中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2011年
8期
544-549
,共6页
卒中%抑郁%多态现象,遗传%受体,血清素,5-HT1A%异源三聚体GTP结合蛋白质类
卒中%抑鬱%多態現象,遺傳%受體,血清素,5-HT1A%異源三聚體GTP結閤蛋白質類
졸중%억욱%다태현상,유전%수체,혈청소,5-HT1A%이원삼취체GTP결합단백질류
Stroke%Depression%Polymorphisms,genetic%Receptor,serotonin,5-HT1 A%Heterotrimeric GTP-binding proteins
目的 观察5-羟色胺1A受体(5-HTR1A)C(-1019)G基因与G蛋白β3亚基(GNβ3)基因C825T多态性在中国广州地区卒中后抑郁患者的分布情况及特点,探讨卒中后抑郁的遗传机制.方法 选取159例首发脑卒中患者并根据汉密尔顿抑郁量表(HAMD)评分分为卒中后抑郁组(53例)和卒中对照组(106例),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析2组患者的5-HTR1A C(-1019)G和GNβ3 C825T基因多态性.结果 5-HTR1A C(-1019)G和GNβ3 C825T基因多态性在2组人群中的分布差异均有统计学意义,卒中后抑郁组5-HTR1A(-1019)GG基因型(8/53,15.1%)及G等位基因频率(44/106,41.5%)和GNβ3 825T等位基因频率(68/106,64.2%)均高于对照组(5/106,4.7%;35/212,16.5%;113/212,53.3%;×2=23.204、23.655、3.392,均P＜0.05).同时携带5-HTR1A(-1019)G和GNβ3 825T等位基因者罹患卒中后抑郁的相对危险度(OR=4.980,95%CI 2.429～10.210,P=0.000)比单独具有5-HTR1A(-1019)G等位基因者(OR=3.589,95%CI2.113～6.096,P=0.000)或GNβ3 825T等位基因者高(OR=0.638,95%CI 0.395～1.031,P=0.042).结论 5-HTR1A C(-1019)G和GNβ3 C825T基因均可能是卒中后抑郁的易患基因,而且两者在卒中后抑郁的发病中存在微效协同作用.
目的 觀察5-羥色胺1A受體(5-HTR1A)C(-1019)G基因與G蛋白β3亞基(GNβ3)基因C825T多態性在中國廣州地區卒中後抑鬱患者的分佈情況及特點,探討卒中後抑鬱的遺傳機製.方法 選取159例首髮腦卒中患者併根據漢密爾頓抑鬱量錶(HAMD)評分分為卒中後抑鬱組(53例)和卒中對照組(106例),採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)技術分析2組患者的5-HTR1A C(-1019)G和GNβ3 C825T基因多態性.結果 5-HTR1A C(-1019)G和GNβ3 C825T基因多態性在2組人群中的分佈差異均有統計學意義,卒中後抑鬱組5-HTR1A(-1019)GG基因型(8/53,15.1%)及G等位基因頻率(44/106,41.5%)和GNβ3 825T等位基因頻率(68/106,64.2%)均高于對照組(5/106,4.7%;35/212,16.5%;113/212,53.3%;×2=23.204、23.655、3.392,均P＜0.05).同時攜帶5-HTR1A(-1019)G和GNβ3 825T等位基因者罹患卒中後抑鬱的相對危險度(OR=4.980,95%CI 2.429～10.210,P=0.000)比單獨具有5-HTR1A(-1019)G等位基因者(OR=3.589,95%CI2.113～6.096,P=0.000)或GNβ3 825T等位基因者高(OR=0.638,95%CI 0.395～1.031,P=0.042).結論 5-HTR1A C(-1019)G和GNβ3 C825T基因均可能是卒中後抑鬱的易患基因,而且兩者在卒中後抑鬱的髮病中存在微效協同作用.
목적 관찰5-간색알1A수체(5-HTR1A)C(-1019)G기인여G단백β3아기(GNβ3)기인C825T다태성재중국엄주지구졸중후억욱환자적분포정황급특점,탐토졸중후억욱적유전궤제.방법 선취159례수발뇌졸중환자병근거한밀이돈억욱량표(HAMD)평분분위졸중후억욱조(53례)화졸중대조조(106례),채용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술분석2조환자적5-HTR1A C(-1019)G화GNβ3 C825T기인다태성.결과 5-HTR1A C(-1019)G화GNβ3 C825T기인다태성재2조인군중적분포차이균유통계학의의,졸중후억욱조5-HTR1A(-1019)GG기인형(8/53,15.1%)급G등위기인빈솔(44/106,41.5%)화GNβ3 825T등위기인빈솔(68/106,64.2%)균고우대조조(5/106,4.7%;35/212,16.5%;113/212,53.3%;×2=23.204、23.655、3.392,균P＜0.05).동시휴대5-HTR1A(-1019)G화GNβ3 825T등위기인자리환졸중후억욱적상대위험도(OR=4.980,95%CI 2.429～10.210,P=0.000)비단독구유5-HTR1A(-1019)G등위기인자(OR=3.589,95%CI2.113～6.096,P=0.000)혹GNβ3 825T등위기인자고(OR=0.638,95%CI 0.395～1.031,P=0.042).결론 5-HTR1A C(-1019)G화GNβ3 C825T기인균가능시졸중후억욱적역환기인,이차량자재졸중후억욱적발병중존재미효협동작용.
Objective To assess whether 5-HTR1A C( - 1019) G and GNβ3 C825T gene polymorphisms are associated with post-stroke depression (PSD) and explore the genetic mechanism of the pathogenesis of post-stroke depression. Methods All 159 patients with first stroke were divided into the PSD group and the control group according to HAMD scores. Their genotypes were determined with polymerase chain reaction and allele-specific restriction enzyme analysis. Results The frequency of 5-HTR1A (-1019) GG genotype(8/53,15. 1% ), G allele (44/106,41.5%)and GNβ3 825T allele(68/106,64. 2% ) were significantly higher in the post-stroke depression group than in the controls (5/106,4.7% ;35/212, 16. 5%; 113/212, 53.3%; ×2 = 23.204, 23. 655, 3. 392, all P ＜ 0. 05 ). Combined genotype analysis showed that individuals with both 5-HTR1A ( - 1019) G and GNβ3 825T allele ( OR =4. 980,95% CI 2. 429-10. 210,P =0. 000) had a higher risk than those with 5-HTR1 A (-1019) G allele ( OR = 3. 589,95% CI 2. 113-6. 096, P = 0. 000) or GNβ3 825T allele ( OR = 0. 638,95% CI 0.395-1. 031 ,P =0. 042) only for post-stroke depression. Conclusion The 5-HTR1A C( - 1019)G and GNβ3 C825T polymorphisms are predisposing genes of post-stroke depression. Our data also suggest a significant interaction between the 5-HTR1A ( - 1019)G allele and GNβ3 825T allele in post-stroke depression.
