湖南农业大学学报（自然科学版）
湖南農業大學學報（自然科學版）
호남농업대학학보（자연과학판）
JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCES)
2010年
1期
61-64
,共4页
背角无齿蚌%血细胞%形态特征%分类
揹角無齒蚌%血細胞%形態特徵%分類
배각무치방%혈세포%형태특정%분류
Anodonta woodiana%hemocytes%morphological features%classification
用光学显微镜和透射电子显微镜对背角无齿蚌血细胞进行观察.结果发现,体长6~12 cm的背角无齿蚌血细胞浓度为(2.42±0.38)×106/mL.根据细胞质内颗粒出现情况,将该蚌血细胞分为两大类,即颗粒细胞和无颗粒细胞.颗粒细胞明显的特征是细胞质中存在许多颗粒,无颗粒细胞的特征是细胞质中不含有或含有少量颗粒.根据颗粒细胞的体积和胞质内颗粒的大小还可以将其分为大颗粒细胞和小颗粒细胞(分别占4.28%和59.28%).根据无颗粒细胞的大小和核质比的不同将其分为透明细胞和淋巴样细胞(分别占27.74%与8.70%).4种类型的血细胞对Wrights染料有不同的亲和性,大颗粒细胞表现为嗜酸性,小颗粒细胞与透明细胞均呈现嗜酸性和嗜碱性两种,淋巴样细胞表现为嗜碱性.
用光學顯微鏡和透射電子顯微鏡對揹角無齒蚌血細胞進行觀察.結果髮現,體長6~12 cm的揹角無齒蚌血細胞濃度為(2.42±0.38)×106/mL.根據細胞質內顆粒齣現情況,將該蚌血細胞分為兩大類,即顆粒細胞和無顆粒細胞.顆粒細胞明顯的特徵是細胞質中存在許多顆粒,無顆粒細胞的特徵是細胞質中不含有或含有少量顆粒.根據顆粒細胞的體積和胞質內顆粒的大小還可以將其分為大顆粒細胞和小顆粒細胞(分彆佔4.28%和59.28%).根據無顆粒細胞的大小和覈質比的不同將其分為透明細胞和淋巴樣細胞(分彆佔27.74%與8.70%).4種類型的血細胞對Wrights染料有不同的親和性,大顆粒細胞錶現為嗜痠性,小顆粒細胞與透明細胞均呈現嗜痠性和嗜堿性兩種,淋巴樣細胞錶現為嗜堿性.
용광학현미경화투사전자현미경대배각무치방혈세포진행관찰.결과발현,체장6~12 cm적배각무치방혈세포농도위(2.42±0.38)×106/mL.근거세포질내과립출현정황,장해방혈세포분위량대류,즉과립세포화무과립세포.과립세포명현적특정시세포질중존재허다과립,무과립세포적특정시세포질중불함유혹함유소량과립.근거과립세포적체적화포질내과립적대소환가이장기분위대과립세포화소과립세포(분별점4.28%화59.28%).근거무과립세포적대소화핵질비적불동장기분위투명세포화림파양세포(분별점27.74%여8.70%).4충류형적혈세포대Wrights염료유불동적친화성,대과립세포표현위기산성,소과립세포여투명세포균정현기산성화기감성량충,림파양세포표현위기감성.
The light microscope and transmission electron microscope were used to obseve hemocytes of A.woodiana.The results shows that the total hemocyte content of A.woodiana was about (2.42±0.38)×106/mL from 6 to 12 centimeters in shell length.Two types of hemocytes were recognized: granular hemocytes and agranular hemocytes,based on either presence or absence of granules in the cytoplasm.Granular hemocytes are distinguished from other hemocytes by a positive feature,the possession of numerous cytoplasmic granules,while agranular hemocytes have few or a few electron-dense cytoplasm granules.In addition,acCoγding to the diameter of granular hemocytes and granular size,granular hemocytes were further divided into two categories: large granular hemocytes and small granular hemocytes,about 4.28% and 59.28% in the hemocyte population,respectively.In acCoγdance with the diameter of agranular hemocytes or N/C ratios,agranular hemocytes were further classified into two categories: hyalinocytes and lymphoid hemocytes,about 27.74% and 8.70% in the hemocyte population,respectively.Four types of hemocytes had different tinctorial properties for Wright's staining in smear.Large granular hemocytes were acidophils.Both small granular hemocytes and hyalinocytes had two tinctorial properties,acidophils and basophils.Lymphoid hemocytes were only basophils.
