G-CSF或SCF体外刺激前后阵发性睡眠性血红蛋白尿症患者骨髓造血干细胞内磷酸化STAT5的表达
G-CSF혹SCF체외자격전후진발성수면성혈홍단백뇨증환자골수조혈간세포내린산화STAT5적표체
Expression of phosphorylated STAT5 in bone marrow hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria before and after in vitro G-CSF or SCF stimulation
  • 万方数据
    中华医学杂志 중화의학잡지
    National Medical Journal of China
    2012年 14期 956-959 ,共4页

    丁少雪%付蓉%王红蕾%张田%阮二宝%瞿文%梁勇%王国锦%王晓明%刘鸿%吴玉红%宋嘉%王化泉%邢莉民%关晶%王珺%李丽娟%邵宗鸿

    정소설%부용%왕홍뢰%장전%원이보%구문%량용%왕국금%왕효명%류홍%오옥홍%송가%왕화천%형리민%관정%왕군%리려연%소종홍

    血红蛋白尿,阵发性%造血干细胞%粒细胞集落刺激因子%干细胞因子%STAT5 血紅蛋白尿,陣髮性%造血榦細胞%粒細胞集落刺激因子%榦細胞因子%STAT5
    혈홍단백뇨,진발성%조혈간세포%립세포집락자격인자%간세포인자%STAT5
    Hemoglobinuria,paroxysmal%Hematopoietic stem cell%Granulocyte colony-stimulating factor%Stem cell factor%STAT5
    目的 观察阵发性睡眠性血红蛋白尿症(PNH)患者骨髓正常造血克隆(CD34+ CD59+)和异常造血克隆(CD34+ CD59-)细胞在粒细胞集落刺激因子(G-CSF)或干细胞因子(SCF)体外刺激后胞质内信号转导及转录激活子5(STAT5)磷酸化表达差异.方法 26例PNH患者及14名健康对照骨髓单个核细胞(BMMNC)经和不经100 ng/ml G-CSF、SCF刺激,应用蛋白磷酸化流式细胞术分别检测其中CD34+ CD59+和CD34+ CD59-细胞磷酸化STAT5(P-STAT5)的平均荧光强度(M FI).结果 (1)细胞因子刺激前,PNH患者CD34+ CD59-细胞内P-STAT5的MFI(单位:道数)为(24±18),明显低于CD34+ CD59+细胞(64±49)和健康对照CD34+ CD59+细胞(61±33)(均P＜0.01)；后两者间差异无统计学意义(P＞0.05).(2) G-CSF刺激后,PNH患者CD34+ CD59-细胞P-STAT5的MFI为(36±35),明显低于CD34+ CD59+细胞(124±84)和健康对照CD34+ CD59+细胞(116±59)(均P＜0.01)；后两者间差异无统计学意义(P＞0.05).(3) SCF刺激后,PNH患者CD34+ CD59-细胞P-STAT5的MFI为(34±27),明显低于CD34+ CD59+细胞(124±97)和健康对照CD34+ CD59+细胞(128±62)(均P＜0.01)；后两者间差异无统计学意义(P＞0.05).(4) G-CSF或SCF刺激后,PNH患者及健康对照CD34+ CD59+细胞P-STAT5的MFI较刺激前的增高程度,均明显超过PNH患者CD34+ CD59-细胞(均P＜0.01).结论 PNH克隆在体外应用G-CSF、SCF刺激后,胞内信号传导(STAT5)磷酸化明显低于正常造血克隆.
    목적 관찰진발성수면성혈홍단백뇨증(PNH)환자골수정상조혈극륭(CD34+ CD59+)화이상조혈극륭(CD34+ CD59-)세포재립세포집락자격인자(G-CSF)혹간세포인자(SCF)체외자격후포질내신호전도급전록격활자5(STAT5)린산화표체차이.방법 26례PNH환자급14명건강대조골수단개핵세포(BMMNC)경화불경100 ng/ml G-CSF、SCF자격,응용단백린산화류식세포술분별검측기중CD34+ CD59+화CD34+ CD59-세포린산화STAT5(P-STAT5)적평균형광강도(M FI).결과 (1)세포인자자격전,PNH환자CD34+ CD59-세포내P-STAT5적MFI(단위:도수)위(24±18),명현저우CD34+ CD59+세포(64±49)화건강대조CD34+ CD59+세포(61±33)(균P＜0.01)；후량자간차이무통계학의의(P＞0.05).(2) G-CSF자격후,PNH환자CD34+ CD59-세포P-STAT5적MFI위(36±35),명현저우CD34+ CD59+세포(124±84)화건강대조CD34+ CD59+세포(116±59)(균P＜0.01)；후량자간차이무통계학의의(P＞0.05).(3) SCF자격후,PNH환자CD34+ CD59-세포P-STAT5적MFI위(34±27),명현저우CD34+ CD59+세포(124±97)화건강대조CD34+ CD59+세포(128±62)(균P＜0.01)；후량자간차이무통계학의의(P＞0.05).(4) G-CSF혹SCF자격후,PNH환자급건강대조CD34+ CD59+세포P-STAT5적MFI교자격전적증고정도,균명현초과PNH환자CD34+ CD59-세포(균P＜0.01).결론 PNH극륭재체외응용G-CSF、SCF자격후,포내신호전도(STAT5)린산화명현저우정상조혈극륭.
    Objective To study the expressions of phosphorylated STAT5 (P-STAT5) in CD34+ CD59- and CD34+ CD59+ bone marrow cells of the patients with paroxysmal nocturnal hemoglobinuria (PNH) before and after in vitro G-CSF or SCF stimulation,then evaluate the functions of G-CSF and SCF receptors in PNII clone cells.Methods Bone marrow mononuclear cells (BMMNC) of 26 PNH patients and 14 normal controls were stimulated in vitro with G-CSF (100 ng/ml) or SCF (100 ng/ml) for 10 min.Before and after these stimulations,the mean fluorescence intensity (MFI) of P-STAT5 in CD34+ CD59+ BMMNC and CD34+ CD59- BMMNC were measured by flow cytometry.Results (1) The P-STAT5 MFI was (24±18) in unstimulated CD34+ CD59- cells.And it was significantly lower than that in unstimulated CD34+ CD59+ cells of PNH patients (64±49) and normal controls (61±33) (both P＜0.01).No statistic difference existed between the latter two.(2)The P-STAT5 MFI was (36±35) in G-CSF stimulated CD34+ CD59- cells of PNH patients.And it was significantly lower than that in G-CSF stimulated CD34+ CD59+ cells of PNH patients (124±84) and normal controls (116±59) (both P＜0.01).There was no statistic difference between the latter two.(3)The P-STAT5 MFI was (34±27) in SCF stimulated CD34+ CD59- cells of PNH patients.And it was significantly lower than that in SCF stimulated CD34+ CD59+ cells of PNH patients (124±97) and normal controls (128±62) (both P＜0.01).And no statistic difference existed between the latter two.(4)The incrcased P-STAT5 MFI in G-CSF stimulated CD34+ CD59- cells of PNH patients was significantly lower than that in G-CSF stimulated CD34+ CD59+ cells of PNH patients and normal controls (both P＜0.01).No statistic difference existcd between the latter two.The increase of P-STAT5 MFI in SCF stimulated CD34+ CD59- cells ofPNH patients was significantly lower than that in SCF stimulated CD34+ CD59+ cells of PNH patients and normal controls (both P＜0.01).No statistic difference existed between the latter two.Conclusion There is a lower expression of P-STAT5 expressed in G-CSF or SCF stimulated PNH clone cells compared to that in normal clone cells.
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