中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
2期
162-167
,共6页
吴玉婷%陈佳%刘晓丽%徐利芬%黄崧凇%孟青
吳玉婷%陳佳%劉曉麗%徐利芬%黃崧凇%孟青
오옥정%진가%류효려%서리분%황숭송%맹청
糖尿病,实验性%心肌疾病%半酰氨酸天冬氨酸蛋白酶8%蛋白激酶C%细胞凋亡
糖尿病,實驗性%心肌疾病%半酰氨痠天鼕氨痠蛋白酶8%蛋白激酶C%細胞凋亡
당뇨병,실험성%심기질병%반선안산천동안산단백매8%단백격매C%세포조망
Diabetes mellitus,experimental%Cardiomyopathes%Caspase-8%Protein kinase C%Apoptosis
目的 通过研究糖尿病大鼠心脏中的细胞凋亡,Caspase-8及蛋白激酶C(PKC)-β的表达来探讨糖尿病心肌病(DCM)的组织学改变与细胞分子水平改变的相互关系.方法 实验大鼠分为4组:(1)糖尿病组,64只,用高糖高脂饲料喂养,予链脲佐菌素(STZ)30 mg/L一次性腹腔注射复制糖尿病模型.(2)正常对照组,37只,饲以普通饲料,腹腔注射柠檬酸盐缓冲液代替STZ.(3)STZ组,42只,饲以普通饲料,腹腔注射STZ.(4)高糖高脂组,37只,饲以高糖高脂饲料,腹腔注射柠檬酸盐缓冲液.在不同时段,抽样检查动物:观察心脏病理组织学变化;用实时荧光定量(PCR)等技术检测心肌Caspase-8及PKC-β的表达;用流式细胞术检测心肌的凋亡.比较不同组间的差异.结果 (1)造模型成功后,糖尿病组大鼠体质量较对照组明显下降(P＜0.05),且随着病程的进展呈下降趋势.高糖高脂组体质量一直较高,各时间段无明显改变.糖尿病组的血糖一直都高于对照组、STZ组及高糖高脂组(P＜0.05);高糖高脂组和对照组血糖差异无统计学意义.(2)糖尿病组细胞凋亡有随着病程延长而增高的趋势.(3)造模型成功后,Caspase-8、PKC-β的表达,糖尿病组高于对照组,而且有随着病程的延长增加的趋势.(4)糖尿病组发生心肌肥大、纤维化,随着病程的延长,病变有逐渐加重的病理特点.(5)相关分析提示,糖尿病组16周时,血糖和心肌细胞凋亡与Caspase-8、PKC-β的表达呈正相关.结论 Caspase-8表达失衡与DCM的发病有关.PKC-β在DCM中起到了一定的作用.高血糖在DCM的发病中起重要的作用.
目的 通過研究糖尿病大鼠心髒中的細胞凋亡,Caspase-8及蛋白激酶C(PKC)-β的錶達來探討糖尿病心肌病(DCM)的組織學改變與細胞分子水平改變的相互關繫.方法 實驗大鼠分為4組:(1)糖尿病組,64隻,用高糖高脂飼料餵養,予鏈脲佐菌素(STZ)30 mg/L一次性腹腔註射複製糖尿病模型.(2)正常對照組,37隻,飼以普通飼料,腹腔註射檸檬痠鹽緩遲液代替STZ.(3)STZ組,42隻,飼以普通飼料,腹腔註射STZ.(4)高糖高脂組,37隻,飼以高糖高脂飼料,腹腔註射檸檬痠鹽緩遲液.在不同時段,抽樣檢查動物:觀察心髒病理組織學變化;用實時熒光定量(PCR)等技術檢測心肌Caspase-8及PKC-β的錶達;用流式細胞術檢測心肌的凋亡.比較不同組間的差異.結果 (1)造模型成功後,糖尿病組大鼠體質量較對照組明顯下降(P＜0.05),且隨著病程的進展呈下降趨勢.高糖高脂組體質量一直較高,各時間段無明顯改變.糖尿病組的血糖一直都高于對照組、STZ組及高糖高脂組(P＜0.05);高糖高脂組和對照組血糖差異無統計學意義.(2)糖尿病組細胞凋亡有隨著病程延長而增高的趨勢.(3)造模型成功後,Caspase-8、PKC-β的錶達,糖尿病組高于對照組,而且有隨著病程的延長增加的趨勢.(4)糖尿病組髮生心肌肥大、纖維化,隨著病程的延長,病變有逐漸加重的病理特點.(5)相關分析提示,糖尿病組16週時,血糖和心肌細胞凋亡與Caspase-8、PKC-β的錶達呈正相關.結論 Caspase-8錶達失衡與DCM的髮病有關.PKC-β在DCM中起到瞭一定的作用.高血糖在DCM的髮病中起重要的作用.
목적 통과연구당뇨병대서심장중적세포조망,Caspase-8급단백격매C(PKC)-β적표체래탐토당뇨병심기병(DCM)적조직학개변여세포분자수평개변적상호관계.방법 실험대서분위4조:(1)당뇨병조,64지,용고당고지사료위양,여련뇨좌균소(STZ)30 mg/L일차성복강주사복제당뇨병모형.(2)정상대조조,37지,사이보통사료,복강주사저몽산염완충액대체STZ.(3)STZ조,42지,사이보통사료,복강주사STZ.(4)고당고지조,37지,사이고당고지사료,복강주사저몽산염완충액.재불동시단,추양검사동물:관찰심장병리조직학변화;용실시형광정량(PCR)등기술검측심기Caspase-8급PKC-β적표체;용류식세포술검측심기적조망.비교불동조간적차이.결과 (1)조모형성공후,당뇨병조대서체질량교대조조명현하강(P＜0.05),차수착병정적진전정하강추세.고당고지조체질량일직교고,각시간단무명현개변.당뇨병조적혈당일직도고우대조조、STZ조급고당고지조(P＜0.05);고당고지조화대조조혈당차이무통계학의의.(2)당뇨병조세포조망유수착병정연장이증고적추세.(3)조모형성공후,Caspase-8、PKC-β적표체,당뇨병조고우대조조,이차유수착병정적연장증가적추세.(4)당뇨병조발생심기비대、섬유화,수착병정적연장,병변유축점가중적병리특점.(5)상관분석제시,당뇨병조16주시,혈당화심기세포조망여Caspase-8、PKC-β적표체정정상관.결론 Caspase-8표체실형여DCM적발병유관.PKC-β재DCM중기도료일정적작용.고혈당재DCM적발병중기중요적작용.
Objective To study the roles of abnormal expressions of Caspase-8 and protein kinase C(PKC)-β of cardiomyocytes in the development of the apoptosis of cardiomyocyte in diabetic rat. Methods Rats were divided into 4 groups:(1)normal control (NC, n=37),(2)rats given STZ injection and normal diet(STZ,n= 42), (3) rats fed with high fat and high sngar ( HFS, n= 37), (4)rats given STZ injection and high fat and high sugar diet (type 2 DM, n=64). Plasma glucose, insulin and lipids were detected. At the end of experiment, the animals were sacrificed, and their hearts were examined. Pathological changes were observed and the expressions of Caspase-8, PKC-β mRNA were determined by real time-PCR method; apoptosis was analyzed by flow cytometry. Results (1)The body weight was higher in HFS group than in other three groups, and progressively decreased in type 2 diabetes group. The glucose level was highest in diabetic group, and was similar between groups of HFS and NC. (2)The apoptosis showed tendency to ascend during course of disease in diabetes model group. (3)The expressions of Caspase-8 and PKC-β mRNA were significantly enhanced in diabetes model group than in normal control group, and had a tendency to ascend during the course of disease.(4)The myocardial cells of the diabetic rats were rarified and swelling, fibrosis was observed. (5)At the 16th week, the level of plasma glucose was correlated positively with the expressions of Caspase-8 and PKC-β mRNA. Conclusions The enhancement of expressions of Caspase-8 amd PKC-β may play iportat rols in the pathogenesis of diabetic cardiomyopathy,in which apoptosis of the cardiomyocytes increased.
