.各組未見藥物對視網膜的結構和功能造成明顯的毒性反應.
목적 연구재당뇨병대서중능부채용약물성파리체송해술유도완전성파리체후탈리(PVD).방법 실험연구.성년SD대서용련뇨좌균소(STZ)복강주사유발당뇨병,4주후용시각전생리、시망막혈관포편、투사전경관명학시망막병변적발생.발병4주후장당뇨병대서분위4조:A조10지,우안파리체내주사투명질산매5 U;B조10지,우안파리체강내주사섬용매0.5 U;C조10지,우안파리체강내주사섬용매0.5 U+투명질산매5 U;D조10지,우안파리체주사BSS 2 μl.주사후일주내관찰안부일반정황병검사시각전생리변화(F-ERG).일주시취각조대서시망막주소묘전경검사화조직절편,관찰시망막조직결구급파리체후탈리적발생정황.대수거채용t검험진행통계학분석.결과 당뇨병대서발병4주시이경유시각전생리적개변,혈관포편현시주세포적감소(t=6.1,P<0.01);Ops진폭강저、봉시연지(t=2.8,3.4;P<0.05);투사전경현시모세혈관주세포수종변성.파리체강주사약물일주후,통과소묘전경관찰A조화D조무PVD발생(0/10);B조발생부분성PVD 6/10,완전성PVD 4/10;C조발생완전성PVD 10/10.시각전생리검사각조여주사전상비차이균무통계학의의(P>0.05),조직절편검사미현시시망막조직결구적이상개변.결론 STZ유도당뇨병4주대서시망막이경출현료병리개변,재기파리체강중주사0.5 U적섬용매가5 U적투명질산매능구은정지유발출완전성PVD,이단독사용섬용매부능유발출부분성PVD,투명질산매불능유발출PVD.각조미견약물대시망막적결구화공능조성명현적독성반응.
Objective To evaluate the efficacy and safety of plasmin or hyaluronidase in the inducing of posterior vitreous detachment in diabetic rats. Methods Forty SD rats were induced diabetes by Streptozotocin (STZ). Four weeks later, these rats were randomized into 4 groups : rats in group A received 5 U hyaluronidase intravitral injection in right eyes;rats in group B received 0. 5 U plsmin intravitral injection in right eyes;rats in group C received 0. 5 U plasmin + 5 U hyaluroniclase intravitral injection in right eyes;rats in group D received BSS (balance salt solution ) 2 μl intravitral injection in right eyes. Clinical examination were performed at 1, 3, 7 days after injection. After 1 week, scanning electron microscope (SEM) was performed to judge whether the PVD was induced. ERG and histology were examined to evaluate the toxicity after the intravitreal injection of these two drugs. Results No PVD was found in SEM disclosed group A and group D. Forty percent eyes were induced complete PVD in groups B, and one hundred percent eyes were induced complete PVD in group C. ERG and histology showed no toxicity changes in any group. Conclusion Intravitreal injection of 0. 5 U plasmin + 5 U hayluronidase can induce complete PVD without obvious toxicity in diabetic rats. Solo usage of plasmin or hayluronidase can not induce complete PVD.
