CAJ | 학술논문

目的观察胰岛素对AD样模型大鼠认知、病理、生化指标的影响.方法采用立体定向双侧海马CA3区注射Aβ42建立AD大鼠模型,皮下注射胰岛素作为干预,观察大鼠Morris水迷宫逃避潜伏期、穿越平台次数,以及采用免疫组化和ELISA方法,比较脑内Aβ沉积的改变.结果 Aβ+胰岛素组与Aβ组相比,具有较多的海马Aβ沉积(增加35.7%),和更高的Aβ含量(增加23.6%),同时其逃避潜伏期明显延长,穿越平台次数显著减少.结论胰岛素加重AD样模型大鼠的Aβ沉积和认知损害.
목적관찰이도소대AD양모형대서인지、병리、생화지표적영향.방법채용입체정향쌍측해마CA3구주사Aβ42건립AD대서모형,피하주사이도소작위간예,관찰대서Morris수미궁도피잠복기、천월평태차수,이급채용면역조화화ELISA방법,비교뇌내Aβ침적적개변.결과 Aβ+이도소조여Aβ조상비,구유교다적해마Aβ침적(증가35.7%),화경고적Aβ함량(증가23.6%),동시기도피잠복기명현연장,천월평태차수현저감소.결론이도소가중AD양모형대서적Aβ침적화인지손해.
Objective to evaluate the impact of insulin on pathology and cognition in AD-like rat model. Methods 32 male Wistar rats were divided into 4 groups: Aβ, Aβ+ insulin, insulin and normal control group. Each group included 8 subjects. Aggregated Aβ solution was injected in CA3 area of bilateral hippocampi. Insulin was administered subcutaneously pre-surgery once a day for 7 consecutive days. Behavioral performance in Morris water maze was observed and level of Aβ deposition was assessed by immunohistochemistry staining and ELISA assay. Results Aβ+insulin group had a much higher Aβ level (increased by 23.6%) and more severe Aβ deposition (increased by 35.7%) than the other three groups. And poorer performance in Morris water maze was observed. Conclusion Insulin can aggravate Aβ deposition in the hippocampus of AD-like rat model and worsen its memory loss.