CAJ | 학술논문

实验表明：外施2 mg·L-1的激动素(Kinetin．KT)可以提高受冷害(4℃，36 h)稻苗体内SOD的活性，而不能提高未受冷害的正常稻苗及无细胞提取液的SOD活性。为了研究KT促进冷害稻苗SOD从头合成机理，本试验采用了转录抑制剂AMD(3mg·L-1)和翻译抑制剂CHI(0．5mg·L-1)处理受冷害稻苗。试验表明，激动素主要是在转录水平上促进SOD的从头合成。
실험표명：외시2 mg·L-1적격동소(Kinetin．KT)가이제고수냉해(4℃，36 h)도묘체내SOD적활성，이불능제고미수냉해적정상도묘급무세포제취액적SOD활성。위료연구KT촉진냉해도묘SOD종두합성궤리，본시험채용료전록억제제AMD(3mg·L-1)화번역억제제CHI(0．5mg·L-1)처리수냉해도묘。시험표명，격동소주요시재전록수평상촉진SOD적종두합성。
Experiments showed that exogenous KT(2 mg·L-1) can increase SOD activity of rice seedlings after chilling injury, but the SOD activity of normal rice seedlings and its cell free extract did not increased with KT treatment. For study on mechanism of de novo SOD biosynthesis of seedling after chilling injury with KT treatement, transcription inhibitor actinomycin D(AMD, 3 mg·L-1) was used. The experiments showed that KT promoted SOD de novo biosynthesis mainly on the transciriptional level.