CAJ | 학술논문

目的探讨人脑胶质瘤组织中白介素-17(IL-17)与转化生长因子β1(TGF-β1)表达的相关性及临床意义.方法应用免疫组化二步法检测135例脑胶质瘤、15例正常脑组织中IL-17与TGF-β1的表达.结果 IL-17在正常脑组织中未见表达,在高、低级别胶质瘤中表达差异有统计学意义(P<0.01),其在高级别胶质瘤中的表达明显高于低级别胶质瘤.胶质瘤组织中TGF-131高表达77例,低表达58例,高表达率57.03%.高级别胶质瘤组织中TGF-β1的表达明显高于低级别(P＜0.05),正常脑组织中未见表达.胶质瘤组织中IL-17与TGF-β1表达呈正相关(r=0.285,P＜0.01).Kaplan-Meier生存分析提示IL-17、TGF-β1高表达组生存时间与低表达组生存时间差异有统计学意义(P＜0.01或P＜0.05).Cox模型多因素分析显示年龄、KPS评分、IL-17是影响胶质瘤患者术后生存时间的独立预后因素(P＜0.01).结论人脑胶质瘤组织中IL-17的表达可能与TGF-β1有关.IL-17、TGF-β1的表达与人脑胶质瘤的恶性程度及预后相关.
목적 탐토인뇌효질류조직중백개소-17(IL-17)여전화생장인자β1(TGF-β1)표체적상관성급림상의의.방법 응용면역조화이보법검측135례뇌효질류、15례정상뇌조직중IL-17여TGF-β1적표체.결과 IL-17재정상뇌조직중미견표체,재고、저급별효질류중표체차이유통계학의의(P<0.01),기재고급별효질류중적표체명현고우저급별효질류.효질류조직중TGF-131고표체77례,저표체58례,고표체솔57.03%.고급별효질류조직중TGF-β1적표체명현고우저급별(P＜0.05),정상뇌조직중미견표체.효질류조직중IL-17여TGF-β1표체정정상관(r=0.285,P＜0.01).Kaplan-Meier생존분석제시IL-17、TGF-β1고표체조생존시간여저표체조생존시간차이유통계학의의(P＜0.01혹P＜0.05).Cox모형다인소분석현시년령、KPS평분、IL-17시영향효질류환자술후생존시간적독립예후인소(P＜0.01).결론 인뇌효질류조직중IL-17적표체가능여TGF-β1유관.IL-17、TGF-β1적표체여인뇌효질류적악성정도급예후상관.
Objective To investigate correlation between the amount of interleukin-17 (IL-17) producing cells and the expression of transforming growth factor (31 (TGF-β1) in glioma,and evaluate the clinical values of IL-17 and TGF-pl in predicting the prognosis of glioma. Methods The presence of IL-17 and TGF-pl was measured by immunohistochemistry in 135 human glioma (WHO Ⅰ 18,WHO Ⅱ 45,WHO Ⅲ 53,WHO Ⅳ 19) tissues and 15 normal brain tissues. Results There was no IL-17 positive staining in normal brain tissues. Of 135 glioma specimens showed low TGF-pl expression and 77 (57. 03% ) showed high TGF-pl expression. No TGF-β1 expression was detected in normal brain tissue. Furthermore,TGF-β1 expression was positively correlated with the amount of IL-17 producing cells in glioma tissues ( r=0.285, P<0.01). Compared with the low grade,the levels of IL-17 and TGF-pl positive cells were obviously increased in high grade. The Kaplan-Meier analysis showed that there were significant differences in overall survival (OS) between the IL-17 and TGF-pl high-expression and lowexpression group (P＜0.01, P＜0.05). The 3-year OS rates of IL-17 of high expression and low expression were 33.75% and 76. 36%. Multivariate Cox proportional hazards regression analysis indicated that age,KPS score, IL-17 were independent prognostic factor for OS (P＜0.01). Conclusion Intratumoral IL-17-producing cell density and the expression of TGF-β1 was associated with the malignancy of human glioma.