CAJ | 학술논문

目的研究选择性环氧合酶-2(COX-2)抑制剂尼美舒利(NIM)对人类舌鳞癌Tca-8113细胞血管生成素-1、2(Ang-1、Ang-2)蛋白表达的影响.方法体外培养人类舌鳞癌Tca-8113细胞,加入不同浓度的NIM(0、25、50、100、200 μmol/L)进行干预,分别作用24、48、72 h后,放射免疫法检测细胞培养上清液中PGE2分泌水平的改变,免疫组织化学(SP)方法检测人类舌鳞癌Tca-8113细胞内Ang-1和Ang-2蛋白表达的变化情况.结果 NIM呈浓度和时间依赖性地抑制PGE2分泌和Ang-2蛋白的表达,Ang-1蛋白表达受NIM影响不明显.结论 NIM可减少PGE2分泌和Ang-2蛋白的表达,此机制可能是通过COX-2依赖性肿瘤血管生成抑制途径起作用.
목적 연구선택성배양합매-2(COX-2)억제제니미서리(NIM)대인류설린암Tca-8113세포혈관생성소-1、2(Ang-1、Ang-2)단백표체적영향.방법 체외배양인류설린암Tca-8113세포,가입불동농도적NIM(0、25、50、100、200 μmol/L)진행간예,분별작용24、48、72 h후,방사면역법검측세포배양상청액중PGE2분비수평적개변,면역조직화학(SP)방법검측인류설린암Tca-8113세포내Ang-1화Ang-2단백표체적변화정황.결과 NIM정농도화시간의뢰성지억제PGE2분비화Ang-2단백적표체,Ang-1단백표체수NIM영향불명현.결론 NIM가감소PGE2분비화Ang-2단백적표체,차궤제가능시통과COX-2의뢰성종류혈관생성억제도경기작용.
Objective To investigate the influence of Nimesulide(NIM),a selective Cyclooxygenase-2 (COX-2) inhibitor, on the expression of Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) protein in human tongue squamous cell carcinoma Tca-8113 cell lines. Methods Tca-8113 cell were cultured in vitro, and exposed to a range of concentrations of nimesulide (NIM) (0, 25, 50, 100, 200 μmol/L) for 24 h, 48 h, 72 h, and the PGE2 level in the cells cultured supernate was quantitated by the radioimmunoassay, and the expression of Ang-1 and Ang-2 protein in human tongue squamous cell line Tca-8113 were measured by the SABC immunocytochemistry staining simultaneously. Results The PGE2 level in the cultured supematant and expression of Ang-2 in Tca-8113 cells decreased by NIM in a concentration and time dependent manner, but not Ang-1. Conclusion NIM can decrease PGE2 level and Ang-2 expression, which may be involved in the inhibition of cancer angiogenesis of COX-2 dependence mechanisms.