CAJ | 학술논문

目的探讨延髓内脏带(MVZ)与下丘脑室旁核(PVN)和视上核(SON)之间是否存在往返渗透压投射通路.方法通过给予大鼠饮用3%氯化钠的方法制作高渗刺激模型,并用WGA-HRP逆行追踪、抗Fos、抗酪氨酸羟化酶(TH)或加压素(VP)及胶质纤维酸性蛋白(GFAP)免疫组织化学相结合的四重标记方法,观察MVZ、PVN和SON中WGA-HRP、Fos、TH、VP和GFAP阳性分布及表达状况.结果高渗刺激后MVZ、PVN和SON内Fos阳性细胞明显增多;GFAP阳性结构也明显增多,其分布与Fos阳性细胞分布基本一致,表现为胞体肥大、突起粗长.星形胶质细胞(AST)紧密包绕在神经元周围形成神经元-AST复合体(N-ASC).结论神经元和AST以N-ASC的形式共同参与渗透压调节反应,体内存在MVZ和SON或PVN之间往返的渗透压调节通路.
목적 탐토연수내장대(MVZ)여하구뇌실방핵(PVN)화시상핵(SON)지간시부존재왕반삼투압투사통로.방법 통과급여대서음용3%록화납적방법제작고삼자격모형,병용WGA-HRP역행추종、항Fos、항락안산간화매(TH)혹가압소(VP)급효질섬유산성단백(GFAP)면역조직화학상결합적사중표기방법,관찰MVZ、PVN화SON중WGA-HRP、Fos、TH、VP화GFAP양성분포급표체상황.결과 고삼자격후MVZ、PVN화SON내Fos양성세포명현증다;GFAP양성결구야명현증다,기분포여Fos양성세포분포기본일치,표현위포체비대、돌기조장.성형효질세포(AST)긴밀포요재신경원주위형성신경원-AST복합체(N-ASC).결론 신경원화AST이N-ASC적형식공동삼여삼투압조절반응,체내존재MVZ화SON혹PVN지간왕반적삼투압조절통로.
Objective To test the possibility of reciprocal pathways between medullary visceral zone (MVZ) and hypothalamic paraventricular nucleus (PVN) or supraoptic nucleus (SON) following hyperosmotic stimulation. Methods Hyperosmotic pressure animal model was established by administering3% sodium chloride as drinking water to rats. The distributions and expressions ofHRP retrogradely labeled neurons, Fos, tyrosine hydroxylase (TH) or vasopressin (VP) positive neuron and lial fibrillary acidic protein (GFAP) positive astrocytes (AST) in MVZ, SON and PVN were observed by quadruple labeling methods of WGA-HRP retrograde tracing combined with anti-Fos, TH (or VP) and GFAP immunohistochemical technique. Results Fos positive neurons within the MVZ, PVN and SON increased markedly. There were also a large number of GFAP positive structures in the brain and their distribution pattern was fundamentally similar or analogous to Fos positive neurons in the above-mentioned areas. The augmented GFAP reactivities took on hypertrophic cell bodies, thicker and longer processes.Quadruplicate immunohistochemical staining showed that a neuron could be closely surrounded by many AST and they formed neuron-astrocytic complex (N-ASC). Conclusion The neurons and AST might be very active following hyperosmotic pressure and N-ASC as a functional unit might serve to modulate the osmotic pressure. There was reciprocal osmoregulation pathways between the MVZ and SON or PVN in the brain.