CAJ | 학술논문

以霍山石斛苗总多糖(ST)和40%、50%、60%、80%终浓度的乙醇连续沉淀4种多糖组分(S1、S2、S3和S4)为材料,在超氧自由基、H2O2、羟基自由基、还原力、Fe2+-VC诱导的脂质过氧化和H2O2诱导的红细胞溶血等不同体系下进行霍山石斛多糖体外抗氧化研究.结果表明:不同相对分子质量霍山石斛多糖均有抗氧化作用,其中相对分子量最大的S1对H2O2、·OH的清除作用强于其他多糖组分,IC50分别为0.15mg/ml和1.81mg/ml,并具有显著的还原力和抑制Fe2+-VC诱导脂质过氧化能力,最大效应分别为0.312和45.67%,表明霍山石斛多糖的抗氧化能力与其相对分子质量大小有关.
이곽산석곡묘총다당(ST)화40%、50%、60%、80%종농도적을순련속침정4충다당조분(S1、S2、S3화S4)위재료,재초양자유기、H2O2、간기자유기、환원력、Fe2+-VC유도적지질과양화화H2O2유도적홍세포용혈등불동체계하진행곽산석곡다당체외항양화연구.결과표명:불동상대분자질량곽산석곡다당균유항양화작용,기중상대분자량최대적S1대H2O2、·OH적청제작용강우기타다당조분,IC50분별위0.15mg/ml화1.81mg/ml,병구유현저적환원력화억제Fe2+-VC유도지질과양화능력,최대효응분별위0.312화45.67%,표명곽산석곡다당적항양화능력여기상대분자질량대소유관.
Total polysaccharides extracted from seedlings of Dendrobium huoshanense C.Z. Tang et S.J. Cheng were subjected to a sequential precipitation with ethanol at 40%, 50%, 60% and 80% to obtain four fractions of S1, S2,S3 and S4. The antioxidant activities of both the total polysaccharides and four polysaccharide fractions were evaluated in vitro by radical hemolysis induced by H2O2 assays. The four fractions exhibited different antioxidant activities, and among them fraction S1 with mg/ml and 1.81 mg/ml, respectively and the highest reducing power and anti-lipid peroxidation induced by Fe2+-VC which reached 0.312 at 1 mg/ml and 45.67% at 0.5 mg/ml, respectively. The results showed that the antioxidant potential of polysaccharides from Dendrobium huoshanense is presumably related to their relative molecular mass.