CAJ | 학술논문

目的对不同品系大鼠肝卵圆细胞增殖模型进行比较,筛选建模效率较高的大鼠品系.方法选择F344、Wistar和SD大鼠各10只,经2-乙酰氨基芴灌胃和四氯化碳腹腔注射建立肝脏卵圆细胞增殖模型,组织学和免疫组织化学检测鉴定.荧光激活细胞筛选法分选并纯化各品系大鼠肝脏卵圆细胞,分析Thy-1.1~+细胞百分率,观察Thy-1.1~+细胞培养结果.结果成功建立三种品系大鼠肝脏卵圆细胞增殖模型.流式细胞仪分选结果显示,F344、wistar和SD大鼠Thy-1.1~+细胞百分率分别为(9.46±0.99)%、(2.46±0.37)%和(1.46±0.12)%;各品系大鼠间Thy-1.1~+细胞百分率比较,差异有统计学意义(P<0.05).细胞培养观察发现,与Wistar和SD大鼠比较,F344大鼠分选得到的肝脏卵圆细胞生长状态好且纯度较高.结论在用于建立肝脏卵圆细胞增殖模型最为常见的三种品系大鼠中,F344大鼠所建模型的肝脏卵圆细胞活化效率最高.
목적 대불동품계대서간란원세포증식모형진행비교,사선건모효솔교고적대서품계.방법 선택F344、Wistar화SD대서각10지,경2-을선안기물관위화사록화탄복강주사건립간장란원세포증식모형,조직학화면역조직화학검측감정.형광격활세포사선법분선병순화각품계대서간장란원세포,분석Thy-1.1~+세포백분솔,관찰Thy-1.1~+세포배양결과.결과 성공건립삼충품계대서간장란원세포증식모형.류식세포의분선결과현시,F344、wistar화SD대서Thy-1.1~+세포백분솔분별위(9.46±0.99)%、(2.46±0.37)%화(1.46±0.12)%;각품계대서간Thy-1.1~+세포백분솔비교,차이유통계학의의(P<0.05).세포배양관찰발현,여Wistar화SD대서비교,F344대서분선득도적간장란원세포생장상태호차순도교고.결론 재용우건립간장란원세포증식모형최위상견적삼충품계대서중,F344대서소건모형적간장란원세포활화효솔최고.
Objective To select the rat strain relatively more suitable for model establishment by comparison of hepatic oval cell proliferation models from different rat strains. Methods Ten F344 rats, 10 Wistar rats and 10 SD rats were selected, hepatic oval cell proliferation models were established respectively by intragastric administration of 2-acetylaminofluorene and intraperitoneally injection of carbon tetrachloride, and were identified by histological examination and immunohistochemical examination. Fluorescence-activated cell sorting was employed to separate and purify the hepatic oval cells from different rat strains, percentages of Thy-1. 1~+ cells were analysed, and culture results of Thy-1. 1~+ cells were observed. Results Hepatic oval cell proliferation models from three rat strains were successfully established. Fluorescence-activated cell sorting revealed that the percentages of Thy-1. 1~+ cells in F344 rats, Wistar Rats and SD rats were (9. 46 ± 0. 99) % , (2. 46 ± 0. 37) % and (1.46 ±0. 12) % , respectively, and there were significant differences in percentages of Thy-1. 1~+ cells among different rat strains (P <0.05). Observation of cell culture indicated that compared with Wistar rats and SD rats, hepatic oval cells separated from F344 rats were in better growth state and higher purifity. Conclusion Among the three rat strains most commonly used for hepatic oval cell proliferation models, hepatic oval cells from F344 rats have the highest activation efficiency.