CAJ | 학술논문

目的:制备复方一枝蒿-壳聚糖纳米药物并研究其体外抗菌作用.方法: 通过乳化交联法合成复方一枝蒿-壳聚糖纳米药物.分别用激光粒度分析仪(Malvern Zetasizer 1000-HAS)和原子力显微镜(AFM)检测该种纳米药物的粒径分布以及表面电位.通过体外抗菌实验检测此种纳米药物的体外抗菌能力,并与传统复方一支蒿颗粒的抗菌能力作比较.结果: 该种纳米药物粒径分布均一,为(137.00±14.28) nm;表面携带正电荷,Zeta电位值为(16.90±1.32)mV.其对金黄色葡萄球菌、肺炎球菌、乙型溶血性链球菌和大肠杆菌的最小抑菌浓度分别为1:32,1:32,1:16 和 1:2,最小杀菌浓度分别为1:16,1:16,1:8和1:2.与传统复方一支蒿颗粒相比,该纳米药物对金黄色葡萄球菌、肺炎球菌及乙型溶血性链球菌的抗菌能力均有所改进(P<0.05).结论:复方一枝蒿-壳聚糖纳米药物对金黄色葡萄球菌、肺炎球菌、乙型溶血性链球菌均具有明显的体外抗菌作用,为我国传统中药新剂型的开发奠定了实验学基础.
목적:제비복방일지호-각취당납미약물병연구기체외항균작용.방법: 통과유화교련법합성복방일지호-각취당납미약물.분별용격광립도분석의(Malvern Zetasizer 1000-HAS)화원자력현미경(AFM)검측해충납미약물적립경분포이급표면전위.통과체외항균실험검측차충납미약물적체외항균능력,병여전통복방일지호과립적항균능력작비교.결과: 해충납미약물립경분포균일,위(137.00±14.28) nm;표면휴대정전하,Zeta전위치위(16.90±1.32)mV.기대금황색포도구균、폐염구균、을형용혈성련구균화대장간균적최소억균농도분별위1:32,1:32,1:16 화 1:2,최소살균농도분별위1:16,1:16,1:8화1:2.여전통복방일지호과립상비,해납미약물대금황색포도구균、폐염구균급을형용혈성련구균적항균능력균유소개진(P<0.05).결론:복방일지호-각취당납미약물대금황색포도구균、폐염구균、을형용혈성련구균균구유명현적체외항균작용,위아국전통중약신제형적개발전정료실험학기출.
Objective To prepare chitosan (CS)-compound Yizhihao-nanoparticles (NP) and to investigate its antibacterial activity. Methods CS NPs were formed by the incorporation of CS and Na3PO4. CS-compound Yizhihao NPs were prepared by ion-cross-linking. The particle sizes and surface charges of CS NPs were determined by Malvern Zetasizer 1000-HAS and atomic force microscope (AFM), respectively. The antibacterial acitivity of CS-compound Yizhihao-NPs was studied in vitro and compared with that of compound Yizhihao powder. Results Malvern Zetasizer 1000-HAS and AFM demonstrated that the diameter of CS-compound Yizhihao NPs was (137.00±14.28)nm and CS NPs had (16.90±1.32)mV positive surface charges. The minimal inhibitory concentrations (MIC) of CS-compound Yizhihao NPs on Staphylococcus aureus,Pneumococcus,β-hemolytic streptococcus, and Escherichia coli were 1:32,1:32,1:16,and 1:2,respectively. The minimal bactericidal concentrations (MBC) of CS-compound Yizhihao-NPs on Staphylococcus aureus,Pneumococcus,β-hemolytic streptococcus, and Escherichia coli were 1:16,1:16,1:8, and 1:2,respectively. The antibacterial efficacy of CS-compound Yizhihao-NPs to Staphylococcus aureus,Pneumococcus,and β-hemolytic streptococcus had been improved significantly (P<0.05). Conclusion CS-compound Yizhihao-nanoparticles have obvious antibacterial activity to the Staphylococcus aureus,Pneumococcus,and β-hemolytic streptococcus,which lays the experimental foundation for new preparation of traditional Chinese medicine in future research.