中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
z1期
45-49
,共5页
天疱疮%桥粒芯糖蛋白质类%酶联免疫吸附测定%自身抗体
天皰瘡%橋粒芯糖蛋白質類%酶聯免疫吸附測定%自身抗體
천포창%교립심당단백질류%매련면역흡부측정%자신항체
Pemphigus%Desmogleins%Enzyme-linked immunosorbent assay%Autoantibodies
目的 研究桥粒芯糖蛋白-酶联免疫吸附测定(desmoglein enzyme-linked immunosorbent assay,Dsg-ELISA)法检测寻常型天疱疮(pemphigus vulgaris,PV)抗体在PV诊断中的作用,以期为临床提供参考.方法 用Dsg-ELISA、猴食道为底物的间接免疫荧光法(monkey oesphagus-indirectimmunofluoreseent assay,MO-IIF)检测44例PV患者和29例非天疱疮口腔黏膜病患者血清抗体,比较两种方法的灵敏度和特异度.通过Dsg-ELISA法检测Dsg-1,3抗体水平,研究抗体水平与皮肤口腔黏膜病损严重程度的关系.结果 Dsg-3-ELISA灵敏度89％( 39/44),特异度93％(27/29)；MO-IIF灵敏度93％ (41/44),特异度83％ (24/29),两种方法的灵敏度和特异度相比差异均无统计学意义(P＞0.05)；Dsg-3抗体水平与口腔黏膜病损严重程度呈正相关(P＜0.01),与皮肤病损严重程度无相关性(P＞0.05)；Dsg-1抗体水平与皮肤病损严重程度呈正相关(P＜0.01),与口腔黏膜病损严重程度无相关性(P＞0.05).结论 MO-IIF、Dsg-3-ELISA是PV敏感性和特异性高的诊断方法；Dsg-ELISA是PV血清学诊断的有效工具,也可以作为PV病情判断的工具.
目的 研究橋粒芯糖蛋白-酶聯免疫吸附測定(desmoglein enzyme-linked immunosorbent assay,Dsg-ELISA)法檢測尋常型天皰瘡(pemphigus vulgaris,PV)抗體在PV診斷中的作用,以期為臨床提供參攷.方法 用Dsg-ELISA、猴食道為底物的間接免疫熒光法(monkey oesphagus-indirectimmunofluoreseent assay,MO-IIF)檢測44例PV患者和29例非天皰瘡口腔黏膜病患者血清抗體,比較兩種方法的靈敏度和特異度.通過Dsg-ELISA法檢測Dsg-1,3抗體水平,研究抗體水平與皮膚口腔黏膜病損嚴重程度的關繫.結果 Dsg-3-ELISA靈敏度89％( 39/44),特異度93％(27/29)；MO-IIF靈敏度93％ (41/44),特異度83％ (24/29),兩種方法的靈敏度和特異度相比差異均無統計學意義(P＞0.05)；Dsg-3抗體水平與口腔黏膜病損嚴重程度呈正相關(P＜0.01),與皮膚病損嚴重程度無相關性(P＞0.05)；Dsg-1抗體水平與皮膚病損嚴重程度呈正相關(P＜0.01),與口腔黏膜病損嚴重程度無相關性(P＞0.05).結論 MO-IIF、Dsg-3-ELISA是PV敏感性和特異性高的診斷方法；Dsg-ELISA是PV血清學診斷的有效工具,也可以作為PV病情判斷的工具.
목적 연구교립심당단백-매련면역흡부측정(desmoglein enzyme-linked immunosorbent assay,Dsg-ELISA)법검측심상형천포창(pemphigus vulgaris,PV)항체재PV진단중적작용,이기위림상제공삼고.방법 용Dsg-ELISA、후식도위저물적간접면역형광법(monkey oesphagus-indirectimmunofluoreseent assay,MO-IIF)검측44례PV환자화29례비천포창구강점막병환자혈청항체,비교량충방법적령민도화특이도.통과Dsg-ELISA법검측Dsg-1,3항체수평,연구항체수평여피부구강점막병손엄중정도적관계.결과 Dsg-3-ELISA령민도89％( 39/44),특이도93％(27/29)；MO-IIF령민도93％ (41/44),특이도83％ (24/29),량충방법적령민도화특이도상비차이균무통계학의의(P＞0.05)；Dsg-3항체수평여구강점막병손엄중정도정정상관(P＜0.01),여피부병손엄중정도무상관성(P＞0.05)；Dsg-1항체수평여피부병손엄중정도정정상관(P＜0.01),여구강점막병손엄중정도무상관성(P＞0.05).결론 MO-IIF、Dsg-3-ELISA시PV민감성화특이성고적진단방법；Dsg-ELISA시PV혈청학진단적유효공구,야가이작위PV병정판단적공구.
Objective To investigate the effect of the enzyme-linked immunosorbent assay(ELISA) and indirect immunofluorescent(IIF) on the diagnosis and monitoring of the activity of pemphigus vulgaris (PV).Methods Serum antibody titers of 44 PV patients and 29 none-PV patients were detected by desmoglein(Dsg)-1,3-ELISA and monkey oesphagus-IIF (MO-IIF).The sensitivity and specificity of the ELISA and MO-IIF were compared in the diagnosis of pemphigus vulgaris.The correlation between serum level of anti-desmoglein antibodies and the disease severity was analyzed.Results The sensitivity of MO-IIF was 93％ (41/44) and the specificity was 83 ％ (24/29).The sensitivity of Dsg-3-ELISA was 89％ (39/44) and the specificity was 93％ (27/29).There was no significant difference between MO-IIF and Dsg-3-ELISA (P ＞ 0.05 ).Dsg-3 antibody levels showed positive correlation with oral disease severity (P ＜ 0.01 )and no correlation with skin disease severity in pemphigus vulgaris( P ＜0.05 ).Dsg-1 antibody levels showed positive correlation with skin disease severity ( P ＜ 0.01 ) and no correlation with oral disease severity in pemphigus vulgaris(P ＜ 0.05 ).Conclusions Dsg-3-ELISA and MO- Ⅱ F methods were highly sensitive and specific in the diagnosis of PV.Dsg-1,3-ELISA is the useful tool for the sero-diagnosis and monitoring of the activity of PV.
